Requirement for BDNF in activity-dependent survival of cortical neurons
Abstract
Cultured embryonic cortical neurons from rats were used to explore mechanisms of activity-dependent neuronal survival. Cell survival was increased by the activation of voltage-sensitive calcium channels (VSCCs) but not by activation of N-methyl-D-aspartate receptors. These effects correlated with the expression of brain-derived neurotrophic factor (BDNF) induced by these two classes of calcium channels. Antibodies to BDNF (which block intracellular signaling by BDNF, but not by nerve growth factor, NT3, or NT4/5) reduced the survival of cortical neurons and reversed the VSCC-mediated increase in survival. Thus, endogenous BDNF is a trophic factor for cortical neurons whose expression is VSCC-regulated and that functions in the VSCC-dependent survival of these neurons.
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BDNF has been linked to the mechanism of action of antidepressants.
“In this context, BDNF has been linked to the mechanism of action of antidepressants (Duman et al., 1997; Ghosh et al., 1994; Monteggia et al., 2004; Duman and Monteggia, 2006).”
From BDNF - a key transducer of antidepressant effects (2016) · cites this paper as Ghosh et al., 1994
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- Authors
- Ghosh, A., Carnahan, J., & Greenberg, M. E.
- Affiliations
- Harvard University (US)
- Amgen (United States) (US)
- Venue
- Science
- Published
- 1994-03-18
- Volume
- 263
- Issue
- 5153
- Pages
- 1618-1623
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2069527370
- Cited by
- 989
- References
- 2
- Citations by year
- 2026: 10 · 2025: 15 · 2024: 16 · 2023: 24 · 2022: 24 · 2021: 22 · 2020: 15 · 2019: 23 · 2018: 25 · 2017: 29 · 2016: 28 · 2015: 26 · 2014: 18 · 2013: 25 · 2012: 24
- FWCI
- 19.42
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Neurological Disorders and Stroke (NS 28829)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Keywords
- brain-derived neurotrophic factor, cortical neurons, voltage-gated calcium channels, NMDA receptor, trophic factors, embryonic neurons, calcium signaling
- MeSH terms
- Animals, Antibodies, Cell Division, Cell Survival, Cells, Cultured, Cerebral Cortex, Embryo, Mammalian, Glutamates, Nerve Growth Factors, Nerve Tissue Proteins, Neurons, Phosphorylation, Potassium Chloride, Calcium Channels, Signal Transduction, Receptors, N-Methyl-D-Aspartate, N-Methylaspartate, Cyclic AMP Response Element-Binding Protein, Glutamic Acid, Brain-Derived Neurotrophic Factor, Rats
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