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Confocal Laser Scanning Microscopy for Analysis of Pseudomonas aeruginosa Biofilm Architecture and Matrix Localization
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“It provides three-dimensional information on volume, live and dead ratios, height, surface coverage, and roughness.”
From Understanding Pseudomonas aeruginosa Biofilms: Quorum Sensing, c-di-GMP Signaling, and Emerging Antibiofilm Approaches (2026) · cites this paper as [237]
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“Confocal laser scanning microscopy (CLSM) remains a central method for tracking P. aeruginosa biofilm development, stress responses, and regression.”
From Understanding Pseudomonas aeruginosa Biofilms: Quorum Sensing, c-di-GMP Signaling, and Emerging Antibiofilm Approaches (2026) · cites this paper as [237]
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“Harmonized workflows reduce operator bias, which is critical when evaluating QS inhibitors or c-di-GMP ligands [237,238].”
From Understanding Pseudomonas aeruginosa Biofilms: Quorum Sensing, c-di-GMP Signaling, and Emerging Antibiofilm Approaches (2026) · cites this paper as [237]
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Citations
Cites
Biofilms: Survival Mechanisms of Clinically Relevant Microorganisms
Donlan, R. M., & Costerton, J. W. (2002) · Clinical Microbiology Reviews · doi:10.1128/cmr.15.2.167-193.2002 · Cited by 6,644
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Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development
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Extracellular DNA Required for Bacterial Biofilm Formation
Whitchurch, C. B., Tolker-Nielsen, T., Ragas, P. C., & Mattick, J. S. (2002) · Science · doi:10.1126/science.295.5559.1487 · Cited by 2,203
Novel Entries in a Fungal Biofilm Matrix Encyclopedia
Zarnowski, R., Westler, W. M., Lacmbouh, G. A., Marita, J. M., Bothe, J. R., Bernhardt, J., Lounes-Hadj Sahraoui, A., Fontaine, J., Sanchez, H., Hatfield, R. D., et al. (2014) · mBio · doi:10.1128/mbio.01333-14 · Cited by 315
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Cited by
Rumbaugh, K. P., & Sauer, K. (2020) · Nature Reviews Microbiology · doi:10.1038/s41579-020-0385-0 · Cited by 907
Mountcastle, S. E., Vyas, N., Villapun, V. M., Cox, S. C., Jabbari, S., Sammons, R. L., Shelton, R. M., Walmsley, A. D., & Kuehne, S. A. (2021) · npj Biofilms and Microbiomes · doi:10.1038/s41522-021-00214-7 · Cited by 130
Kiousi, D. E., Efstathiou, C., Tzampazlis, V., Plessas, S., Panopoulou, M., Koffa, M., & Galanis, A. (2023) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2023.1127256 · Cited by 41
Elbehiry, A., Marzouk, E., Edrees, H. M., Ibrahem, M., Alzahrani, S., Anagreyyah, S., Abualola, H., Alghamdi, A., Alzahrani, A., Jaber, M., et al. (2026) · Microorganisms · doi:10.3390/microorganisms14010109 · Cited by 34
Metadata
- Authors
- Reichhardt, C., & Parsek, M. R.
- Venue
- Frontiers in Microbiology
- Publisher
- Frontiers Media SA
- Published
- 2019-04-02
- Volume
- 10
- ISSN
- 1664-302X
- Cited by
- 145
- References
- 6
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