The DOI matches its Crossref record; 13 of the 13 references it cites were found in a registry or an index.
Advances in the formation, use and understanding of multi-cellular spheroids
Open accessAbstract
INTRODUCTION: Developing in vitro models for studying cell biology and cell physiology is of great importance to the fields of biotechnology, cancer research, drug discovery, toxicity testing, as well as the emerging fields of tissue engineering and regenerative medicine. Traditional two-dimensional (2D) methods of mammalian cell culture have several limitations and it is increasingly recognized that cells grown in a three-dimensional (3D) environment more closely represent normal cellular function due to the increased cell-to-cell interactions, and by mimicking the in vivo architecture of natural organs and tissues. AREAS COVERED: In this review, we discuss the methods to form 3D multi-cellular spheroids, the advantages and limitations of these methods, and assays used to characterize the function of spheroids. The use of spheroids has led to many advances in basic cell sciences, including understanding cancer cell interactions, creating models for drug discovery and cancer metastasis, and they are being investigated as basic units for engineering tissue constructs. As so, this review will focus on contributions made to each of these fields using spheroid models. EXPERT OPINION: Multi-cellular spheroids are rich in biological content and mimic better the in vivo environment than 2D cell culture. New technologies to form and analyze spheroids are rapidly increasing their adoption and expanding their applications.
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Cites
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“One such model, the differential adhesion hypothesis (DAH) posits that cell types self-segregate due to differences in cell-to-cell adhesion or apparent surface tension, with those cells of highest cohesion (like-to-like adhesion) sorting to the inside of a spheroid and those cells with lower cohesion sorting to the o…”
BackgroundMethodGene expression perturbation in vitro—A growing case for three-dimensional (3D) culture systems
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Cited by
Advances in multicellular spheroids formation
Cui, X., Hartanto, Y., & Zhang, H. (2017) · Journal of The Royal Society Interface · doi:10.1098/rsif.2016.0877 · Cited by 508
Spheroid Culture of Mesenchymal Stem Cells
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3D cell culture systems modeling tumor growth determinants in cancer target discovery
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Three-Dimensional Spheroids as In Vitro Preclinical Models for Cancer Research
Pinto, B., Henriques, A. C., Silva, P. M. A., & Bousbaa, H. (2020) · Pharmaceutics · doi:10.3390/pharmaceutics12121186 · Cited by 440
Life is 3D: Boosting Spheroid Function for Tissue Engineering
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Metadata
- Authors
- Achilli, T.-M., Meyer, J., & Morgan, J. R.
- Venue
- Expert Opinion on Biological Therapy
- Publisher
- Informa UK Limited
- Published
- 2012-07-12
- Volume
- 12
- Issue
- 10
- Pages
- 1347-1360
- ISSN
- 1471-2598 (ISSN-L)
- 1744-7682
- OpenAlex
- W2154555172
- Cited by
- 572
- References
- 13
- FWCI
- 8.89
- Type
- journal article
- Language
- en
- Open access
- green
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- Achilli, T.-M., Meyer, J., & Morgan, J. R. (2012). Advances in the formation, use and understanding of multi-cellular spheroids. Expert Opinion on Biological Therapy. https://doi.org/10.1517/14712598.2012.707181
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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