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Apoptosis Inducing Activity of Fucoidan in HCT-15 Colon Carcinoma Cells
Open accessAbstract
The antitumor activity of fucoidan from Fucus vesiculosus was investigated in human colon carcinoma cells. The crude fucoidan, a polysaccharide composed predominantly of sulfated fucose, markedly inhibited the growth of HCT-15 cells (human colon carcinoma cells). After HCT-15 cells were treated with fucoidan, several apoptotic events such as DNA fragmentation, chromatin condensation and increase of the population of sub-G1 hypodiploid cells were observed. In the mechanism of fucoidan-induced apoptosis, we examined changes in Bcl-2 and Bax protein expression levels and activation of caspases. Fucoidan decreased Bcl-2 expression, whereas the expression of Bax was increased in a time-dependent manner compared to the control. In addition, the active forms of caspase-9 and caspase-3 were increased, and the cleavage of poly(ADP-ribose) polymerase (PARP), a vital substrate of effector caspase, was observed. Furthermore, the induction of apoptosis was also accompanied by a strong activation of extracellular signal-regulated kinase (ERK) and p38 kinase and an inactivation of phosphatidylinositol 3-kinase (PI3K)/Akt in a time-dependent manner. These findings provide evidence demonstrating that the pro-apoptotic effect of fucoidan is mediated through the activation of ERK, p38 and the blocking of the PI3K/Akt signal pathway in HCT-15 cells. These data support the hypothesis that fucoidan may have potential in colon cancer treatment.
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“Fucoidans were shown to induce apoptosis of some other cancer cells, for example HT-29, HCT116, and HCT-15 human colon cancer cells [42,43] as well as MCF-7 (breast adenocarcinoma) [44], melanoma SK-Mel-28, breast cancer T-47D [45], and human promyeloid leukemic cell lines [46].”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [42]
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“Another group reported that the pro-apoptotic effect of fucoidan from F. vesiculosus was mediated by the activation of ERKs, p38 and by the blocking of PI3K/Akt signaling pathway in HCT-15 cells [42].”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [42]
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Yu, Y., Shen, M., Song, Q., & Xie, J. (2017) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2017.12.009 · Cited by 1,447
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Metadata
- Authors
- Hyun, J.-H., Kim, S.-C., Kang, J.-I., Kim, M.-K., Boo, H.-J., Kwon, J.-M., Koh, Y.-S., Hyun, J.-W., Park, D.-B., Yoo, E.-S., & Kang, H.-K.
- Affiliations
- Jeju National University (KR)
- School of Medicine, Institute of Medical Sciences, Jeju National University
- Venue
- Biological & Pharmaceutical Bulletin
- Publisher
- Pharmaceutical Society of Japan
- Published
- 2009
- Volume
- 32
- Issue
- 10
- Pages
- 1760-1764
- ISSN
- 0918-6158 (ISSN-L)
- 1347-5215
- OpenAlex
- W2084350967
- Cited by
- 150
- References
- 35
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 3 · 2023: 4 · 2022: 5 · 2021: 13 · 2020: 8 · 2019: 14 · 2018: 15 · 2017: 11 · 2016: 10 · 2015: 11 · 2014: 11 · 2013: 16 · 2012: 5
- FWCI
- 11.07
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Biological and Pharmaceutical Bulletin (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Jeju National University
- Jeju National University Hospital
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Echinoderm biology and ecology
- Keywords
- fucoidan, Fucus vesiculosus, apoptosis, colon cancer, HCT-15 cells, Bcl-2, Bax, caspase-9, caspase-3, PARP cleavage, ERK, p38 MAPK, PI3K/Akt signaling, anticancer activity
- MeSH terms
- Adenocarcinoma, Antineoplastic Agents, Phytogenic, Colonic Neoplasms, Humans, Phytotherapy, Plant Extracts, Poly(ADP-ribose) Polymerases, Polysaccharides, Signal Transduction, Apoptosis, Proto-Oncogene Proteins c-bcl-2, Phosphatidylinositol 3-Kinases, Fucus, Cell Line, Tumor, Extracellular Signal-Regulated MAP Kinases, p38 Mitogen-Activated Protein Kinases, bcl-2-Associated X Protein, Proto-Oncogene Proteins c-akt, Caspase 3, Caspase 9
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