The DOI matches its Crossref record; 13 of the 13 references it cites were found in a registry or an index.
Processing of Pre-microRNAs by the Dicer-1–Loquacious Complex in Drosophila Cells
Open accessAbstract
microRNAs (miRNAs) are a large family of 21- to 22-nucleotide non-coding RNAs that interact with target mRNAs at specific sites to induce cleavage of the message or inhibit translation. miRNAs are excised in a stepwise process from primary miRNA (pri-miRNA) transcripts. The Drosha-Pasha/DGCR8 complex in the nucleus cleaves pri-miRNAs to release hairpin-shaped precursor miRNAs (pre-miRNAs). These pre-miRNAs are then exported to the cytoplasm and further processed by Dicer to mature miRNAs. Here we show that Drosophila Dicer-1 interacts with Loquacious, a double-stranded RNA-binding domain protein. Depletion of Loquacious results in pre-miRNA accumulation in Drosophila S2 cells, as is the case for depletion of Dicer-1. Immuno-affinity purification experiments revealed that along with Dicer-1, Loquacious resides in a functional pre-miRNA processing complex, and stimulates and directs the specific pre-miRNA processing activity. These results support a model in which Loquacious mediates miRNA biogenesis and, thereby, the expression of genes regulated by miRNAs.
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Cited by
Regulation of microRNA biogenesis
Ha, M., & Kim, V. N. (2014) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3838 · Cited by 5,462
Biogenesis of small RNAs in animals
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Human RISC Couples MicroRNA Biogenesis and Posttranscriptional Gene Silencing
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Metadata
- Authors
- Saito, K., Ishizuka, A., Siomi, H., & Siomi, M. C.
- Venue
- PLoS Biology
- Publisher
- Public Library of Science (PLoS)
- Published
- 2005-05-24
- Volume
- 3
- Issue
- 7
- Pages
- e235
- ISSN
- 1544-9173 (ISSN-L)
- 1545-7885
- OpenAlex
- W2135066895
- Cited by
- 440
- References
- 13
- FWCI
- 14.08
- Type
- journal article
- Language
- en
- Open access
- gold
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- Saito, K., Ishizuka, A., Siomi, H., & Siomi, M. C. (2005). Processing of Pre-microRNAs by the Dicer-1–Loquacious Complex in Drosophila Cells. PLoS Biology. https://doi.org/10.1371/journal.pbio.0030235
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