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Cited by 137Neuroscience and Neuropharmacology ResearchNerve injury and regenerationNeurogenesis and neuroplasticity mechanisms

Brain-derived neurotrophic factor mediates activity-dependent dendritic growth in nonpyramidal neocortical interneurons in developing organotypic cultures

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Jin, Xiaoming, Hu, Hang, Mathers, Peter H., Agmon, Ariel·2003·The Journal of Neuroscience
DOI 10.1523/jneurosci.23-13-05662.2003PMC PMC6741232
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Abstract

Brain-derived neurotrophic factor (BDNF) promotes postnatal maturation of GABAergic inhibition in the cerebral and cerebellar cortices, and its expression and release are enhanced by neuronal activity, suggesting that it acts in a feedback manner to maintain a balance between excitation and inhibition during development. BDNF promotes differentiation of cerebellar, hippocampal, and neostriatal inhibitory neurons, but its effects on the dendritic development of neocortical inhibitory interneurons remain unknown. Here, we show that BDNF mediates depolarization-induced dendritic growth and branching in neocortical interneurons. To visualize inhibitory interneurons, we biolistically transfected organotypic cortical slice cultures from neonatal mice with green fluorescent protein (GFP) driven by the glutamic acid decarboxylase (GAD)67 promoter. Nearly all GAD67–GFP-expressing neurons were nonpyramidal, many contained GABA, and some expressed markers of neurochemically defined GABAergic subtypes, indicating that GAD67–GFP-expressing neurons were GABAergic. We traced dendritic trees from confocal images of the same GAD67–GFP-expressing neurons before and after a 5 d growth period, and quantified the change in total dendritic length (TDL) and total dendritic branch points (TDBPs) for each neuron. GAD67–GFP-expressing neurons growing in control medium exhibited a 20% increase in TDL, but in 200 ng/ml BDNF or 10 m m KCl, this increase nearly doubled and was accompanied by a significant increase in TDBPs. Blocking action potentials with TTX did not prevent the BDNF-induced growth, but antibodies against BDNF blocked the growth-promoting effect of KCl. We conclude that BDNF, released by neocortical pyramidal neurons in response to depolarization, enhances dendritic growth and branching in nearby inhibitory interneurons.

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  • BDNF is a neurotrophic factor that stimulates dendritic outgrowth and synaptogenesis.

    “MeCP2 knock-out animals have significantly perturbed synthesis and release of BDNF, a neurotrophic factor that stimulates dendritic outgrowth and synaptogenesis (Jin et al., 2003;Wang et al., 2006;Rex et al., 2007).”

    From The Environmental Neurotoxicant PCB 95 Promotes Synaptogenesis via Ryanodine Receptor-Dependent miR132 Upregulation (2014) · cites this paper as Jin et al., 2003

  • MeCP2 knock-out animals have significantly perturbed synthesis and release of BDNF.

    “MeCP2 knock-out animals have significantly perturbed synthesis and release of BDNF, a neurotrophic factor that stimulates dendritic outgrowth and synaptogenesis (Jin et al., 2003;Wang et al., 2006;Rex et al., 2007).”

    From The Environmental Neurotoxicant PCB 95 Promotes Synaptogenesis via Ryanodine Receptor-Dependent miR132 Upregulation (2014) · cites this paper as Jin et al., 2003

    The Results section reports that BDNF promotes dendritic growth in cultured neocortical interneurons, supporting the sentence’s description of BDNF. But the claim under review is specifically about perturbed BDNF synthesis and release in MeCP2 knock-out animals. No passage in our copy addresses MeCP2 knock-out animals or that perturbation (searched: MeCP2 knock-out, BDNF synthesis and release). (LLM, conf=0.76)

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1 of the 127 references couldn’t be matched to a known paper.

Research works printed but not linked: 1

  1. Mugnaini E, Oertel WH (1985) An atlas of the distribution of GABAergic neurons and terminals in the rat CNS as revealed by GAD immunohisto- chemistry. In: Handbook of chemical neuroanatomy, Vol 4 (Bjorklund A, Hokfelt T, eds), pp. 436 -608. Amsterdam: Elsevier.

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Authors
Jin, X., Hu, H., Mathers, P. H., & Agmon, A.
Affiliations
West Virginia University (US)
ORCID
Venue
The Journal of Neuroscience
Publisher
Society for Neuroscience
Published
2003-07-02
Volume
23
Issue
13
Pages
5662-5673
ISSN
  • 0270-6474 (ISSN-L)
  • 1529-2401
DOI
10.1523/jneurosci.23-13-05662.2003
PMC
PMC6741232
OpenAlex
W2150347274
Cited by
137
References
127
Citations by year
2026: 2 · 2025: 3 · 2023: 1 · 2022: 3 · 2021: 1 · 2020: 1 · 2019: 3 · 2018: 4 · 2017: 6 · 2016: 7 · 2015: 8 · 2014: 4 · 2013: 5 · 2012: 6
FWCI
4.17
Type
journal article
Language
en
Open access
Locations
  • Journal of Neuroscience (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r29hd033463-06)
  • National Eye Institute (R29EY012152, EY 12152, R01EY012152)
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD33463)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
Keywords
brain-derived neurotrophic factor, dendritic growth, dendritic branching, neocortical interneurons, GABAergic inhibition, glutamic acid decarboxylase 67, depolarization, potassium chloride depolarization, action potential, dendritic length
MeSH terms
Animals, Animals, Newborn, Cell Division, Cells, Cultured, Dendrites, gamma-Aminobutyric Acid, Glutamate Decarboxylase, Immunohistochemistry, Interneurons, Isoenzymes, Luminescent Proteins, Nerve Growth Factors, Neural Inhibition, Promoter Regions, Genetic, Time Factors, Brain-Derived Neurotrophic Factor, Biolistics, Neocortex, Green Fluorescent Proteins, Mice, In Vitro Techniques

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