Fucoidan induces apoptosis of human HS-Sultan cells accompanied by activation of caspase-3 and down-regulation of ERK Pathways
Abstract
Fucoidan, a sulfated polysaccharide in brown seaweed, was found to inhibit proliferation and induce apoptosis in human lymphoma HS-Sultan cell lines. Fucoidan-induced apoptosis was accompanied by the activation of caspase-3 and was partially prevented by pretreatment with a pan-caspase inhibitor, z-VAD-FMK. The mitochondrial potential in HS-Sultan cells was decreased 24 hr after treatment with fucoidan, indicating that fucoidan induced apoptosis through a mitochondrial pathway. When HS-Sultan was treated with 100 microg/mL fucoidan for 24 hr, phosphorylation of ERK and GSK markedly decreased. In contrast, phosphorylation of p38 and Akt was not altered by treatment with fucoidan. L-selectin and P-selectin are known to be receptors of fucoidan; however, as HS-Sultan does not express either of these selectins, it is unlikely that fucoidan induced apoptosis through them in HS-Sultan. The neutralizing antibody, Dreg56, against human L-selectin did not prevent the inhibitory effect of fucoidan on the proliferation of IM9 and MOLT4 cells, both of which express L-selectin; thus it is possible fucoidan induced apoptosis though different receptors. These results demonstrate that fucoidan has direct anti-cancer effects on human HS-Sultan cells through caspase and ERK pathways.
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The effect of fucoidan on human lymphoma HS-Sultan cells is explained by activation of caspase-3 and down regulation of the kinase pathway.
“This effect was explained by the consequent activation of caspase-3 and down regulation of the kinase pathway [179].”
From Marine Natural Products: A Source of Novel Anticancer Drugs (2019) · cites this paper as [179]
Fucoidan from F. vesiculosus decreased phosphorylation of ERKs but not p38.
“The fucoidan from F. vesiculosus clearly decreased the phosphorylation of ERKs but not p38 [47].”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [47]
Fucoidan induces apoptosis in HT-29 colon cancer cells, MCF-7 human breast cancer cells, and HS-Sultan human lymphoma cells.
“Fucoidan was also reported to induce apoptosis in HT-29 colon cancer cells, 47 MCF-7 human breast cancer cells, 48 and HS-Sultan human lymphoma cells.49”
From Fucoidans from brown seaweeds: an update on structures, extraction techniques and use of enzymes as tools for structural elucidation (2013) · cites this paper as [49]
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- Authors
- Aisa, Y., Miyakawa, Y., Nakazato, T., Shibata, H., Saito, K., Ikeda, Y., & Kizaki, M.
- Affiliations
- Keio University (JP)
- Yakult Central Institute (JP)
- Corresponding author
- Miyakawa, Yoshitaka
- Venue
- American Journal of Hematology
- Publisher
- Wiley
- Published
- 2004
- Volume
- 78
- Issue
- 1
- Pages
- 7-14
- ISSN
- 0361-8609 (ISSN-L)
- 1096-8652
- OpenAlex
- W2109148114
- Cited by
- 307
- References
- 1
- Citations by year
- 2026: 4 · 2025: 6 · 2024: 9 · 2023: 10 · 2022: 10 · 2021: 9 · 2020: 13 · 2019: 22 · 2018: 20 · 2017: 15 · 2016: 19 · 2015: 22 · 2014: 28 · 2013: 29 · 2012: 16
- FWCI
- 5.81
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Hematology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Natural product bioactivities and synthesis
- Keywords
- fucoidan, apoptosis, caspase-3, ERK signaling, lymphoma, mitochondrial pathway, L-selectin, P-selectin, sulfated polysaccharides, anticancer effects, cell proliferation inhibition, z-VAD-fmk
- MeSH terms
- Antibodies, Antineoplastic Agents, Enzyme Activation, Humans, Leukemia, Lymphoma, Multiple Myeloma, Polysaccharides, Signal Transduction, Down-Regulation, Apoptosis, Lithium Chloride, P-Selectin, L-Selectin, Caspases, Glycogen Synthase Kinases, Cell Line, Tumor, Extracellular Signal-Regulated MAP Kinases, Caspase 3
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