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Cited by 20Advanced MRI Techniques and ApplicationsAdvanced Neuroimaging Techniques and ApplicationsIon Transport and Channel Regulation

Differential effects of aquaporin-4 channel inhibition on BOLD fMRI and diffusion fMRI responses in mouse visual cortex

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Komaki, Yuji, Debacker, Clement, Djemai, Boucif, Ciobanu, Luisa, Tsurugizawa, Tomokazu, Bihan, Denis Le·2020·PLOS ONE
DOI 10.1371/journal.pone.0228759PMC PMC7241787

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Abstract

The contribution of astrocytes to the BOLD fMRI and DfMRI responses in visual cortex of mice following visual stimulation was investigated using TGN-020, an aquaporin 4 (AQP4) channel blocker, acting as an astrocyte function perturbator. Under TGN-020 injection the amplitude of the BOLD fMRI response became significantly higher. In contrast no significant changes in the DfMRI responses and the electrophysiological responses were observed. Those results further confirm the implications of astrocytes in the neurovascular coupling mechanism underlying BOLD fMRI, but not in the DfMRI responses which remained unsensitive to astrocyte function perturbation.

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  • Diffusion responses are more spatially restricted than gradient-echo BOLD, persist when neurovascular coupling is pharmacologically suppressed while neuronal activity is preserved, and are reduced by interventions that inhibit cell swelling without substantially affecting BOLD.

    “Diffusion responses are more spatially restricted than gradient-echo BOLD, persist when neurovascular coupling is pharmacologically suppressed while neuronal activity is preserved, and are reduced by interventions that inhibit cell swelling without substantially affecting BOLD (Komaki et al., 2020).”

    From 40 Years of diffusion MRI in the brain: From history to emerging frontiers (2026) · cites this paper as Komaki et al., 2020

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Cited by

Sorted by citations

Authors
Komaki, Y., Debacker, C., Djemai, B., Ciobanu, L., Tsurugizawa, T., & Bihan, D. L.
Affiliations
Commissariat à l'Énergie Atomique et aux Énergies Alternatives (FR)
Corresponding author
Bihan, Denis Le
ORCID
Venue
PLOS ONE
Publisher
Public Library of Science (PLoS)
Published
2020-05-21
Volume
15
Issue
5
Pages
e0228759
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0228759
PMC
PMC7241787
OpenAlex
W3028421556
Cited by
20
References
3
Citations by year
2026: 2 · 2025: 3 · 2024: 3 · 2023: 3 · 2022: 3 · 2021: 4 · 2020: 2
FWCI
1.46
Type
journal article
Language
en
Update notices
correction (2020-07-14)
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Louis-Jeantet Foundation (Louis-Jeantet Award)
  • Uehara Memorial Foundation (post-doctoral followship)
Topics
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced MRI Techniques and Applications
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced Neuroimaging Techniques and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
Keywords
aquaporin-4, TGN-020, astrocytes, BOLD fMRI, visual cortex, neurovascular coupling, visual stimulation, mice
MeSH terms
Animals, Astrocytes, Brain Mapping, Male, Mice, Inbred C57BL, Niacinamide, Thiadiazoles, Visual Cortex, Diffusion Magnetic Resonance Imaging, Mice, Aquaporin 4

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