Cited by 579Neurogenesis and neuroplasticity mechanismsNeuroscience and Neuropharmacology ResearchMemory and Neural Mechanisms

Synapse formation on neurons born in the adult hippocampus

Toni, Nicolas, Teng, E Matthew, Bushong, Eric A, Aimone, James B, Zhao, Chunmei, Consiglio, Antonella, van Praag, Henriette, Martone, Maryann E +2 more·2007·Nature Neuroscience

Abstract

Although new and functional neurons are produced in the adult brain, little is known about how they integrate into mature networks. Here we explored the mechanisms of synaptogenesis on neurons born in the adult mouse hippocampus using confocal microscopy, electron microscopy and live imaging. We report that new neurons, similar to mature granule neurons, were contacted by axosomatic, axodendritic and axospinous synapses. Consistent with their putative role in synaptogenesis, dendritic filopodia were more abundant during the early stages of maturation and, when analyzed in three dimensions, the tips of all filopodia were found within 200 nm of preexisting boutons that already synapsed on other neurons. Furthermore, dendritic spines primarily synapsed on multiple-synapse boutons, suggesting that initial contacts were preferentially made with preexisting boutons already involved in a synapse. The connectivity of new neurons continued to change until at least 2 months, long after the formation of the first dendritic protrusions.

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Crossref · 10.1038/nn1908

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Authors
Toni, N., Teng, E. M., Bushong, E. A., Aimone, J. B., Zhao, C., Consiglio, A., van Praag, H., Martone, M. E., Ellisman, M. H., & Gage, F. H.
Affiliations
  • Salk Institute for Biological Studies (US)
  • University of California San Diego (US)
Corresponding author
Gage, Fred H
Venue
Nature Neuroscience
Published
2007-05-07
Volume
10
Issue
6
Pages
727-734
ISSN
  • 1097-6256 (ISSN-L)
  • 1546-1726
OpenAlex
W2061111257
Cited by
579
References
50
Citations by year
2026: 12 · 2025: 18 · 2024: 17 · 2023: 16 · 2022: 11 · 2021: 21 · 2020: 32 · 2019: 29 · 2018: 30 · 2017: 35 · 2016: 31 · 2015: 31 · 2014: 40 · 2013: 43 · 2012: 30
FWCI
13.53
Type
journal article
Language
en
Open access
closed
Locations
  • Nature Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • U.S. Public Health Service (RRP41-04050)
  • National Institute of Neurological Disorders and Stroke (R01NS050217, NS050217-02)
Topics
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
Keywords
adult neurogenesis, synaptogenesis, hippocampus, granule neurons, dendritic filopodia, dendritic spines, axospinous synapses, axodendritic synapses, axosomatic synapses, synapse maturation, neuronal connectivity
MeSH terms
Animals, Diagnostic Imaging, Female, Hippocampus, Mice, Inbred C57BL, Models, Neurological, Synaptic Transmission, Neurons, Synapses, Presynaptic Terminals, Microscopy, Confocal, Organogenesis, Microscopy, Electron, Transmission, Dendritic Spines, Mice

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