Cited by 307Seaweed-derived Bioactive CompoundsCell death mechanisms and regulationNatural product bioactivities and synthesis

Fucoidan induces apoptosis of human HS-Sultan cells accompanied by activation of caspase-3 and down-regulation of ERK Pathways

Aisa, Yoshinobu, Miyakawa, Yoshitaka, Nakazato, Tomonori, Shibata, Hideyuki, Saito, Kaori, Ikeda, Yasuo, Kizaki, Masahiro·2005·American Journal of Hematology

Abstract

Fucoidan, a sulfated polysaccharide in brown seaweed, was found to inhibit proliferation and induce apoptosis in human lymphoma HS-Sultan cell lines. Fucoidan-induced apoptosis was accompanied by the activation of caspase-3 and was partially prevented by pretreatment with a pan-caspase inhibitor, z-VAD-FMK. The mitochondrial potential in HS-Sultan cells was decreased 24 hr after treatment with fucoidan, indicating that fucoidan induced apoptosis through a mitochondrial pathway. When HS-Sultan was treated with 100 microg/mL fucoidan for 24 hr, phosphorylation of ERK and GSK markedly decreased. In contrast, phosphorylation of p38 and Akt was not altered by treatment with fucoidan. L-selectin and P-selectin are known to be receptors of fucoidan; however, as HS-Sultan does not express either of these selectins, it is unlikely that fucoidan induced apoptosis through them in HS-Sultan. The neutralizing antibody, Dreg56, against human L-selectin did not prevent the inhibitory effect of fucoidan on the proliferation of IM9 and MOLT4 cells, both of which express L-selectin; thus it is possible fucoidan induced apoptosis though different receptors. These results demonstrate that fucoidan has direct anti-cancer effects on human HS-Sultan cells through caspase and ERK pathways.

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Crossref · 10.1002/ajh.20182

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Authors
Aisa, Y., Miyakawa, Y., Nakazato, T., Shibata, H., Saito, K., Ikeda, Y., & Kizaki, M.
Affiliations
  • Keio University (JP)
  • Yakult Central Institute (JP)
Corresponding author
Miyakawa, Yoshitaka
Venue
American Journal of Hematology
Publisher
Wiley
Published
2004
Volume
78
Issue
1
Pages
7-14
ISSN
  • 0361-8609 (ISSN-L)
  • 1096-8652
OpenAlex
W2109148114
Cited by
307
References
1
Citations by year
2026: 4 · 2025: 6 · 2024: 9 · 2023: 10 · 2022: 10 · 2021: 9 · 2020: 13 · 2019: 22 · 2018: 20 · 2017: 15 · 2016: 19 · 2015: 22 · 2014: 28 · 2013: 29 · 2012: 16
FWCI
5.81
Type
journal article
Language
en
Open access
closed
Locations
  • American Journal of Hematology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Natural product bioactivities and synthesis
Keywords
fucoidan, apoptosis, caspase-3, ERK signaling, lymphoma, mitochondrial pathway, L-selectin, P-selectin, sulfated polysaccharides, anticancer effects, cell proliferation inhibition, z-VAD-fmk
MeSH terms
Antibodies, Antineoplastic Agents, Enzyme Activation, Humans, Leukemia, Lymphoma, Multiple Myeloma, Polysaccharides, Signal Transduction, Down-Regulation, Apoptosis, Lithium Chloride, P-Selectin, L-Selectin, Caspases, Glycogen Synthase Kinases, Cell Line, Tumor, Extracellular Signal-Regulated MAP Kinases, Caspase 3

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