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Up-Regulation of microRNA* Strands by Their Target Transcripts
Abstract
During microRNA (miRNA) biogenesis, one strand of a 21–23 nucleotide RNA duplex is preferentially selected for entry into an RNA-induced silencing complex (RISC). The other strand, known as the miRNA* species, is typically thought to be degraded. Previous studies have provided miRNA* selection models, but it remains unclear how the dominance of one arm arises during the biogenesis of miRNA. Using miRNA sponge-like methods, we cloned four tandem target sequences (artificial target) of miR-7b* and then measured miR-7b* expression levels after transfection of the artificial target. miR-7b* levels were found to significantly increase after transfection of the artificial target. We postulate that the abundance of target transcripts drives miRNA arm selection.
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“It is noted there is a significant expression level difference between the guide strand miRNAs and passenger strand miRNAs (miRNA*) as researchers have previously noted [25,35,36,37].”
From Bioinformatic Interrogation of 5p-arm and 3p-arm Specific miRNA Expression Using TCGA Datasets (2015) · cites this paper as [35]
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Cited by
Sorted by citations
Bioinformatic Interrogation of 5p-arm and 3p-arm Specific miRNA Expression Using TCGA Datasets
Kuo, W.-T., Su, M.-W., Lee, Y., Chen, C.-H., Wu, C.-W., Fang, W.-L., Huang, K.-H., & Lin, W.-C. (2015) · Journal of Clinical Medicine · doi:10.3390/jcm4091798 · Cited by 30
Metadata
- Authors
- Kang, S.-M., Choi, J.-W., Hong, S.-H., & Lee, H.-J.
- Affiliations
- Department of Oral Microbiology, School of Dentistry, Kyungpook National University, Daegu 700-412, Korea
- ORCID
- Lee, Heon-Jin
- Venue
- International Journal of Molecular Sciences
- Publisher
- MDPI AG
- Published
- 2013-06-26
- Volume
- 14
- Issue
- 7
- Pages
- 13231-13240
- ISSN
- 1422-0067
- PMC
- PMC3742184
- Cited by
- 20
- References
- 0
- Language
- en
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