Cited by 406Developmental Biology and Gene RegulationPancreatic function and diabetesRenal and related cancers

Notochord to endoderm signaling is required for pancreas development

Kim, Seung K., Hebrok, Matthias, Melton, Douglas A.·1997·Development

Abstract

The role of the notochord in inducing and patterning adjacent neural and mesodermal tissues is well established. We provide evidence that the notochord is also required for one of the earliest known steps in the development of the pancreas, an endodermally derived organ. At a developmental stage in chick embryos when the notochord touches the endoderm, removal of notochord eliminates subsequent expression of several markers of dorsal pancreas bud development, including insulin, glucagon and carboxypeptidase A. Pancreatic gene expression can be initiated and maintained in prepancreatic chick endoderm grown in vitro with notochord. Non-pancreatic endoderm, however, does not express pancreatic genes when recombined with the same notochord. The results suggest that the notochord provides a permissive signal to endoderm to specify pan-creatic fate in a stepwise manner.

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Crossref · 10.1242/dev.124.21.4243

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Authors
Kim, S. K., Hebrok, M., & Melton, D. A.
Affiliations
Howard Hughes Medical Institute (US)
Venue
Development
Published
1997-11-01
Volume
124
Issue
21
Pages
4243-4252
ISSN
  • 0950-1991 (ISSN-L)
  • 1477-9129
OpenAlex
W1844743435
Cited by
405
References
59
Citations by year
2026: 1 · 2025: 5 · 2024: 3 · 2023: 8 · 2022: 1 · 2021: 4 · 2020: 6 · 2019: 4 · 2018: 10 · 2017: 7 · 2016: 9 · 2015: 9 · 2014: 7 · 2013: 12 · 2012: 14
FWCI
6.52
Type
journal article
Language
en
Open access
closed
Locations
  • Development (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Renal and related cancers
Keywords
notochord, endoderm, pancreatic development, dorsal pancreas, insulin, glucagon, carboxypeptidase A, chick embryo, in vitro recombination
MeSH terms
Animals, Cells, Cultured, Chick Embryo, Embryonic Induction, Endoderm, Nerve Tissue Proteins, Notochord, Pancreas, Transcription Factors, Signal Transduction, Biomarkers, Trans-Activators, Homeodomain Proteins, Gene Expression Regulation, Developmental, LIM-Homeodomain Proteins

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