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Cited by 715

The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.

Regulatory Mechanisms for Adipose Tissue M1 and M2 Macrophages in Diet-Induced Obese Mice

Open access
Fujisaka, Shiho, Usui, Isao, Bukhari, Agussalim, Ikutani, Masashi, Oya, Takeshi, Kanatani, Yukiko, Tsuneyama, Koichi, Nagai, Yoshinori, Takatsu, Kiyoshi, Urakaze, Masaharu, Kobayashi, Masashi, Tobe, Kazuyuki·2009·Diabetes
DOI 10.2337/db08-1475
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Abstract

OBJECTIVE: To characterize the phenotypic changes of adipose tissue macrophages (ATMs) under different conditions of insulin sensitivity. RESEARCH DESIGN AND METHODS: The number and the expressions of marker genes for M1 and M2 macrophages from mouse epididymal fat tissue were analyzed using flow cytometry after the mice had been subjected to a high-fat diet (HFD) and pioglitazone treatment. RESULTS: Most of the CD11c-positive M1 macrophages and the CD206-positive M2 macrophages in the epididymal fat tissue were clearly separated using flow cytometry. The M1 and M2 macrophages exhibited completely different gene expression patterns. Not only the numbers of M1 ATMs and the expression of M1 marker genes, such as tumor necrosis factor-α and monocyte chemoattractant protein-1, but also the M1-to-M2 ratio were increased by an HFD and decreased by subsequent pioglitazone treatment, suggesting the correlation with whole-body insulin sensitivity. We also found that the increased number of M2 ATMs after an HFD was associated with the upregulated expression of interleukin (IL)-10, an anti-inflammatory Th2 cytokine, in the adipocyte fraction as well as in adipose tissue. The systemic overexpression of IL-10 by an adenovirus vector increased the expression of M2 markers in adipose tissue. CONCLUSIONS: M1 and M2 ATMs constitute different subsets of macrophages. Insulin resistance is associated with both the number of M1 macrophages and the M1-to-M2 ratio. The increased expression of IL-10 after an HFD might be involved in the increased recruitment of M2 macrophages.

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Metadata

Authors
Fujisaka, S., Usui, I., Bukhari, A., Ikutani, M., Oya, T., Kanatani, Y., Tsuneyama, K., Nagai, Y., Takatsu, K., Urakaze, M., Kobayashi, M., & Tobe, K.
Venue
Diabetes
Publisher
American Diabetes Association
Published
2009-08-18
Volume
58
Issue
11
Pages
2574-2582
ISSN
  • 0012-1797 (ISSN-L)
  • 1939-327X
DOI
10.2337/db08-1475
OpenAlex
W2106466636
Cited by
715
References
18
FWCI
17.07
Type
journal article
Language
en
Open access
hybrid

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