Cited by 111Nanoparticles: synthesis and applicationsGold and Silver Nanoparticles Synthesis and ApplicationsAntioxidant Activity and Oxidative Stress

The Effect of Silver Nanoparticles on Antioxidant/Pro-Oxidant Balance in a Murine Model

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Docea, Anca Oana, Calina, Daniela, Buga, Ana Maria, Zlatian, Ovidiu, Paoliello, M.M.B., Mogosanu, George Dan, Streba, Costin Teodor, Popescu, Elena Leocadia +5 more·2020·International Journal of Molecular Sciences

Abstract

This study aimed to evaluate the subacute effect of two types of Ag-NPs(EG-AgNPs and PVP-EG-AgNPs) on antioxidant/pro-oxidant balance in rats. Seventy Wistar rats (35 males and 35 females) were divided in 7 groups and intraperitoneally exposed for 28 days to 0, 1, 2 and 4 mg/kg bw/day EG-Ag-NPs and 1, 2 and 4 mg/kg bw/day PVP- EG-Ag-NPs. After 28 days, the blood was collected, and the total antioxidant capacity (TAC), thiobarbituric reactive species (TBARS),protein carbonyl (PROTC) levels, reduced glutathione (GSH) levels and catalase (CAT) activity were determined. EG-Ag-NPs determined protective antioxidant effects in a dose-dependent manner. The exposure to the 4 mg/kg bw/day EG-Ag-NPs determines both in males and females a significant increase in TAC and CAT and a significant decrease in TBARS and PROTC only in females. The PVP-EG-AgNPs showed a different trend compared to EG-AgNPs. At 4 mg/kg bw/day the PVP-EG-AgNPs induce increased PROTC levels and decreased GSH (males and females) and TAC levels (males). The different mechanisms of EG-AgNPs and PVP-EG-AgNPs on antioxidant-/pro-oxidant balance can be explained by the influence of coating agent used for the preparation of the nanoparticles in the formation and composition of protein corona that influence their pathophysiology in the organism.

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  1. [47]Messner D.J. Murray K.F. Kowdley K.V. Chapter 55—Mechanisms of Hepatocyte Detoxification Physiology of the Gastrointestinal Tract 5th ed. Johnson L.R. Ghishan F.K. Kaunitz J.D. Merchant J.L. Said H.M. Wood J.D. Academic Press Boston, MA, USA 2012 1507 1527

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Authors
Docea, A. O., Calina, D., Buga, A. M., Zlatian, O., Paoliello, M. M. B., Mogosanu, G. D., Streba, C. T., Popescu, E. L., Stoica, A. E., Bîrcă, A. C., Vasile, B. Ș., Grumezescu, A. M., & Mogoanta, L.
Affiliations
  • University of Medicine and Pharmacy of Craiova (RO)
  • Department of Clinical Pharmacy, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania
  • Department of Biochemistry, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania
  • Universidade Estadual de Londrina (BR)
  • Department of Pharmacognosy and Phytotherapy, Faculty of Pharmacy University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania
  • Universitatea Națională de Știință și Tehnologie Politehnica București (RO)
  • Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, Politehnica University of Bucharest, 011061 Bucharest, Romania
  • Department of Histology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania
Corresponding author
Docea, Anca Oana
Venue
International Journal of Molecular Sciences
Publisher
MDPI AG
Published
2020-02-12
Volume
21
Issue
4
Pages
1233
ISSN
1422-0067 (ISSN-L)
OpenAlex
W3006274881
Cited by
111
References
64
Citations by year
2026: 8 · 2025: 10 · 2024: 19 · 2023: 21 · 2022: 25 · 2021: 21 · 2020: 7
FWCI
7.19
Type
journal article
Language
en
Open access
Locations
  • International Journal of Molecular Sciences (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
Romanian National Authority for Scientific Research (45PCCDI/2018)
Topics
  • Physical Sciences › Materials Science › Materials Chemistry › Nanoparticles: synthesis and applications
  • Physical Sciences › Materials Science › Electronic, Optical and Magnetic Materials › Gold and Silver Nanoparticles Synthesis and Applications
  • Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
Keywords
silver nanoparticles, polyvinylpyrrolidone coating, protein corona, oxidative stress, lipid peroxidation, protein carbonylation, glutathione, catalase, total antioxidant capacity, subacute toxicity, sex differences
MeSH terms
Animals, Antioxidants, Disease Models, Animal, Female, Glutathione, Male, Particle Size, Silver, Rats, Wistar, Reactive Oxygen Species, Oxidative Stress, Rats, Metal Nanoparticles, Toxicity Tests, Subacute

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