The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Phenolic Compounds and Antimicrobial Activity of Olive (Olea europaea L. Cv. Cobrançosa) Leaves
Open accessAbstract
We report the determination of phenolic compounds in olive leaves by reversed-phase HPLC/DAD, and the evaluation of their in vitro activity against several microorganisms that may be causal agents of human intestinal and respiratory tract infections, namely gram positive (Bacillus cereus, B. subtilis and Staphylococcus aureus), gram negative bacteria (Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae) and fungi (Candida albicans and Cryptococcus neoformans). Seven phenolic compounds were identified and quantified: caffeic acid, verbascoside, oleuropein, luteolin 7-O-glucoside, rutin, apigenin 7-O-glucoside and luteolin 4'-O-glucoside. At low concentrations olive leaves extracts showed an unusual combined antibacterial and antifungal action, which suggest their great potential as nutraceuticals, particularly as a source of phenolic compounds.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The cited source says what the claim attributes to it.
In the citing paper
“Additionally, in general, in the mixed form, individual components can modify their properties and interact synergistically, producing improved effects [18,25,39].”
From Comparison of Techniques and Solvents on the Antimicrobial and Antioxidant Potential of Extracts from Acacia dealbata and Olea europaea (2020) · cites this paper as [25]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
The crisis in antibiotic resistance
Neu, H. C. (1992) · Science · doi:10.1126/science.257.5073.1064 · Cited by 2,834 · cited 1×
“Olive leaves may be useful in cases where prolonged use of antibiotics encourage development of opportunistic infections [26], being especially effective against Klebsiella and Pseudomonas, two bacterial genera which pose a major resistance problem [27].”
BackgroundLiu, R. H. (2003) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/78.3.517s · Cited by 2,213
Antimicrobial effects of Finnish plant extracts containing flavonoids and other phenolic compounds
Rauha, J., Remes, S., Heinonen, M., Hopia, A., Kähkönen, M., Kujala, T. S., Pihlaja, K., Vuorela, H. J., & Vuorela, P. M. (2000) · International Journal of Food Microbiology · doi:10.1016/s0168-1605(00)00218-x · Cited by 1,143
Antimicrobial properties of phenolic compounds from berries
Puupponen‐Pimiä, R., Nohynek, L. J., Meier, C., Kähkönen, M., Heinonen, M., Hopia, A., & Oksman‐Caldentey, K. (2001) · Journal of Applied Microbiology · doi:10.1046/j.1365-2672.2001.01271.x · Cited by 1,055
Antioxidant activity of phenolics extracted from Olea europaea L. leaves
Benavente-Garcı́a, O., Castillo, J., Lorente, J., Ortuño, A., & Del Rio, J. A. (2000) · Food Chemistry · doi:10.1016/s0308-8146(99)00221-6 · Cited by 867
On the In-vitro Antimicrobial Activity of Oleuropein and Hydroxytyrosol
Bisignano, G., Tomaino, A., Cascio, R. L., Crisafi, G., Uccella, N., & Saija, A. (1999) · Journal of Pharmacy and Pharmacology · doi:10.1211/0022357991773258 · Cited by 532
Proestos, C., Chorianopoulos, N., Nychas, G. E., & Komaitis, M. (2005) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf040083t · Cited by 447
Mushrooms, Tumors, and Immunity: An Update
Borchers, A. T., Keen, C. L., & Gershwin, M. E. (2004) · Experimental Biology and Medicine · doi:10.1177/153537020422900507 · Cited by 381
Zhu, X., Zhang, H., & Lo, R. (2004) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf0490192 · Cited by 376
Antioxidant Activity of Hydroxytyrosol Acetate Compared with That of Other Olive Oil Polyphenols
Gordon, M. H., Paiva‐Martins, F., & de Almeida, M. P. (2001) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf000537w · Cited by 322
Research works printed but not linked: 2
- Samuelsson, 1951, The blood pressure lowering factor in leaves of Olea europaea, Farmacevtisk Revy, 15, 229
- Castillo, 2000, Antioxidant activity of phenolics from Olea europaea L. leaves, Food Chem., 68, 457, 10.1016/S0308-8146(99)00221-6 · DOI · Paper page
Cited by
Sorted by citations
Items 501–519 of 519
Extraction Technologies for Bioactive Compounds
Isa, K. M., & Saad, S. A. (2025) · Springer eBooks · doi:10.1007/978-981-96-6279-1_3
Thermal power plant proximity alters Olive composition and induces cytotoxicity in human cells
Gürbüz, E., AKSOY, E., Sandallı, A., Tetikoğlu, F. B., Şeker, E., Kıran, N. N., Tetikoğlu, S., Muratoğlu, H., Kaya, M. F., Turumtay, E. A., et al. (2025) · Scientific Reports · doi:10.1038/s41598-025-18066-y
“…with Silva et al. (2010), who identified nüzhenide and nüzhenide 11-methyl oleoside as the main secoiridoids in olive seeds using LC-DAD-MS, and with Pereira et al. (2007) 46 (cited in 1 , who reported oleuropein and these flavonoid glucosides as dominant phenolics in olive leaf extracts.”
Tartor, Y. H., El-Neshwy, W. M., Merwad, A. M. A., Abo El-Maati, M. F., Mohamed, R. E., Dahshan, H., & Mahmoud, H. I. (2020) · Figshare · doi:10.6084/m9.figshare.13191919
Tartor, Y. H., El-Neshwy, W. M., Merwad, A. M. A., AboElMaati, M. F., Mohamed, R. E., Dahshan, H., & Mahmoud, H. I. (2020) · Figshare · doi:10.6084/m9.figshare.13191922
Covaliov, E., Tatiana, C., Ruseva, O., Natalia, S., Olga, D., Vladislav, R., Chirsanova, A., & Nеtrеbа, N. (2025) · Food and Nutrition Sciences · doi:10.4236/fns.2025.1610086
Green Extraction of Polyphenols from Olive Flesh, Pits and Leaves by Supercritical CO₂
Burgaz, O., Yıldırım, İ., Sana, F., Baycan, A., Nadeem, H. Ş., & Polat, İ. (2025) · Current Perspectives on Medicinal and Aromatic Plants (CUPMAP) · doi:10.38093/cupmap.1754246
Rufchaei, R., Hoseinifar, S. H., Anzabi, M. P., Safari, R., Bourani, M. S., Ahmadnezhad, M., & Aramli, M. S. (2026) · Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology · doi:10.1016/j.cbpb.2026.111221
Al-Qarraawi, R. A. H., Al-Rahimy, S. K., & Al-Essa, R. A. (2026) · AIP conference proceedings · doi:10.1063/5.0322004
Saitta, A., Lopriore, M., Marino, M., Barp, L., Conchione, C., Moret, S., Plazzotta, S., Bastante, C. C., & Manzocco, L. (2026) · LWT · doi:10.1016/j.lwt.2026.119306
Effect of different crude extracts of Olive leaves on the growth of some pathogenic dermal fungi
Saleh, K. W., Yassin, I. A. M., & Muhammed, G. A. (2026) · AIP conference proceedings · doi:10.1063/5.0318559
Mohammadi, E., Norouzian, M. A., Assadi-Alamouti, A., Varzaneh, M. B., & Ranjbaran, M. (2026) · Veterinary and Animal Science · doi:10.1016/j.vas.2026.100721
“In vitro and in vivo studies have demonstrated that clove extract inhibits the growth of common enteric pathogens, scavenges reactive oxygen species, and suppresses inflammatory signaling pathways such as NF-κ B and MAPK (Pereira et al., 2007; S ´ anchez-Quesada et al., 2013; Hu et al., 2018).”
Avila, L. B., Pedroso, P. D. C., Moraes, C. C., Dotto, G. L., & da Rosa, G. S. (2026) · ACS Omega · doi:10.1021/acsomega.5c11399
Soltani, S., Hajji, S., Allouche, N., & Boufi, S. (2026) · Chemistry Africa · doi:10.1007/s42250-026-01746-1
“These compounds exhibit strong antimicrobial activity by disrupting microbial cell membranes, interfering with enzymatic processes, and scavenging free radicals, thereby effectively inhibiting the growth of spoilage-causing bacteria and fungi [ 40 , 47 – 49 ].”
Simões, L. A., Fernandes, N., Sousa, D., da Silva, B., Souza, A., & Dias, D. R. (2026) · AIMS Agriculture and Food · doi:10.3934/agrfood.2026023
Ediz, E. F., Bostancı, V. M., & Kavak, E. (2026) · Afyon Kocatepe University Journal of Sciences and Engineering · doi:10.35414/akufemubid.1875826
Guillamón, P. C., Domínguez-Lacueva, P., Cantalejo, M. J., Sánchez-Zamorano, L., & Arminaite, E. (2026) · Applied Food Research · doi:10.1016/j.afres.2026.102647
Palani, G., Anuradha, N., Rajagopal, P., Murali, R., Palanisamy, C. P., Veeraraghavan, V. P., & Jayaraman, S. (2026) · Journal of Natural Remedies · doi:10.18311/jnr/2026/53621
Mello, B. (2016) · Springer eBooks · doi:10.1007/978-3-662-44324-8_458
Metadata
- Authors
- Pereira, A. P., Ferreira, I. C., Marcelino, F., Valentão, P., Andrade, P. B., Seabra, R., Estevinho, L., Bento, A., & Pereira, J. A.
- Affiliations
- Universidade Politécnica de Bragança (PT)
- REQUIMTE / Serviço de Farmacognosia, Faculdade de Farmácia da Universidade do Porto, Rua Aníbal Cunha, 164, 4050-047 Porto, Portugal
- Universidade do Porto (PT)
- CIMO / Escola Superior Agrária, Instituto Politécnico de Bragança, Campus Sta Apolónia, Apt. 1172, 5301-855 Bragança, Portugal
- Corresponding author
- Pereira, José Alberto
- Venue
- Molecules
- Publisher
- MDPI AG
- Published
- 2007-05-26
- Volume
- 12
- Issue
- 5
- Pages
- 1153-1162
- ISSN
- 1420-3049 (ISSN-L)
- PMC
- PMC6149345
- OpenAlex
- W2146146382
- Cited by
- 519
- References
- 26
- Citations by year
- 2026: 16 · 2025: 28 · 2024: 38 · 2023: 19 · 2022: 44 · 2021: 34 · 2020: 46 · 2019: 38 · 2018: 34 · 2017: 33 · 2016: 33 · 2015: 36 · 2014: 29 · 2013: 20 · 2012: 19
- FWCI
- 6.30
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Molecules (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Interreg
- Topics
- Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Health Sciences › Medicine › Biochemistry (medical) › Phytochemicals and Antioxidant Activities
- Keywords
- olive leaves, Olea europaea, phenolic compounds, antimicrobial activity, antibacterial activity, antifungal activity, caffeic acid, verbascoside, oleuropein, luteolin glycosides, rutin, apigenin glycosides, nutraceuticals, Bacillus cereus, Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, Candida albicans, Cryptococcus neoformans
- MeSH terms
- Anti-Infective Agents, Chromatography, High Pressure Liquid, Microbial Sensitivity Tests, Phenols, Molecular Structure, Plant Leaves, Olea
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
