Fibrillar Collagen Type I Participates in the Survival and Aggregation of Primary Hepatocytes Cultured on Soft Hydrogels
Open accessAbstract
Liver is an essential organ that carries out multiple functions such as glycogen storage, the synthesis of plasma proteins, and the detoxification of xenobiotics. Hepatocytes are the parenchyma that sustain almost all the functions supported by this organ. Hepatocytes and non-parenchymal cells respond to the mechanical alterations that occur in the extracellular matrix (ECM) caused by organogenesis and regenerating processes. Rearrangements of the ECM modify the composition and mechanical properties that result in specific dedifferentiation programs inside the hepatic cells. Quiescent hepatocytes are embedded in the soft ECM, which contains an important concentration of fibrillar collagens in combination with a basement membrane-associated matrix (BM). This work aims to evaluate the role of fibrillar collagens and BM on actin cytoskeleton organization and the function of rat primary hepatocytes cultured on soft elastic polyacrylamide hydrogels (PAA HGs). We used rat tail collagen type I and Matrigel® as references of fibrillar collagens and BM respectively and mixed different percentages of collagen type I in combination with BM. We also used peptides obtained from decellularized liver matrices (dECM). Remarkably, hepatocytes showed a poor adhesion in the absence of collagen on soft PAA HGs. We demonstrated that collagen type I inhibited apoptosis and activated extracellular signal-regulated kinases 1/2 (ERK1/2) in primary hepatocytes cultured on soft hydrogels. Epidermal growth factor (EGF) was not able to rescue cell viability in conjugated BM but affected cell aggregation in soft PAA HGs conjugated with combinations of different proportions of collagen and BM. Interestingly, actin cytoskeleton was localized and preserved close to plasma membrane (cortical actin) and proximal to intercellular ducts (canaliculi-like structures) in soft conditions; however, albumin protein expression was not preserved, even though primary hepatocytes did not remodel their actin cytoskeleton significantly in soft conditions. This investigation highlights the important role of fibrillar collagens on soft hydrogels for the maintenance of survival and aggregation of the hepatocytes. Data suggest evaluating the conditions that allow the establishment of optimal biomimetic environments for physiology and cell biology studies, where the phenotype of primary cells may be preserved for longer periods of time.
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Hepatocytes respond to increase of stiffness by enhancing actin contractility, downregulating HNF4A protein and activating ERK, PI3K and YAP signalling.
“Hepatocytes respond to increase of stiffness by enhancing actin contractility, downregulating HNF4A protein and activating extracellular signal‐regulated kinase (ERK), PI3K and YAP signalling [5, 22, 23].”
From Mechanical stiffness orchestrates distinct regulation of <scp>MRTFs</scp> and <scp>YAP</scp> / <scp>TAZ</scp> transcriptional cofactors in hepatocytes (2026) · cites this paper as [23]
Results §3.2 reports stress-fiber formation in hepatocytes on stiff hydrogels, consistent with increased actin contractility. Results §3.3 reports increased ERK1/2 activation on stiff hydrogels linked to a particular ECM mixture, not as a general response to stiffness. The searched passages do not establish HNF4A protein downregulation or PI3K activation; the Discussion mentions YAP/TAZ only as a likely contributor to fibrosis, not as a measured response to stiffness in these hepatocytes. (LLM, conf=0.88)
Experimental — accuracy not yet measuredThe most commonly applied ECM components for hepatocyte cultures are collagen type I and Matrigel.
“The most commonly applied ECM components for hepatocyte cultures are collagen type I and Matrigel, a commercially available BM matrix extract from Engelbreth-Holm-Swarm (EHS) mouse sarcoma (Serna-Márquez et al., 2020[35]; Zeilinger et al., 2016[49]).”
From Optimization of extracellular matrix for primary human hepatocyte cultures using mixed collagen-matrigel matrices (2023) · cites this paper as Serna-Márquez et al., 2020[35]
Serna-Márquez et al. reported a positive influence of collagen on viability and aggregation of rat hepatocytes cultured on soft conditions.
“Serna-Márquez et al. additionally examined the influence of different combinations of collagen type I and Matrigel conjugated to polyacrylamide hydrogels and reported a positive influence of collagen on viability and aggregation of rat hepatocytes cultured on soft conditions (Serna-Márquez et al., 2020[35]).”
From Optimization of extracellular matrix for primary human hepatocyte cultures using mixed collagen-matrigel matrices (2023) · cites this paper as Serna-Márquez et al., 2020[35]
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“…et al. additionally examined the influence of different combinations of collagen type I and Matrigel conjugated to polyacrylamide hydrogels and reported a positive influence of collagen on viability and aggregation of rat hepatocytes cultured on soft conditions (Serna-Márquez et al., 2020).”
InfluentialEngineering extracellular matrix-based liver hydrogels as advanced bio-mimetic platforms
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Research on Cartilage Repair and Biomimetic Applications of dECM Derived from Various Tissues
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- Authors
- Serna-Márquez, N., Rodríguez-Hernández, A., Ayala-Reyes, M., Martínez-Hernández, L. O., Peña-Rico, M. Á., Carretero-Ortega, J., Hautefeuille, M., & Vázquez-Victorio, G.
- Affiliations
- Laboratorio Nacional de Soluciones Biomiméticas para Diagnóstico y Terapia (LaNSBioDyT), Facultad de Ciencias, Universidad Nacional Autónoma de Mexico, Ciudad de México CP 04510, Mexico
- Universidad Nacional Autónoma de México (MX)
- Universidad del Papaloapan (MX)
- Venue
- Biomimetics
- Publisher
- MDPI AG
- Published
- 2020-06-25
- Volume
- 5
- Issue
- 2
- Pages
- 30
- ISSN
- 2313-7673 (ISSN-L)
- PMC
- PMC7345357
- OpenAlex
- W3037057528
- Cited by
- 15
- References
- 92
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 3 · 2022: 5 · 2021: 3
- FWCI
- 1.00
- Type
- journal article
- Language
- en
- Open access
- gold
- dx.doi.org
- Licence
- Locations
- Biomimetics (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Programa de Apoyo a Proyectos de Investigación e Innovación Tecnológica (PAPIIT) (IA206818)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- type I collagen, primary hepatocytes, soft hydrogels, polyacrylamide hydrogel, extracellular matrix, basement membrane, actin cytoskeleton, hepatocyte viability, apoptosis, ERK signaling, epidermal growth factor, albumin expression, cell adhesion
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