CoChat
Sign Up
Cited by 143Sleep and Wakefulness ResearchAdenosine and Purinergic SignalingNeurotransmitter Receptor Influence on Behavior

The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.

Adenosine A 1 and A 2A Receptors in Mouse Prefrontal Cortex Modulate Acetylcholine Release and Behavioral Arousal

Open access
Van Dort, Christa J., Baghdoyan, Helen A., Lydic, Ralph·2009·The Journal of Neuroscience
DOI 10.1523/jneurosci.4111-08.2009PMC PMC3430130
Open this paper in CoChat — free accountOpen PDF

Abstract

During prolonged intervals of wakefulness, brain adenosine levels rise within the basal forebrain and cortex. The view that adenosine promotes sleep is supported by the corollary that N -methylated xanthines such as caffeine increase brain and behavioral arousal by blocking adenosine receptors. The four subtypes of adenosine receptors are distributed heterogeneously throughout the brain, yet the neurotransmitter systems and brain regions through which adenosine receptor blockade causes arousal are incompletely understood. This study tested the hypothesis that adenosine A 1 and A 2A receptors in the prefrontal cortex contribute to the regulation of behavioral and cortical arousal. Dependent measures included acetylcholine (ACh) release in the prefrontal cortex, cortical electroencephalographic (EEG) power, and time to waking after anesthesia. Sleep and wakefulness were also quantified after microinjecting an adenosine A 1 receptor antagonist into the prefrontal cortex. The results showed that adenosine A 1 and A 2A receptors in the prefrontal cortex modulate cortical ACh release, behavioral arousal, EEG delta power, and sleep. Additional dual microdialysis studies revealed that ACh release in the pontine reticular formation is significantly altered by dialysis delivery of adenosine receptor agonists and antagonists to the prefrontal cortex. These data, and early brain transection studies demonstrating that the forebrain is not needed for sleep cycle generation, suggest that the prefrontal cortex modulates EEG and behavioral arousal via descending input to the pontine brainstem. The results provide novel evidence that adenosine A 1 receptors within the prefrontal cortex comprise part of a descending system that inhibits wakefulness.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1523/jneurosci.4111-08.2009

Cites

4 of 4 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Direct wakefulness consolidation indirectly enhances subsequent SWS, and may thus facilitate the hypothesized mechanism for trazodone's benefits.

    “In turn, direct wakefulness consolidation indirectly enhances subsequent SWS, and may thus facilitate the hypothesized mechanism for trazodone's benefits [27-29, 36]”

    From Long-Term Trazodone Use and Cognition: A Potential Therapeutic Role for Slow-Wave Sleep Enhancers (2019) · cites this paper as [36]

    The Results report that an adenosine A1 antagonist increased wakefulness and decreased NREM sleep in mice. The Discussion also reports increased slow-wave activity during NREM sleep in a sleep-deprivation context. Neither passage shows that consolidating wakefulness enhances subsequent slow-wave sleep or addresses trazodone’s benefits; no page numbers are available in our copy. (LLM, conf=0.73)

    Experimental — accuracy not yet measured

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 57

  1. 10.1016/S0893-133X(02)00290-7 · DOI
  2. Alaraj, 1998, Effects of caffeine on NMDA-evoked 45Ca2+ release in the rat dentate gyrus in vivo, Acta Neurobiol Exp (Wars), 58, 239, 10.55782/ane-1998-1278 · DOI
  3. 10.1016/0006-8993(84)90351-2 · DOI · Paper page
  4. 10.1097/00001756-200106130-00013 · DOI
  5. 10.1016/j.pneurobio.2004.06.004 · DOI
  6. Basheer, 2007, Sleep deprivation upregulates A1 adenosine receptors in the rat basal forebrain, Neuroreport, 18, 1895, 10.1097/WNR.0b013e3282f262f6 · DOI
  7. 10.1016/0006-8993(95)00590-M · DOI
  8. 10.1016/0014-4886(74)90079-X · DOI
  9. Carter, 1997, Hippocampal noradrenaline release in awake, freely moving rats is regulated by alpha-2 adrenoceptors but not by adenosine receptors, J Pharmacol Exp Ther, 281, 648, 10.1016/S0022-3565(24)36669-8 · DOI
  10. 10.1523/JNEUROSCI.0733-08.2008 · DOI
  11. 10.1111/j.1471-4159.2006.03700.x · DOI
  12. Crawley J (2000) Behavioral phenotyping of transgenic and knockout mice, What's wrong with my mouse? (Wiley-Liss, New York). · DOI
  13. 10.1021/jm00157a035 · DOI
  14. 10.1016/j.neuroscience.2003.08.045 · DOI
  15. Douglas, 2002, Prefrontal cortex acetylcholine release, EEG slow waves, and spindles are modulated by M2 autoreceptors in C57BL/6J mouse, J Neurophysiol, 87, 2817, 10.1152/jn.2002.87.6.2817 · DOI
  16. 10.1523/JNEUROSCI.5066-06.2007 · DOI
  17. 10.1016/S0896-6273(01)00285-9 · DOI
  18. 10.1016/S0306-4522(03)00031-9 · DOI
  19. 10.1016/j.brainresbull.2007.09.008 · DOI
  20. 10.1007/s00210-004-0970-1 · DOI
  21. 10.1117/1.1755233 · DOI
  22. 10.1016/0306-4522(96)00021-8 · DOI
  23. Jouvet, 1962, Research on the neural structures and responsible mechanisms in different phases of physiological sleep, Arch Ital Biol, 100, 125
  24. 10.1007/s002100000315 · DOI
  25. 10.1093/cercor/bhi079 · DOI · Paper page
  26. 10.1007/s002130051125 · DOI
  27. Leonard, 1997, Pontine nitric oxide modulates acetylcholine release, rapid eye movement sleep generation, and respiratory rate, J Neurosci, 17, 774, 10.1523/JNEUROSCI.17-02-00774.1997 · DOI
  28. Linden, 1999, Characterization of human A2B adenosine receptors: radioligand binding, western blotting, and coupling to G(q) in human embryonic kidney 293 cells and HMC-1 mast cells, Mol Pharmacol, 56, 705, 10.1016/S0026-895X(24)12531-X · DOI
  29. 10.1038/nature00817 · DOI
  30. 10.1016/S0014-2999(99)00659-7 · DOI
  31. 10.1038/sj.bjp.0705692 · DOI
  32. Lydic, 1993, Pedunculopontine stimulation alters respiration and increases ACh release in the pontine reticular formation, Am J Physiol, 264, R544
  33. 10.1016/S0306-4522(02)00561-4 · DOI
  34. 10.1046/j.0953-816x.2001.01619.x · DOI
  35. 10.1016/S0306-4522(00)00075-0 · DOI
  36. 10.1016/0013-4694(49)90066-8 · DOI
  37. 10.1016/0304-3940(96)12780-4 · DOI
  38. 10.1016/S0014-2999(96)00938-7 · DOI
  39. 10.1016/S0031-9384(97)00390-9 · DOI
  40. 10.1126/science.276.5316.1265 · DOI · Paper page
  41. 10.1016/S0306-4522(00)00220-7 · DOI
  42. 10.1053/smrv.2001.0201 · DOI
  43. 10.1179/016164105X21814 · DOI
  44. 10.1016/j.neuroscience.2007.06.057 · DOI
  45. Robert, 1998, Knockout mice: advantages and limitations for biological modeling. Definitions, principles and examples, Ann Dermatol Venereol, 125, 946
  46. Sonner, 1999, Mouse strain modestly influences minimum alveolar anesthetic concentration and convulsivity of inhaled compounds, Anesth Analg, 89, 1030, 10.1213/00000539-199910000-00039 · DOI · Paper page
  47. Stenberg, 2003, Sleep and its homeostatic regulation in mice lacking the adenosine A1 receptor, J Sleep Res, 12, 283, 10.1046/j.0962-1105.2003.00367.x · DOI
  48. Steriade, 1990, Neuronal activities in brain-stem cholinergic nuclei related to tonic activation processes in thalamocortical systems, J Neurosci, 10, 2541, 10.1523/JNEUROSCI.10-08-02541.1990 · DOI
  49. 10.1016/S0166-4328(00)00258-8 · DOI
  50. 10.1097/00000542-200304000-00018 · DOI
  51. 10.1097/00000542-200210000-00024 · DOI
  52. 10.1097/00000542-200506000-00015 · DOI
  53. 10.1159/000067831 · DOI
  54. Van Dongen, 2001, Caffeine eliminates psychomotor vigilance deficits from sleep inertia, Sleep, 24, 813, 10.1093/sleep/24.7.813 · DOI
  55. 10.1097/ALN.0b013e31818e3b1b · DOI
  56. Watson, 2006, In vivo measurements of neurotransmitters by microdialysis sampling, Anal Chem, 78, 1391, 10.1021/ac0693722 · DOI
  57. 10.1016/0006-8993(87)90046-1 · DOI

Cited by

Sorted by citations

Metadata

Authors
Van Dort, C. J., Baghdoyan, H. A., & Lydic, R.
Affiliations
  • Pediatrics and Genetics (US)
  • University of Michigan (US)
ORCID
Venue
The Journal of Neuroscience
Publisher
Society for Neuroscience
Published
2009-01-21
Volume
29
Issue
3
Pages
871-881
ISSN
  • 0270-6474 (ISSN-L)
  • 1529-2401
DOI
10.1523/jneurosci.4111-08.2009
PMC
PMC3430130
OpenAlex
W2088462720
Cited by
143
References
4
Citations by year
2026: 1 · 2025: 2 · 2024: 9 · 2023: 5 · 2022: 11 · 2021: 7 · 2020: 7 · 2019: 5 · 2018: 6 · 2017: 5 · 2016: 6 · 2015: 4 · 2014: 10 · 2013: 6 · 2012: 16
FWCI
8.14
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (MH45361)
  • National Heart, Lung, and Blood Institute (R01HL057120, R01HL040881, R01HL065272)
  • National Institute of Mental Health (R37MH045361, R01MH045361, MH-45361)
Topics
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Sleep and Wakefulness Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Adenosine and Purinergic Signaling
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
Keywords
adenosine A1 receptor, adenosine A2A receptor, prefrontal cortex, acetylcholine release, behavioral arousal, sleep regulation, wakefulness, EEG delta power, pontine reticular formation, adenosine receptor antagonists, caffeine
MeSH terms
Acetylcholine, Adenosine, Analysis of Variance, Animals, Arousal, Behavior, Animal, Caffeine, Chromatography, High Pressure Liquid, Electroencephalography, Electromyography, Male, Phenethylamines, Phosphodiesterase Inhibitors, Spectrum Analysis, Triazines, Triazoles, Xanthines, Prefrontal Cortex, Microdialysis, Receptor, Adenosine A1, Receptors, Adenosine A2, Mice, Adenosine A1 Receptor Antagonists, Adenosine A2 Receptor Antagonists

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review