Identification of multiple subsets of ventral interneurons and differential distribution along the rostrocaudal axis of the developing spinal cord
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The spinal cord contains neuronal circuits termed Central Pattern Generators (CPGs) that coordinate rhythmic motor activities. CPG circuits consist of motor neurons and multiple interneuron cell types, many of which are derived from four distinct cardinal classes of ventral interneurons, called V0, V1, V2 and V3. While significant progress has been made on elucidating the molecular and genetic mechanisms that control ventral interneuron differentiation, little is known about their distribution along the antero-posterior axis of the spinal cord and their diversification. Here, we report that V0, V1 and V2 interneurons exhibit distinct organizational patterns at brachial, thoracic and lumbar levels of the developing spinal cord. In addition, we demonstrate that each cardinal class of ventral interneurons can be subdivided into several subsets according to the combinatorial expression of different sets of transcription factors, and that these subsets are differentially distributed along the rostrocaudal axis of the spinal cord. This comprehensive molecular profiling of ventral interneurons provides an important resource for investigating neuronal diversification in the developing spinal cord and for understanding the contribution of specific interneuron subsets on CPG circuits and motor control.
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Pou3f1, Onecut2 and Maf also demarcated subpopulations of multiple neuronal classes.
“Furthermore, Pou3f1, Onecut2 and Maf also demarcated subpopulations of multiple neuronal classes, which is consistent with the expression of these TFs in multiple types of neurons (Francius et al., 2013).”
From Single cell transcriptomics reveals spatial and temporal dynamics of gene expression in the developing mouse spinal cord (2019) · cites this paper as Francius et al., 2013
The lateral V2a subgroup diversifies further into subgroups labelled by Shox2 and Maf/onecut family TFs.
“Furthermore, the lateral subgroup appears to diversify further into subgroups that are labelled by Shox2 and Maf/onecut family TFs (Francius et al., 2013; Harris et al., 2018 preprint).”
From Single cell transcriptomics reveals spatial and temporal dynamics of gene expression in the developing mouse spinal cord (2019) · cites this paper as Francius et al., 2013
V3 interneurons have been previously shown to comprise at least two subtypes with distinct properties and settling positions in the spinal cord.
“V3 interneurons have been previously shown to comprise at least two subtypes with distinct properties and settling positions in the spinal cord”
From Single cell transcriptomics reveals spatial and temporal dynamics of gene expression in the developing mouse spinal cord (2019) · cites this paper as Francius et al., 2013
The 11 neural progenitor domains (p0-p3, pMN, pd1-pd6) generate distinct classes of spinal neurons that further diversify into multiple subpopulations.
“The 11 neural progenitor domains (p0-p3, pMN, pd1-pd6) generate distinct classes of spinal neurons that further diversify into multiple subpopulations”
From Single cell transcriptomics reveals spatial and temporal dynamics of gene expression in the developing mouse spinal cord (2019) · cites this paper as Francius et al., 2013
Pou3f1 had previously been reported in multiple neuronal subtypes of the spinal cord, e.g. in MNs, V2a and V3 interneurons, which the analysis correctly identified.
“This TF had previously been reported in multiple neuronal subtypes of the spinal cord, e.g. in MNs, V2a and V3 interneurons (Dasen et al., 2005; Francius et al., 2013), which we correctly identified.”
From Single cell transcriptomics reveals spatial and temporal dynamics of gene expression in the developing mouse spinal cord (2019) · cites this paper as Francius et al., 2013
In e12.5 mice, dorsal V3d neurons express onecut family TFs, whereas ventral V3v neurons express Olig3.
“In e12.5 mice, dorsal V3d neurons express onecut family TFs, whereas ventral V3v neurons express Olig3 (Francius et al., 2013).”
From Single cell transcriptomics reveals spatial and temporal dynamics of gene expression in the developing mouse spinal cord (2019) · cites this paper as Francius et al., 2013
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- Authors
- Francius, C., Harris, A., Rucchin, V., Hendricks, T. J., Stam, F. J., Barber, M., Kurek, D., Grosveld, F. G., Pierani, A., Goulding, M., & Clotman, F.
- Affiliations
- UCLouvain (BE)
- Salk Institute for Biological Studies (US)
- Centre National de la Recherche Scientifique (FR)
- Erasmus MC (NL)
- Corresponding author
- Clotman, Frédéric
- ORCID
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2013-08-15
- Volume
- 8
- Issue
- 8
- Pages
- e70325
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC3744532
- OpenAlex
- W2139190679
- Cited by
- 97
- References
- 132
- Citations by year
- 2026: 7 · 2025: 3 · 2024: 11 · 2023: 10 · 2022: 8 · 2021: 7 · 2020: 3 · 2019: 14 · 2018: 10 · 2017: 5 · 2016: 6 · 2015: 10 · 2014: 3
- FWCI
- 2.83
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, other-oa)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- EUR Research Repository (Erasmus University Rotterdam) (submittedVersion, other-oa)
- RePub (Erasmus University Rotterdam) (submittedVersion, other-oa)
- Digital Access to Libraries (Université catholique de Louvain (UCL), l'Université de Namur (UNamur) and the Université Saint-Louis (USL-B)) (submittedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Figshare (submittedVersion, cc-by)
- Funders
- Association Belge contre les Maladies Neuro-Musculaires
- Fonds De La Recherche Scientifique - FNRS
- Centre National de la Recherche Scientifique
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Health Sciences › Medicine › Surgery › Congenital gastrointestinal and neural anomalies
- Keywords
- spinal cord development, rostrocaudal axis, central pattern generator, transcription factors, motor control, molecular profiling
- MeSH terms
- Animals, Anterior Horn Cells, Cell Differentiation, Cell Movement, Interneurons, Spinal Cord, Mice, Knockout, Mice
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