Restraint Stress in Mice Alters Set of 25 miRNAs Which Regulate Stress- and Depression-Related mRNAs
Open accessAbstract
In the present study, we aim to identify the effect of restrain stress (RS) on the expression of miRNAs in mouse serum. We used three genotypes of animals (mice with knock-out of the gene-encoding norepinephrine transporter, NET-KO; C57BL/6J, and SWR/J) which had previously been shown to display different sensitivity to RS, and focused on miRNAs which were altered by RS in the serum of all three genotypes. An analysis of miRNAs expression allowed for the identification of a set of 25 differentially expressed miRNAs; 10 were down-regulated compared to an appropriate control group of animals, while 15 were up-regulated. The application of DIANA-miRPath v. 3.0 allowed for the identification of selected pathways (KEGG) and Gene Ontology (GO) categories that were significantly controlled by these miRNAs, while miRWalk v. 3.0—the platform that used the machine learning based algorithm, TaRPmiR—was used to find their targets. The results indicate that 25 miRNAs, identified as altered upon RS in three genotypes of mice, are responsible for regulation of mRNA-encoding proteins that are key for the main hypotheses of depression; therefore, they may help to understand the link between stress and depression at the molecular level.
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The link between miRNAs and the pathophysiology of depression may be implicated by the fact that miRNAs regulate neuronal regulation, neurotransmitter regulation, and microglia activation.
“The link between miRNAs and the pathophysiology of depression248–252 may be implicated by the fact that miRNAs regulate neuronal regulation, neurotransmitter regulation, and microglia activation.253,254”
From Epigenetic regulation in major depression and other stress-related disorders: molecular mechanisms, clinical relevance and therapeutic potential (2023) · cites this paper as [251]
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- Authors
- Solich, J., Kuśmider, M., Faron-Górecka, A., Pabian, P., & Dziedzicka-Wasylewska, M.
- Affiliations
- Maj Institute of Pharmacology (PL)
- Maj Institute of Pharmacology Polish Academy of Sciences, Smętna Street 12, 31-343 Kraków, Poland
- Corresponding author
- Solich, Joanna
- Venue
- International Journal of Molecular Sciences
- Publisher
- MDPI AG
- Published
- 2020-12-12
- Volume
- 21
- Issue
- 24
- Pages
- 9469
- ISSN
- 1422-0067 (ISSN-L)
- PMC
- PMC7763317
- OpenAlex
- W3111489577
- Cited by
- 13
- References
- 47
- Citations by year
- 2025: 2 · 2024: 4 · 2023: 2 · 2021: 5
- FWCI
- 0.60
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- International Journal of Molecular Sciences (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Science Centre Poland (2016/23/B/NZ4/01086)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
- Health Sciences › Medicine › Pharmacology › Treatment of Major Depression
- Keywords
- restraint stress, microRNA, serum microRNA expression, depression, stress-related genes, stress sensitivity, miRNA target prediction, mouse genotypes
- MeSH terms
- Machine Learning, Algorithms, Animals, Depression, Gene Expression Regulation, Mice, Inbred C57BL, Restraint, Physical, Stress, Physiological, Signal Transduction, Down-Regulation, Up-Regulation, Mice, Knockout, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, MicroRNAs, Norepinephrine Plasma Membrane Transport Proteins, Mice, Gene Regulatory Networks, Real-Time Polymerase Chain Reaction, Gene Ontology
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