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Methyl-CpG-Binding Domain Proteins: Readers of the Epigenome
Open accessAbstract
How DNA methylation is interpreted and influences genome regulation remains largely unknown. Proteins of the methyl-CpG-binding domain (MBD) family are primary candidates for the readout of DNA methylation as they recruit chromatin remodelers, histone deacetylases and methylases to methylated DNA associated with gene repression. MBD protein binding requires both functional MBD domains and methyl-CpGs; however, some MBD proteins also bind unmethylated DNA and active regulatory regions via alternative regulatory domains or interaction with the nucleosome remodeling deacetylase (NuRD/Mi-2) complex members. Mutations within MBD domains occur in many diseases, including neurological disorders and cancers, leading to loss of MBD binding specificity to methylated sites and gene deregulation. Here, we summarize the current state of knowledge about MBD proteins and their role as readers of the epigenome.
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“5mC readers including proteins containing a methyl-CpG-binding domain such as MECP2 or MBD proteins can recognize 5mC and recruit co-regulators to methylated DNA (25).”
From Dynamic DNA methylation: In the right place at the right time (2018) · cites this paper as [25]
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Metadata
- Authors
- Du, Q., Luu, P.-L., Stirzaker, C., & Clark, S. J.
- Affiliations
- Garvan Institute of Medical Research (AU)
- Venue
- Epigenomics
- Publisher
- Informa UK Limited
- Published
- 2015-04-30
- Volume
- 7
- Issue
- 6
- Pages
- 1051-1073
- ISSN
- 1750-1911 (ISSN-L)
- 1750-192X
- OpenAlex
- W2040875973
- Cited by
- 544
- References
- 191
- Citations by year
- 2026: 34 · 2025: 61 · 2024: 55 · 2023: 53 · 2022: 74 · 2021: 72 · 2020: 43 · 2019: 47 · 2018: 34 · 2017: 35 · 2016: 23 · 2015: 5
- FWCI
- 3.13
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Epigenomics (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Funders
- NHMRC Fellowship and NHMRC (1088144)
- Stirzaker and P-L Luu by NHMRC (1088144)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cancer-related gene regulation
- Keywords
- methyl-CpG binding domain proteins, DNA methylation, epigenetic reader protein, chromatin remodeling, histone deacetylase, gene repression, neurological disorders, cancer, gene expression regulation
- MeSH terms
- Animals, Binding Sites, Cell Differentiation, DNA-Binding Proteins, Gene Expression Regulation, Multigene Family, Humans, Mutation, Neoplasms, Protein Binding, Down-Regulation, CpG Islands, DNA Methylation, Genetic Predisposition to Disease, Gene Silencing, Epigenesis, Genetic, Protein Interaction Domains and Motifs, Cellular Reprogramming
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