The DOI matches its Crossref record; 25 of the 37 references it cites were found in a registry or an index, and 12 are not checked yet; none of the 43 sources its claims cite is checked for support yet.
Aggrecan Directs Extracellular Matrix-Mediated Neuronal Plasticity
Open accessAbstract
In the adult brain, the extracellular matrix (ECM) influences recovery after injury, susceptibility to mental disorders, and is in general a strong regulator of neuronal plasticity. The proteoglycan aggrecan is a core component of the condensed ECM structures termed perineuronal nets (PNNs), and the specific role of PNNs on neural plasticity remains elusive. Here, we genetically targeted the Acan gene encoding for aggrecan using a novel animal model. This allowed for conditional and targeted loss of aggrecan in vivo, which ablated the PNN structure and caused a shift in the population of parvalbumin-expressing inhibitory interneurons toward a high plasticity state. Selective deletion of the Acan gene in the visual cortex of male adult mice reinstated juvenile ocular dominance plasticity, which was mechanistically identical to critical period plasticity. Brain-wide targeting improved object recognition memory. SIGNIFICANCE STATEMENT The study provides the first direct evidence of aggrecan as the main functional constituent and orchestrator of perineuronal nets (PNNs), and that loss of PNNs by aggrecan removal induces a permanent state of critical period-like plasticity. Loss of aggrecan ablates the PNN structure, resulting in invoked juvenile plasticity in the visual cortex and enhanced object recognition memory.
Checked 2026-10-06 · 12 references not checked yet
Overview
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Written from the paper’s full text.
TL;DR
Using a conditional Acan knockout mouse, this study shows that aggrecan is required for perineuronal net (PNN) assembly and that removing it in adult visual cortex reinstates juvenile-like ocular dominance plasticity and improves object recognition memory. It identifies aggrecan as a main functional constituent and orchestrator of PNNs.
Key findings
- Brain-wide conditional deletion of Acan (Acan-loxP +/+ CreNes) caused complete loss of WFA-labeled PNNs and prevented aggregation of other PNN components; heterozygous Acan-loxP +/-CreNes mice had anatomically normal PNNs but reduced WFA staining intensity (WFA ANOVA p=0.000244; Tukey heterozygous vs targeted p=0.0001934).
- Both heterozygous and homozygous Acan knockout mice showed a shift toward lower parvalbumin (PV) expression in cortical PV+ interneurons compared with WT and Acan-loxP +/+ controls (>150 PV+ cells per group; ANOVA p<2e-16), suggesting a high-plasticity network state.
- Local AAV9.Cre injection into adult V1 abolished aggrecan and WFA labeling by 8 weeks (WFA intensity lower in injected vs contralateral hemisphere, p=0.0006, paired t-test, n=6 mice). Four days of monocular deprivation produced a significant ocular dominance shift in AAV9.Cre-injected Acan-loxP +/+ mice (p=0.003, n=7), with increased ipsilateral responses (p=0.02) and reduced contralateral responses (p=0.009), while no effect was seen in non-injected Acan-loxP +/+ mice (p=0.21, n=4) or AAV9.Cre-injected Acan-loxP +/- mice (p=0.32, n=3).
- In spontaneous object recognition, Acan-loxP +/+ CreNes mice retained memory at 24 hours, whereas WT, Acan-loxP +/+, and Acan-loxP +/-CreNes groups showed significant decline from 3-hour to 24-hour delays (WT p=0.02547; homozygous Acan-loxP +/+ p=0.001154; heterozygous Acan-loxP +/-CreNes p=0.01658; Acan-loxP +/+ CreNes p=0.3768).
Methods
- A conditional Acan-loxP mouse was generated from EUCOMM tm1a embryonic stem cells, converted to the tm1c allele, and crossed with Nestin-CRE for brain-wide deletion; local adult deletion used AAV9.hSyn.HI.eGFP-Cre injection into primary visual cortex.
- Histology and biochemistry included WFA and aggrecan staining, immunohistochemistry for PNN components and PV, Western blotting, and qPCR; plasticity was assessed by optical imaging of intrinsic signals before and after 4-day monocular deprivation, and memory by spontaneous object recognition at 3-hour and 24-hour delays.
- Experiments used male mice: histology at 3–4 months (n=3 per group), ocular dominance testing at 5–7 months (n=4, 3, and 7 across groups), and object recognition at ~3 months (>7 per group). Statistical analysis used R or GraphPad Prism with Shapiro-Wilk/F tests, Welch’s or paired t-tests, and ANOVA with Tukey post hoc tests.
Limitations
- The paper does not include a dedicated limitations section; it states prior technical limitations such as off-target effects of chondroitinase ABC and embryonic lethality of Acan mutation, which motivated the new model.
- Evident from the text: all reported experiments used male mice only, ocular dominance groups were small (n=3–7), and memory was assessed only with spontaneous object recognition.
- Heterozygous Acan-loxP +/-CreNes mice showed reduced PNN intensity and altered PV expression but no detected behavioral alterations, indicating that some PNN/PV changes did not translate into the tested memory phenotype.
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30 Own result · 28 Not checked yet · 21 Own interpretation · 5 Own method
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Ablation of aggrecan was verified by immunohistochemistry and western blotting (Fig. 1c, d)”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > RESULTS
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In the paper
“although the intensity of the WFA staining was reduced in the heterozygote (Fig. 2b-e).”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > RESULTS
plaindirect- Asserted: passes — The authors put it forward as true.
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- Load-bearing: unclear — It is unclear whether the paper’s conclusions depend on it.
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- Rests on an assumption: The WFA histochemical signal specifically and quantitatively reflects the presence of PNNs.
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Targeting aggrecan in the brain of Acan-loxP +/+ CreNes mice was accompanied by complete loss of histochemical staining of PNNs in the cortex using the PNN marker Wisteria floribunda agglutinin (WFA) (Fig. 1b).”
- Also stated in: § Abstract · plain
“Loss of aggrecan ablates the PNN structure”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > RESULTS
plaindirect- Asserted: passes — The authors put it forward as true.
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- Load-bearing: passes — The paper’s conclusions depend on it.
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- Rests on an assumption: The WFA histochemical signal specifically and quantitatively reflects the presence of PNNs.
- Also stated in: § Abstract · plain
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“and reduced Acan mRNA in the brain (Fig. 1e).”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > RESULTS
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“Again, loss of aggrecan disrupted aggregation of the other PNN components (Fig. 3e)”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > RESULTS
plaindirect- Asserted: passes — The authors put it forward as true.
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- Rests on an assumption: The WFA histochemical signal specifically and quantitatively reflects the presence of PNNs.
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Our data also show that populations of PV+ interneurons in both homozygous and heterozygous Acan-loxP +/+ -CreNes mice are shifted to a state where they express less PV (Fig. 2).”
- Also stated in: § REFERENCES: · hedged
“Categorization of individual cells based on mean PV intensity indicated a shift towards a low PV network configuration in the Acan-loxP +/-Cre and Acan-loxP +/+ Cre groups compared to controls.”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > DISCUSSION
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In the paper
“Removal of aggrecan abolishes aggregation of PNNs, and essentially also incorporation of other ECM components into the structure (Fig 1 and 3).”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > DISCUSSION
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In the paper
“After 8 weeks all PNNs in the injected area in V1 were abolished.”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > REFERENCES:
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“alterations in their behavior were not detected.”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > RESULTS
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In the paper
“Categorization of individual cells based on mean PV intensity indicated a shift towards a low PV network configuration in the Acan-loxP +/-Cre and Acan-loxP +/+ Cre groups compared to controls.”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > REFERENCES:
hedgedcorrelational- Asserted: passes — The authors put it forward as true.
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- It may state two separate claims as one. 15
- Probably not a substantial claim. 14
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“In contrast, the non-targeted Acan-loxP +/+ mice with two intact alleles expressing Acan, and the targeted heterozygous Acan-loxP +/-CreNes mice having one intact allele, both had anatomically normal PNNs (Fig. 1b, f)”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > RESULTS
plain- Asserted: passes — The authors put it forward as true.
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It may state two separate claims as one.
- Rests on an assumption: The WFA histochemical signal specifically and quantitatively reflects the presence of PNNs.
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“The loss of aggrecan prevented aggregation of other PNN component ECM molecules into PNNs, effectively abolishing PNNs in the adult cortex (Fig. 1f, representative example from > 50 images per PNN component.Fig. 2a, overview of sampling).”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > RESULTS
plaindirect- Asserted: passes — The authors put it forward as true.
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It may state two separate claims as one.
- Rests on an assumption: The WFA histochemical signal specifically and quantitatively reflects the presence of PNNs.
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Although heterozygous Acan-loxP +/-CreNes have reduced PNNs intensity and altered PV expression”
- Also stated in: § REFERENCES: · plain
“Both Acan-loxP +/-Cre and Acan-loxP +/+ Cre groups displayed a significant reduction in average mean intensity per PV+ cell compared to WT and Acan-loxP +/+ controls.”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > RESULTS
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In the paper
“Instead, the shift in OD was driven both by a potentiation of ipsilateral responses and a reduction in contralateral responses”
- Also stated in: § REFERENCES: · plain
“The MD in AAV9.Cre injected Acan-loxP +/+ caused both increased responses to ipsilateral stimulation (p=0.02,paired t-test, n=7 mice) and reduced responses to contralateral stimulation (p=0.009,paired-test, n=7 mice)”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > RESULTS
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In the paper
“while no effect was observed in AAV9.Cre injected Acan-loxP +/-mice or non-injected Acan-loxP +/+ mice (Fig. 4c).”
- Also stated in: § REFERENCES: · plain
“Four days of MD did not affect ocular dominance in non-injected Acan-loxP +/+ mice (p=0.21,paired t-test, n=4 mice) or in heterozygous AAV9.Cre injected Acan-loxP +/-mice (p=0.32,paired t-test, n=3 mice).”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > RESULTS
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In the paper
“Closure of the CPs and concomitant loss of juvenile plasticity coincide with maturation of inhibitory circuits (Hensch, 2004) and condensation of extracellular matrix around subtypes of neurons.”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > INTRODUCTION
What the sources say
- Not checked yetcites Hensch, 2004
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In the paper
“However, the identity of the CSPG responsible for these effects has not been determined.”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > INTRODUCTION
What the sources say
- Not checked yetcites Pizzorusso et al., 2002
- Not checked yetcites Carulli et al., 2010
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In the paper
“Remarkably, enzymatically degrading the CS glycosaminoglycan (GAG) component (Pizzorusso et al., 2002) or ablating the cross-linking cartilage link protein 1 (Crtl1) (Carulli et al., 2010) restores juvenile plasticity in the adult animal and affects memory and learning, as revealed by behavioral tasks such as fear memory extinction (Gogolla et al., 2009) and spontaneous object recognition (Romberg”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > INTRODUCTION
What the sources say
- Not checked yetcites Pizzorusso et al., 2002
- Not checked yetcites Carulli et al., 2010
- Not checked yetcites Gogolla et al., 2009
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In the paper
“The proteoglycan aggrecan is a core component of the condensed ECM structures termed perineuronal nets (PNNs)”
- Also stated in: § INTRODUCTION · plain
“Here we investigate the role of the CSPG aggrecan, a major constituent of PNNs, encoded by Acan (Matthews et al., 2002).”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > Abstract
What the sources say
- Not checked yetcites Matthews et al., 2002
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- Also stated in: § INTRODUCTION · plain
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In the paper
“In the adult brain, the extracellular matrix (ECM) influences recovery after injury, susceptibility to mental disorders, and is in general a strong regulator of neuronal plasticity.”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > Abstract
What the sources say
- Not checked yetcites Pizzorusso et al., 2002
- Not checked yetcites Carulli et al., 2010
- Not checked yetcites Gogolla et al., 2009
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In the paper
“The increased plasticity after PNN removal could also be a direct consequence of neuronal activity changes in PV + cells.”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > DISCUSSION
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In the paper
“the specific role of PNNs on neural plasticity remains elusive.”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > Abstract
What the sources say
- Not checked yetcites Pizzorusso et al., 2002
- Not checked yetcites Carulli et al., 2010
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In the paper
“The maze and all objects were wiped down with 70% EtOH in between each testing session in order to avoid any olfactory bias that could have been introduced during the previous testing session.”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > Brain-wide and developmental Cre-lox recombination by Nestin-CRE
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In the paper
“Recently, parvalbumin (PV) expression on GABAergic interneurons has been shown to be influenced by PNNs in the hippocampus (Yamada et al., 2015)”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > RESULTS
What the sources say
- Not checked yetcites Yamada et al., 2015
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In the paper
“The three globular domains; G1, G2 and G3 interact with several matrix molecules aiding cross-linking, which could explain the failure of other components to aggregate in its absence.”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > DISCUSSION
hedgedconditional- Asserted: passes — The authors put it forward as true.
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- Rests on an assumption: Perineuronal net structure is based on tight cross-linking of matrix proteins, chondroitin sulfate and hyaluronic acid polysaccharides forming a condensed compact matrix, and on negatively charged sulfation patterns allowing specific interactions with positively charged domains in PNN interacting proteins.
In the paper
“Animals were tested after three and 24 hour delays, at which times ChABC-treated and Crtl1 knockout animals show enhanced memory retention (Romberg et al., 2013).”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > RESULTS
What the sources say
- Not checked yetcites Romberg et al., 2013
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In the paper
“The timeframe of this shift is faster than what has been observed in CP aged mice (Sato and Stryker, 2008), but is in line with previous work on adult mice with induced plasticity (Fu et al., 2015).”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > DISCUSSION
What the sources say
- Not checked yetcites Sato and Stryker, 2008
- Not checked yetcites Fu et al., 2015
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In the paper
“Several interventions affecting PNNs have effects on learning and memory (Gogolla et al., 2009;Carulli et al., 2010;Romberg et al., 2013).”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > RESULTS
What the sources say
- Not checked yetcites Gogolla et al., 2009
- Not checked yetcites Carulli et al., 2010
- Not checked yetcites Romberg et al., 2013
plaindirect- Asserted: passes — The authors put it forward as true.
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In the paper
“Others have suggested that the level of PV in PV+ interneurons determine if the network is in a state of high or low plasticity (Donato et al., 2013)”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > DISCUSSION
What the sources say
- Not checked yetcites Donato et al., 2013
hedgeddirect- Asserted: fails — The authors may not put it forward as true.
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In the paper
“The presentation of the object pairs and their appearance in either the left or right sample arm were counterbalanced so that a particular object in a set or a particular side might be novel for one animal and not for another to avoid unforeseen bias.”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > Brain-wide and developmental Cre-lox recombination by Nestin-CRE
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In the paper
“and the differentiation state and activity of PV+ interneurons relate to learning and memory formation (Donato et al., 2013).”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > RESULTS
What the sources say
- Not checked yetcites Donato et al., 2013
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In the paper
“To reduce variance in antibody binding all groups used for quantification were histologically stained in batches using the same conditions and antibody solutions.”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > Brain-wide and developmental Cre-lox recombination by Nestin-CRE
plaindirect- Asserted: passes — The authors put it forward as true.
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In the paper
“Indeed, the ratio of 4-and 6-sulfated CS influences neuronal plasticity (Miyata et al., 2012).”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > DISCUSSION
What the sources say
- Not checked yetcites Miyata et al., 2012
plaindirect- Asserted: passes — The authors put it forward as true.
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In the paper
“The tracer injections were intentionally targeted to a more medial part of V1 to prevent leakage into the neighboring V2L region.”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > Brain-wide and developmental Cre-lox recombination by Nestin-CRE
plaindirect- Asserted: passes — The authors put it forward as true.
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In the paper
“A shift in the distribution of WFA histochemical labeling intensity has been suggested to correlate with the degree of neuronal plasticity in learning and memory (Balmer et al., 2009).”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > RESULTS
What the sources say
- Not checked yetcites Balmer et al., 2009
hedgedcorrelational- Asserted: passes — The authors put it forward as true.
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Probably not a substantial claim. The paper’s conclusions may not depend on it.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Our data suggest that aggrecan plays a key role for the PNN structure, possibly through the large number of glycan chains that it carries, but clearly by affecting formation.”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > DISCUSSION
boosteddirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
It may state two separate claims as one.
- Rests on an assumption: Perineuronal net structure is based on tight cross-linking of matrix proteins, chondroitin sulfate and hyaluronic acid polysaccharides forming a condensed compact matrix, and on negatively charged sulfation patterns allowing specific interactions with positively charged domains in PNN interacting proteins.
None of the sources cited for this claim have been checked yet.
In the paper
“Longer lasting sensory deprivation has been shown to induce some OD plasticity also in adult mice, but the mechanism differs from juvenile plasticity (Sawtell et al., 2003;Ranson et al., 2012).”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > RESULTS
What the sources say
- Not checked yetcites Sawtell et al., 2003
- Not checked yetcites Ranson et al., 2012
plaindirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
It may state two separate claims as one.
None of the sources cited for this claim have been checked yet.
In the paper
“Recently, aggrecan and PNNs have been implied in various psychiatric disorders (Pantazopoulos et al., 2015), and in the progression of neurodegenerative diseases (Suttkus et al., 2016).”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > DISCUSSION
What the sources say
- Not checked yetcites Pantazopoulos et al., 2015
- Not checked yetcites Suttkus et al., 2016
hedgedcorrelational- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: fails — The paper’s conclusions may not depend on it.
- Based: passes — The paper gives a reason why it holds.
Probably not a substantial claim. The paper’s conclusions may not depend on it. It may state two separate claims as one.
The paper relies on this without support.
The WFA histochemical signal specifically and quantitatively reflects the presence of PNNs.
“using the PNN marker Wisteria floribunda agglutinin (WFA)”
“PNNs detected by WFA were absent in the brain.”
The paper relies on this without support.
The mouse visual cortex is a valid model for studying critical period plasticity.
“Selective deletion of the Acan gene in the visual cortex of male adult mice reinstated juvenile ocular dominance plasticity”
The paper relies on this without support.
The role of aggrecan, the backbone of perineuronal nets, in plasticity regulation remains to be investigated, and a full picture of plasticity regulation is lacking.
“Although several PNN CSPGs have been linked to plasticity regulation, the role of aggrecan, the backbone of PNN remains to be investigated, and a full picture of plasticity regulation is lacking.”
The paper relies on this without support.
Aggrecan-containing perineuronal nets play an essential role in regulation of brain plasticity.
“It has been suggested that aggrecan-containing PNNs play an essential role in regulation of brain plasticity.”
The paper relies on this without support.
Condensation of perineuronal nets in sensory cortices correlates with closure of periods of heightened neuronal plasticity in sensory systems.
“Condensation of PNNs in sensory cortices correlates with closure of periods of heightened neuronal plasticity in sensory systems.”
The paper relies on this without support.
Monocular deprivation in adults normally affects only responses to ipsilateral eye stimulation.
“While monocular deprivation in adults normally effects only responses to ipsilateral eye stimulation”
The paper relies on this without support.
Decreased parvalbumin expression and synapses are associated with hippocampal learning, while fear conditioning leads to a high-parvalbumin state.
“Decreased PV expression and synapses are associated with hippocampal learning, while fear conditioning leads to a high-PV state.”
The paper relies on this without support.
Parvalbumin-positive neuron-controlled shifts in network state are an integrated mechanism of learning and memory acquisition.
“PV+ neuron-controlled shifts in network state are suggested as an integrated mechanism of learning and memory acquisition.”
The paper relies on this without support.
ChABC treatment to degrade perineuronal nets shifted the network to a low-parvalbumin state, indicating that the glycans on CSPGs are a key component in controlling parvalbumin interneuron influence on network state.
“ChABC treatment to degrade PNNs shifted the network to a low-PV state, indicating that the glycans on CSPGs are a key component in controlling PV interneuron influence on network state.”
The paper relies on this without support.
Mounting evidence from other brain structures supports a broader role for perineuronal nets where they affect behavior, memory and learning processes.
“Mounting evidence from other brain structures supports a broader role for PNNs where they affect behavior, memory and learning processes.”
The paper relies on this without support.
Perineuronal net structure is based on tight cross-linking of matrix proteins, chondroitin sulfate and hyaluronic acid polysaccharides forming a condensed compact matrix, and on negatively charged sulfation patterns allowing specific interactions with positively charged domains in PNN interacting proteins.
“These mechanisms are based on the distinctive PNN structure; the tight cross-linking structures of matrix proteins, CS and hyaluronic acid polysaccharides forming a condensed compact matrix surrounding the cell soma and proximal dendrites, and the ability of the negatively charged sulfation patterns of the CS to allow specific interactions with positively charged domains in PNN interacting”
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“In line with this, digestion of CSPGs in the primary visual cortex in rats or deletion of aggrecan in mice decreases inhibitory activity, causing the network to revert to an immature juvenile state and an increased level of activity-dependent plasticity [47, 69].”
From The extracellular matrix and perineuronal nets in memory (2022) · cites this paper as [69]
Experimental — accuracy not yet measuredThe cited source says what the claim attributes to it.
In the citing paper
“Similarly, disaggregation of PNNs by genetic deletion of aggrecan shifts the population of PV inhibitory interneurons toward a juvenile-like plasticity state, accompanied by increased performance in the SOR memory task [69].”
From The extracellular matrix and perineuronal nets in memory (2022) · cites this paper as [69]
In the citing paper
“enhanced plasticity and learning is observed following experimental PNN ablation [23, 24]”
From Microglia facilitate loss of perineuronal nets in the Alzheimer's disease brain (2020) · cites this paper as [24]
In the citing paper
“Finally, disease-related effects on PNNs were validated at 12mo by staining for aggrecan, a core PNN CSPG [24], which confirmed the morphological abnormalities as well as the loss of 5xFAD subiculum and cortical PNNs at this timepoint (p<0.001, p<0.05; Fig. S2D-F).”
From Microglia facilitate loss of perineuronal nets in the Alzheimer's disease brain (2020) · cites this paper as [24]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
Each contradiction between two passages listed here was confirmed by an independent check.
Rated minor: it would not touch a result — a value no result rests on, a p-value that stays on the same side of the significance level, or a conflict that is clerical, resolved elsewhere in the paper, or outside its own findings.
In the paper
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > RESULTS
“In this paradigm, depicted in figure 5a, animals (> 7 per group) identify novel from familiar objects (choice phase) following a delay from familiar object presentation (sample phase).”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > EXPERIMENTAL DESIGN AND STATISTICAL ANALYSIS
“There were 7, 9 (which of one was singly housed), 10 and 7 male animals of ~3 months of age originating same line of breeding in each of the 4 experimental groups; WT, Acan-loxP (+/+) , Acan-loxP (+/-) Cre, and Acan-loxP (+/+) Cre.”
Rated minor: it would not touch a result — a value no result rests on, a p-value that stays on the same side of the significance level, or a conflict that is clerical, resolved elsewhere in the paper, or outside its own findings.
In the paper
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > RESULTS
“AAV9.hSyn.HI.eGFP.WPRE.SV40 (AAV9.Cre) carrying Cre recombinase”
§ Aggrecan directs extracellular matrix mediated neuronal plasticity > Brain-wide and developmental Cre-lox recombination by Nestin-CRE
“AAV9.hSyn.HI.eGFP-Cre.WPRE.SV40 (Catalog number AV-9-PV1848)”
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Metadata
- Authors
- Rowlands, D., Lensjø, K. K., Dinh, T., Yang, S., Andrews, M. R., Hafting, T., Fyhn, M., Fawcett, J. W., & Dick, G.
- Affiliations
- University of Oslo (NO)
- University of Cambridge (GB)
- University of Southampton (GB)
- Czech Academy of Sciences (CZ)
- ORCID
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2018-10-03
- Volume
- 38
- Issue
- 47
- Pages
- 10102-10113
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6596198
- OpenAlex
- W2894865807
- Semantic Scholar
- 9d76c8f2d73c04f3367a993907c15081487d05ea
- Cited by
- 204
- References
- 37
- Citations by year
- 2026: 20 · 2025: 40 · 2024: 30 · 2023: 23 · 2022: 33 · 2021: 17 · 2020: 26 · 2019: 14 · 2018: 1
- FWCI
- 5.33
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- ePrints Soton (University of Southampton) (submittedVersion)
- Funders
- Christopher and Dana Reeve Foundation
- Universitetet i Oslo
- Norges Forskningsråd (231248)
- Medical Research Council (MR/R004544/1, MR/R004463/1)
- Biotechnology and Biological Sciences Research Council (BB/N008189/1)
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
- Keywords
- aggrecan, perineuronal nets, extracellular matrix, neuroplasticity, parvalbumin interneurons, ocular dominance plasticity, critical period plasticity, visual cortex, object recognition memory, conditional gene deletion, proteoglycans
- MeSH terms
- Animals, Cell Line, Extracellular Matrix, Mice, Inbred C57BL, Mice, Transgenic, Nerve Net, Neuronal Plasticity, Photic Stimulation, Visual Cortex, Mice, Knockout, Mice, Aggrecans
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