Cited by 97Developmental Biology and Gene RegulationNeurogenesis and neuroplasticity mechanismsCongenital gastrointestinal and neural anomalies

Identification of multiple subsets of ventral interneurons and differential distribution along the rostrocaudal axis of the developing spinal cord

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Francius, Cédric, Harris, Audrey, Rucchin, Vincent, Hendricks, Timothy J., Stam, Floor J., Barber, Melissa, Kurek, Dorota, Grosveld, Frank G. +3 more·2013·PLoS ONE

Abstract

The spinal cord contains neuronal circuits termed Central Pattern Generators (CPGs) that coordinate rhythmic motor activities. CPG circuits consist of motor neurons and multiple interneuron cell types, many of which are derived from four distinct cardinal classes of ventral interneurons, called V0, V1, V2 and V3. While significant progress has been made on elucidating the molecular and genetic mechanisms that control ventral interneuron differentiation, little is known about their distribution along the antero-posterior axis of the spinal cord and their diversification. Here, we report that V0, V1 and V2 interneurons exhibit distinct organizational patterns at brachial, thoracic and lumbar levels of the developing spinal cord. In addition, we demonstrate that each cardinal class of ventral interneurons can be subdivided into several subsets according to the combinatorial expression of different sets of transcription factors, and that these subsets are differentially distributed along the rostrocaudal axis of the spinal cord. This comprehensive molecular profiling of ventral interneurons provides an important resource for investigating neuronal diversification in the developing spinal cord and for understanding the contribution of specific interneuron subsets on CPG circuits and motor control.

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  1. [31]Jankowska E (2001) Spinal interneuronal systems: identification, multifunctional character and reconfigurations in mammals. The Journal of physiology 533: 31– 40. 11351010 PMC2278593

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Authors
Francius, C., Harris, A., Rucchin, V., Hendricks, T. J., Stam, F. J., Barber, M., Kurek, D., Grosveld, F. G., Pierani, A., Goulding, M., & Clotman, F.
Affiliations
  • UCLouvain (BE)
  • Salk Institute for Biological Studies (US)
  • Centre National de la Recherche Scientifique (FR)
  • Erasmus MC (NL)
Corresponding author
Clotman, Frédéric
Venue
PLoS ONE
Published
2013-08-15
Volume
8
Issue
8
Pages
e70325
ISSN
1932-6203 (ISSN-L)
OpenAlex
W2139190679
Cited by
97
References
132
Citations by year
2026: 7 · 2025: 3 · 2024: 11 · 2023: 10 · 2022: 8 · 2021: 7 · 2020: 3 · 2019: 14 · 2018: 10 · 2017: 5 · 2016: 6 · 2015: 10 · 2014: 3
FWCI
2.83
Type
journal article
Language
en
Open access
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, other-oa)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • EUR Research Repository (Erasmus University Rotterdam) (submittedVersion, other-oa)
  • RePub (Erasmus University Rotterdam) (submittedVersion, other-oa)
  • Digital Access to Libraries (Université catholique de Louvain (UCL), l'Université de Namur (UNamur) and the Université Saint-Louis (USL-B)) (submittedVersion, other-oa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Figshare (submittedVersion, cc-by)
Funders
  • Association Belge contre les Maladies Neuro-Musculaires
  • Fonds De La Recherche Scientifique - FNRS
  • Centre National de la Recherche Scientifique
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Health Sciences › Medicine › Surgery › Congenital gastrointestinal and neural anomalies
Keywords
spinal cord development, rostrocaudal axis, central pattern generator, transcription factors, motor control, molecular profiling
MeSH terms
Animals, Anterior Horn Cells, Cell Differentiation, Cell Movement, Interneurons, Spinal Cord, Mice, Knockout, Mice

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