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Visualization of Microtubule Growth in Cultured Neurons via the Use of EB3-GFP (End-Binding Protein 3-Green Fluorescent Protein)
Open accessAbstract
Several microtubule binding proteins, including CLIP-170 (cytoplasmic linker protein-170), CLIP-115, and EB1 (end-binding protein 1), have been shown to associate specifically with the ends of growing microtubules in non-neuronal cells, thereby regulating microtubule dynamics and the binding of microtubules to protein complexes, organelles, and membranes. When fused to GFP (green fluorescent protein), these proteins, which collectively are called +TIPs (plus end tracking proteins), also serve as powerful markers for visualizing microtubule growth events. Here we demonstrate that endogenous +TIPs are present at distal ends of microtubules in fixed neurons. Using EB3-GFP as a marker of microtubule growth in live cells, we subsequently analyze microtubule dynamics in neurons. Our results indicate that microtubules grow slower in neurons than in glia and COS-1 cells. The average speed and length of EB3-GFP movements are comparable in cell bodies, dendrites, axons, and growth cones. In the proximal region of differentiated dendrites ∼65% of EB3-GFP movements are directed toward the distal end, whereas 35% are directed toward the cell body. In more distal dendritic regions and in axons most EB3-GFP dots move toward the growth cone. This difference in directionality of EB3-GFP movements in dendrites and axons reflects the highly specific microtubule organization in neurons. Together, these results suggest that local microtubule polymerization contributes to the formation of the microtubule network in all neuronal compartments. We propose that similar mechanisms underlie the specific association of CLIPs and EB1-related proteins with the ends of growing microtubules in non-neuronal and neuronal cells.
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Microtubule dynamics in axons and dendrites
Baas, P. W., Slaughter, T., Brown, A., & Black, M. M. (1991) · Journal of Neuroscience Research · doi:10.1002/jnr.490300115 · Cited by 150
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Dompierre, J. P., Godin, J. D., Charrin, B. C., Cordelières, F. P., King, S. J., Humbert, S., & Saudou, F. (2007) · The Journal of Neuroscience · doi:10.1523/jneurosci.0037-07.2007 · Cited by 792
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Jaworski, J., Kapitein, L. C., Gouveia, S. M., Dortland, B. R., Wulf, P. S., Grigoriev, I., Camera, P., Spangler, S. A., Di Stefano, P., Demmers, J., et al. (2009) · Neuron · doi:10.1016/j.neuron.2008.11.013 · Cited by 643
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Witte, H., Neukirchen, D., & Bradke, F. (2008) · The Journal of Cell Biology · doi:10.1083/jcb.200707042 · Cited by 564
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Friedman, J. R., Webster, B. M., Mastronarde, D. N., Verhey, K. J., & Voeltz, G. K. (2010) · Journal of Cell Biology · doi:10.1083/jcb.200911024 · Cited by 393
Axonal transport: Driving synaptic function
Guedes-Dias, P., & Holzbaur, E. L. F. (2019) · Science · doi:10.1126/science.aaw9997 · Cited by 355
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Komarova, Y., De Groot, C. O., Grigoriev, I., Gouveia, S. M., Munteanu, E. L., Schober, J. M., Honnappa, S., Buey, R. M., Hoogenraad, C. C., Dogterom, M., et al. (2009) · Journal of Cell Biology · doi:10.1083/jcb.200807179 · Cited by 387
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Cai, D., McEwen, D. P., Martens, J. R., Meyhofer, E., & Verhey, K. J. (2009) · PLoS Biology · doi:10.1371/journal.pbio.1000216 · Cited by 300
van Beuningen, S. F. B., Will, L., Harterink, M., Chazeau, A., van Battum, E. Y., Frias, C. P., Franker, M. A. M., Katrukha, E. A., Stucchi, R., Vocking, K., et al. (2015) · Neuron · doi:10.1016/j.neuron.2015.11.012 · Cited by 225
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Dragestein, K. A., van Cappellen, W. A., van Haren, J., Tsibidis, G. D., Akhmanova, A., Knoch, T. A., Grosveld, F., & Galjart, N. (2008) · The Journal of Cell Biology · doi:10.1083/jcb.200707203 · Cited by 116
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Self-citationMcVicker, D. P., Awe, A. M., Richters, K. E., Wilson, R. L., Cowdrey, D. A., Hu, X., Chapman, E. R., & Dent, E. W. (2016) · Nature Communications · doi:10.1038/ncomms12741 · Cited by 74
A cryo-ET survey of microtubules and intracellular compartments in mammalian axons
Foster, H. E., Ventura Santos, C., & Carter, A. P. (2022) · Journal of Cell Biology · doi:10.1083/jcb.202103154 · Cited by 69
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Urbina, F. L., & Gupton, S. L. (2020) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2020.00133 · Cited by 48
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Nedozralova, H., Basnet, N., Ibiricu, I., Bodakuntla, S., Biertümpfel, C., & Mizuno, N. (2022) · Journal of Cell Biology · doi:10.1083/jcb.202106086 · Cited by 43
Ran-dependent TPX2 activation promotes acentrosomal microtubule nucleation in neurons
Chen, W.-S., Chen, Y.-J., Huang, Y.-A., Hsieh, B.-Y., Chiu, H.-C., Kao, P.-Y., Chao, C.-Y., & Hwang, E. (2017) · Scientific Reports · doi:10.1038/srep42297 · Cited by 41
Cellular cartography: Towards an atlas of the neuronal microtubule cytoskeleton
Iwanski, M. K., & Kapitein, L. C. (2023) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2023.1052245 · Cited by 38
He, L., van Beem, L., Snel, B., Hoogenraad, C. C., & Harterink, M. (2022) · PLOS Biology · doi:10.1371/journal.pbio.3001855 · Cited by 16
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Metadata
- Authors
- Stepanova, T., Slemmer, J., Hoogenraad, C. C., Lansbergen, G., Dortland, B., De Zeeuw, C. I., Grosveld, F., van Cappellen, G., Akhmanova, A., & Galjart, N.
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2003-04-01
- Volume
- 23
- Issue
- 7
- Pages
- 2655-2664
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- OpenAlex
- W2113144687
- Cited by
- 698
- References
- 1
- FWCI
- 5.14
- Type
- journal article
- Language
- en
- Open access
- bronze
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