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Progressive lineage analysis by cell sorting and culture identifies FLK1+VEcadherin+ cells at a diverging point of endothelial and hemopoietic lineages
Abstract
Totipotent murine ES cells h ave an enormous potential for the study of cell specification. He re we demonstrate that ES cells can diffe rentiate to hemopoietic cells th rough the proximal lateral mesoderm, me rely upon culturing in type IV collagen-coated dishes. Separation of the Flk1+ mesoderm f rom other cell lineages was critical for hemopoietic cell diffe rentiation, whe reas formation of the embryoid body was not. Since the two-dimensionally spreading cells can be monito red easily in real time, this culture system will g reatly facilitate the study of the mechanisms i nvolved in the cell specification to mesoderm, endothelial, and hemopoietic cells. In the cultu re of ES cells, how ever, lineages and stages of diffe rentiating cells can only be defined by their own characteristics. We showed that a combination of monoclonal antibodies against E-cadherin, Flk1/KDR, PDGF recepto rα, VEcadherin, CD45 and Ter119 was suf ficient to define most intermediate stages during diffe rentiation of ES cells to blood cells. Using this cultu re system and surface markers, we determined the following order for blood cell differentiation: ES cell (E-cadherin+Flk1™PDGFRα™), proximal lateral mesoderm (E-cadherin™ Flk1+VEcadherin™ ), progenitor with hemoangiogenic potential (Flk1+VE-cadherin+CD45™ ), hemopoietic p rogenitor (CD45+c-Kit+) and mature blood cells (c-Kit™ CD45+ or Ter119+), though direct differentiation of blood cells f rom the Flk1+VE-cadherin™ stage cannot be ruled out. Not only the VE-cadherin+CD45™ population generated f rom ES cells but also those di rectly sorted f rom the yolk sac of 9.5 dpc embryos h ave a potential to give rise to hemopoietic cells. P rogenitors with hemoangiogenic potential were identified in both the Flk1+VE-cadherin™and Flk1+VEcadherin+populations by the single cell deposition experiment. This line of evidence implicates Flk1+VEcadherin+ cells as a diverging point of hemopoietic and endothelial cell lineages.
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Cites
Doetschman, T. C., Eistetter, H., Katz, M., Schmidt, W., & Kemler, R. (1985) · Development · doi:10.1242/dev.87.1.27 · Cited by 1,962
Millauer, B., Wizigmann-Voos, S., Schnürch, H., Martinez, R., Møller, N. P. H., Risau, W., & Ullrich, A. (1993) · Cell · doi:10.1016/0092-8674(93)90573-9 · Cited by 1,927
Definitive Hematopoiesis Is Autonomously Initiated by the AGM Region
Medvinsky, A., & Dzierzak, E. (1996) · Cell · doi:10.1016/s0092-8674(00)80165-8 · Cited by 1,548
Risau, W., & Flamme, I. (1995) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cb.11.110195.000445 · Cited by 1,406
Generation of Lymphohematopoietic Cells from Embryonic Stem Cells in Culture
Nakano, T., Kodama, H., & Honjo, T. (1994) · Science · doi:10.1126/science.8066449 · Cited by 871
Self-citationGerm-line transmission of genes introduced into cultured pluripotential cells by retroviral vector
Robertson, E., Bradley, A., Kuehn, M., & Evans, M. (1986) · Nature · doi:10.1038/323445a0 · Cited by 751
Expression and function of c-kit in hemopoietic progenitor cells.
Ogawa, M., Matsuzaki, Y., Nishikawa, S., Hayashi, S., Kunisada, T., Sudo, T., Kina, T., Nakauchi, H., & Nishikawa, S. (1991) · The Journal of experimental medicine · doi:10.1084/jem.174.1.63 · Cited by 743
Self-citationflk-1, an flt-related receptor tyrosine kinase is an early marker for endothelial cell precursors
Yamaguchi, T. P., Dumont, D. J., Conlon, R. A., Breitman, M. L., & Rossant, J. (1993) · Development · doi:10.1242/dev.118.2.489 · Cited by 695
Multiple hematopoietic lineages develop from embryonic stem (ES) cells in culture
Wiles, M. V., & Keller, G. (1991) · Development · doi:10.1242/dev.111.2.259 · Cited by 596
Dumont, D. J., Fong, G., Puri, M. C., Gradwohl, G., Alitalo, K., & Breitman, M. L. (1995) · Developmental Dynamics · doi:10.1002/aja.1002030109 · Cited by 539
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Klein, A. M., Mazutis, L., Akartuna, I., Tallapragada, N., Veres, A., Li, V., Peshkin, L., Weitz, D. A., & Kirschner, M. W. (2015) · Cell · doi:10.1016/j.cell.2015.04.044 · Cited by 3,356
Transplantation of ex vivo expanded endothelial progenitor cells for therapeutic neovascularization
Kalka, C., Masuda, H., Takahashi, T., Kalka-Moll, W. M., Silver, M., Kearney, M., Li, T., Isner, J. M., & Asahara, T. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.7.3422 · Cited by 1,863
Flk1-positive cells derived from embryonic stem cells serve as vascular progenitors
Yamashita, J., Itoh, H., Hirashima, M., Ogawa, M., Nishikawa, S., Yurugi, T., Naito, M., Nakao, K., & Nishikawa, S.-I. (2000) · Nature · doi:10.1038/35040568 · Cited by 1,348
Self-citationInduction of Midbrain Dopaminergic Neurons from ES Cells by Stromal Cell–Derived Inducing Activity
Kawasaki, H., Mizuseki, K., Nishikawa, S., Kaneko, S., Kuwana, Y., Nakanishi, S., Nishikawa, S.-I., & Sasai, Y. (2000) · Neuron · doi:10.1016/s0896-6273(00)00083-0 · Cited by 1,277
Self-citationEmbryonic stem cell differentiation: emergence of a new era in biology and medicine
Keller, G. (2005) · Genes & Development · doi:10.1101/gad.1303605 · Cited by 1,151
Visualizing structure and transitions in high-dimensional biological data
Moon, K. R., van Dijk, D., Wang, Z., Gigante, S., Burkhardt, D. B., Chen, W. S., Yim, K., Elzen, A. V. D., Hirn, M. J., Coifman, R. R., et al. (2019) · Nature Biotechnology · doi:10.1038/s41587-019-0336-3 · Cited by 1,065
Origin of endothelial progenitors in human postnatal bone marrow
Reyes, M., Dudek, A., Jahagirdar, B., Koodie, L., Marker, P. H., & Verfaillie, C. M. (2002) · Journal of Clinical Investigation · doi:10.1172/jci0214327 · Cited by 887
Generation of Pluripotent Stem Cells from Neonatal Mouse Testis
Kanatsu-Shinohara, M., Inoue, K., Lee, J., Yoshimoto, M., Ogonuki, N., Miki, H., Baba, S., Kato, T., Kazuki, Y., Toyokuni, S., et al. (2004) · Cell · doi:10.1016/j.cell.2004.11.011 · Cited by 814
Kunath, T., Saba-El-Leil, M. K., Almousailleakh, M., Wray, J., Meloche, S., & Smith, A. (2007) · Development · doi:10.1242/dev.02880 · Cited by 801
Embryonic Stem Cells: Prospects for Developmental Biology and Cell Therapy
Wobus, A. M., & Boheler, K. R. (2005) · Physiological Reviews · doi:10.1152/physrev.00054.2003 · Cited by 782
Cao, Y., Sun, Z., Liao, L., Meng, Y., Han, Q., & Zhao, R. C. (2005) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2005.04.135 · Cited by 729
Kattman, S. J., Huber, T. L., & Keller, G. M. (2006) · Developmental Cell · doi:10.1016/j.devcel.2006.10.002 · Cited by 729
Cbfa2 is required for the formation of intra-aortic hematopoietic clusters
North, T., Gu, T.-L., Stacy, T., Wang, Q., Howard, L., Binder, M., Marín-Padilla, M., & Speck, N. A. (1999) · Development · doi:10.1242/dev.126.11.2563 · Cited by 662
de Bruijn, M. F. T. R. (2000) · The EMBO Journal · doi:10.1093/emboj/19.11.2465 · Cited by 616
Diversification of haematopoietic stem cells to specific lineages
Orkin, S. H. (2000) · Nature Reviews Genetics · doi:10.1038/35049577 · Cited by 565
VEGF inhibits T-cell development and may contribute to tumor-induced immune suppression
Ohm, J. E., Gabrilovich, D. I., Sempowski, G. D., Kisseleva, E., Parman, K. S., Nadaf, S., & Carbone, D. P. (2003) · Blood · doi:10.1182/blood-2002-07-1956 · Cited by 538
Identification and characterization of subpopulations in undifferentiated ES cell culture
Toyooka, Y., Shimosato, D., Murakami, K., Takahashi, K., & Niwa, H. (2008) · Development · doi:10.1242/dev.017400 · Cited by 530
Fazel, S. (2006) · Journal of Clinical Investigation · doi:10.1172/jci27019 · Cited by 517
Kennedy, M., D'Souza, S. L., Lynch-Kattman, M., Schwantz, S., & Keller, G. (2007) · Blood · doi:10.1182/blood-2006-09-047704 · Cited by 483
Tada, S., Era, T., Furusawa, C., Sakurai, H., Nishikawa, S., Kinoshita, M., Nakao, K., Chiba, T., & Nishikawa, S.-I. (2005) · Development · doi:10.1242/dev.02005 · Cited by 455
Self-citationOntogeny of the Hematopoietic System
Cumano, A., & Godin, I. (2007) · Annual Review of Immunology · doi:10.1146/annurev.immunol.25.022106.141538 · Cited by 434
Roles of TGF-β family signaling in stem cell renewal and differentiation
Watabe, T., & Miyazono, K. (2009) · Cell Research · doi:10.1038/cr.2008.323 · Cited by 428
Embryonic origin of the adult hematopoietic system: advances and questions
Medvinsky, A., Rybtsov, S., & Taoudi, S. (2011) · Development · doi:10.1242/dev.040998 · Cited by 403
Kinder, S. J., Tsang, T. E., Quinlan, G. A., Hadjantonakis, A.-K., Nagy, A., & Tam, P. P. L. (1999) · Development · doi:10.1242/dev.126.21.4691 · Cited by 403
Fetal monocytes and the origins of tissue-resident macrophages
Hoeffel, G., & Ginhoux, F. (2018) · Cellular Immunology · doi:10.1016/j.cellimm.2018.01.001 · Cited by 401
The end of the beginning for pluripotent stem cells
Donovan, P. J., & Gearhart, J. (2001) · Nature · doi:10.1038/35102154 · Cited by 335
CD41 expression defines the onset of primitive and definitive hematopoiesis in the murine embryo
Ferkowicz, M. J., Starr, M., Xie, X., Li, W., Johnson, S. A., Shelley, W. C., Morrison, P. R., & Yoder, M. C. (2003) · Development · doi:10.1242/dev.00632 · Cited by 333
Role of vascular endothelial-cadherin in vascular morphogenesis
Gory-Fauré, S., Prandini, M.-H., Pointu, H., Roullot, V., Pignot-Paintrand, I., Vernet, M., & Huber, P. (1999) · Development · doi:10.1242/dev.126.10.2093 · Cited by 317
Yoshida, H., Honda, K., Shinkura, R., Adachi, S., Nishikawa, S., Maki, K., Ikuta, K., & Nishikawa, S.-I. (1999) · International Immunology · doi:10.1093/intimm/11.5.643 · Cited by 287
Self-citationMaturation of Embryonic Stem Cells Into Endothelial Cells in an In Vitro Model of Vasculogenesis
Hirashima, M., Kataoka, H., Nishikawa, S., Matsuyoshi, N., & Nishikawa, S.-I. (1999) · Blood · doi:10.1182/blood.v93.4.1253 · Cited by 276
Self-citationNotch Promotes Neural Lineage Entry by Pluripotent Embryonic Stem Cells
Lowell, S., Benchoua, A., Heavey, B., & Smith, A. G. (2006) · PLoS Biology · doi:10.1371/journal.pbio.0040121 · Cited by 260
Göttgens, B., Nastos, A., Kinston, S., Piltz, S., Delabesse, E. C. M., Stanley, M., Sanchez, M., Ciau‐Uitz, A., Patient, R., & Green, A. R. (2002) · The EMBO Journal · doi:10.1093/emboj/cdf286 · Cited by 212
Nakamura, T., Torimura, T., Sakamoto, M., Hashimoto, O., Taniguchi, E., Inoue, K., Sakata, R., Kumashiro, R., Murohara, T., Ueno, T., et al. (2007) · Gastroenterology · doi:10.1053/j.gastro.2007.03.110 · Cited by 156
Anterior definitive endoderm from ESCs reveals a role for FGF signaling
Morrison, G. M., Oikonomopoulou, I., Migueles, R. P., Soneji, S., Livigni, A., Enver, T., & Brickman, J. M. (2008) · Cell Stem Cell · doi:10.1016/j.stem.2008.07.021 · Cited by 121
Stem cell sources for vascular tissue engineering and regeneration
Bajpai, V. K., & Andreadis, S. T. (2012) · Tissue Engineering Part B: Reviews · doi:10.1089/ten.teb.2011.0264 · Cited by 95
Mukouyama, Y.-S., Chiba, N., Hara, T., Okada, H., Ito, Y., Kanamaru, R., Miyajima, A., Satake, M., & Watanabe, T. (2000) · Developmental Biology · doi:10.1006/dbio.2000.9617 · Cited by 81
Differentiation Patterns of Embryonic Stem Cells in Two- versus Three-Dimensional Culture
Pineda, E. T., Nerem, R. M., & Ahsan, T. (2013) · Cells Tissues Organs · doi:10.1159/000346166 · Cited by 77
The embryonic origins of hematopoietic stem cells: a tale of hemangioblast and hemogenic endothelium
BOLLEROT, K., POUGET, C., & JAFFREDO, T. (2005) · APMIS · doi:10.1111/j.1600-0463.2005.apm_317.x · Cited by 65
Takebe, A., Era, T., Okada, M., Martin Jakt, L., Kuroda, Y., & Nishikawa, S.-I. (2006) · Developmental Biology · doi:10.1016/j.ydbio.2005.12.016 · Cited by 54
Self-citationWareing, S., Eliades, A., Lacaud, G., & Kouskoff, V. (2012) · Developmental Dynamics · doi:10.1002/dvdy.23825 · Cited by 46
Metadata
- Authors
- Nishikawa, S.-I., Nishikawa, S., Hirashima, M., Matsuyoshi, N., & Kodama, H.
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 1998-05-01
- Volume
- 125
- Issue
- 9
- Pages
- 1747-1757
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W2139961479
- Cited by
- 829
- References
- 40
- FWCI
- 9.34
- Type
- journal article
- Language
- en
- Open access
- closed
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