CoChat
Sign Up
Cited by 195

In vitro genotoxicity of polystyrene nanoparticles on the human fibroblast Hs27 cell line

Poma, Anna, Vecchiotti, Giulia, Colafarina, Sabrina, Zarivi, Osvaldo, Aloisi, Massimo, Arrizza, Lorenzo, Chichiriccò, Giuseppe, Di Carlo, Piero·2019·Nanomaterials
DOI 10.3390/nano9091299PMC PMC6781270
Open this paper in CoChat — free account

Abstract

Several studies have provided information on environmental nanoplastic particles/debris, but the in vitro cyto-genotoxicity is still insufficiently characterized. The aim of this study is to analyze the effects of polystyrene nanoparticles (PNPs) in the Hs27 cell line. The viability of Hs27 cells was determined following exposure at different time windows and PNP concentrations. The genotoxic effects of the PNPs were evaluated by the cytokinesis-block micronucleus (CBMN) assay after exposure to PNPs. We performed ROS analysis on HS27 cells to detect reactive oxygen species at different times and treatments in the presence of PNPs alone and PNPs added to the Crocus sativus L. extract. The different parameters of the CBMN test showed DNA damage, resulting in the increased formation of micronuclei and nuclear buds. We noted a greater increase in ROS production in the short treatment times, in contrast, PNPs added to Crocus sativus extract showed the ability to reduce ROS production. Finally, the SEM-EDX analysis showed a three-dimensional structure of the PNPs with an elemental composition given by C and O. This work defines PNP toxicity resulting in DNA damage and underlines the emerging problem of polystyrene nanoparticles, which extends transversely from the environment to humans; further studies are needed to clarify the internalization process.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.3390/nano9091299

Cites

3 of 3 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Research works printed but not linked: 52

  1. [2]Andrady A.L. Microplastics in the marine environment Mar. Pollut. Bull. 2011 62 1596 1605 10.1016/j.marpolbul.2011.05.030 21742351 · DOI · Paper page
  2. [4]Ter Halle A. Jeanneau L. Martignac M. Jardé E. Pedrono B. Brach L. Gigault J. Nanoplastic in the North Atlantic subtropical gyre Environ. Sci. Technol. 2017 51 13689 13697 10.1021/acs.est.7b03667 29161030 · DOI · Paper page
  3. [5]Mattsson K. Jocic S. Doverbratt I. Hansson L.A. Nanoplastics in the aquatic environment Microplastic Contamination in Aquatic Environments Elsevier Amsterdam, The Netherlands 2018 379 399 10.1016/B978-0-12-813747-5.00013-8 · DOI
  4. [6]Browne M.A. Niven S.J. Galloway T.S. Rowland S.J. Thompson R.C. Microplastic moves pollutants and additives to worms, reducing functions linked to health and biodiversity Curr. Biol. 2013 23 2388 2392 10.1016/j.cub.2013.10.012 24309271 · DOI · Paper page
  5. [7]Della Torre C. Bergami E. Salvati A. Faleri C. Cirino P. Dawson K.A. Corsi I. Accumulation and embryotoxicity of polystyrene nanoparticles at early stage of development of sea urchin embryos Paracentrotus lividus Environ. Sci. Technol. 2014 48 12302 12311 10.1021/es502569w 25260196 · DOI · Paper page
  6. [8]Phuong N.N. Zalouk-Vergnoux A. Poirier L. Kamari A. Châtel A. Mouneyrac C. Lagarde F. Is there any consistency between the microplastics found in the field and those used in laboratory experiments? Environ. Pollut. 2016 211 111 123 10.1016/j.envpol.2015.12.035 26745396 · DOI · Paper page
  7. [9]Ma Y. Huang A. Cao S. Sun F. Wang L. Guo H. Ji R. Effects of nanoplastics and microplastics on toxicity, bioaccumulation, and environmental fate of phenanthrene in fresh water Environ. Pollut. 2016 219 166 173 10.1016/j.envpol.2016.10.061 27814532 · DOI · Paper page
  8. [10]Sussarellu R. Suquet M. Thomas Y. Lambert C. Fabioux C. Pernet M.E.J. Corporeau C. Oyster reproduction is affected by exposure to polystyrene microplastics Proc. Natl. Acad. Sci. USA 2016 113 2430 2435 10.1073/pnas.1519019113 26831072 PMC4780615 · DOI
  9. [11]Li J. Qu X. Su L. Zhang W. Yang D. Kolandhasamy P. Shi H. Microplastics in mussels along the coastal waters of China Environ. Pollut. 2016 214 177 184 10.1016/j.envpol.2016.04.012 27086073 · DOI
  10. [13]Cole M. Galloway T.S. Ingestion of nanoplastics and microplastics by Pacific oyster larvae Environ. Sci. Technol. 2015 49 14625 14632 10.1021/acs.est.5b04099 26580574 · DOI
  11. [14]Cole M. Lindeque P. Fileman E. Halsband C. Galloway T.S. The impact of polystyrene microplastics on feeding, function and fecundity in the marine copepod Calanus helgolandicus Environ. Sci. Technol. 2015 49 1130 1137 10.1021/es504525u 25563688 · DOI · Paper page
  12. [15]Gigault J. Ter Halle A. Baudrimont M. Pascal P.Y. Gauffre F. Phi T.L. Reynaud S. Current opinion: What is a nanoplastic? Environ. Pollut. 2018 235 1030 1034 10.1016/j.envpol.2018.01.024 29370948 · DOI · Paper page
  13. [16]Lambert S. Wagner M. Formation of microscopic particles during the degradation of different polymers Chemosphere 2016 161 510 517 10.1016/j.chemosphere.2016.07.042 27470943 · DOI
  14. [17]Alimi O.S. Farner Budarz J. Hernandez L.M. Tufenkji N. Microplastics and nanoplastics in aquatic environments: Aggregation, deposition, and enhanced contaminant transport Environ. Sci. Technol. 2018 52 1704 1724 10.1021/acs.est.7b05559 29265806 · DOI · Paper page
  15. [18]Bouwmeester H. Hollman P.C. Peters R.J. Potential health impact of environmentally released micro-and nanoplastics in the human food production chain: Experiences from nanotoxicology Environ. Sci. Technol. 2015 49 8932 8947 10.1021/acs.est.5b01090 26130306 · DOI
  16. [19]Verma A. Stellacci F. Effect of surface properties on nanoparticle–cell interactions Small 2010 6 12 21 10.1002/smll.200901158 19844908 · DOI
  17. [21]Ward J.E. Kach D.J. Marine aggregates facilitate ingestion of nanoparticles by suspension-feeding bivalves Mar. Environ. Res. 2009 68 137 142 10.1016/j.marenvres.2009.05.002 19525006 · DOI
  18. [22]Wegner A. Besseling E. Foekema E.M. Kamermans P. Koelmans A.A. Effects of nanopolystyrene on the feeding behavior of the blue mussel (Mytilus edulis L.) Environ. Toxic. Chem. 2012 31 2490 2497 10.1002/etc.1984 22893562 · DOI · Paper page
  19. [23]Bhattacharya P. Lin S.J. Turner J.P. Ke P.C. Physical adsorption of charged plastic nanoparticles affects algal photosynthesis J. Phys. Chem. C 2010 114 16556 16561 10.1021/jp1054759 · DOI · Paper page
  20. [24]Brillant M. MacDonald B. Postingestive selection in the sea scallop (Placopectenmagellanicus) on the basis of chemical properties of particles Mar. Biol. 2002 141 457 465 10.1007/s00227-002-0845-2 · DOI
  21. [25]Cedervall T. Hansson L.A. Lard M. Frohm B. Linse S. Food chain transport of nanoparticles affects behaviour and fat metabolism in fish PLoS ONE 2012 7 e32254 10.1371/journal.pone.0032254 22384193 PMC3284555 · DOI
  22. [26]Hussain N. Jani P.U. Florence A.T. Enhanced oral uptake of tomato lectin-conjugated nanoparticles in the rat Pharm. Res. 1997 14 613 618 10.1023/A:1012153011884 9165532 · DOI
  23. [27]Kulkarni S.A. Feng S.S. Effects of particle size and surface modification on cellular uptake and biodistribution of polymeric nanoparticles for drug delivery Pharm. Res. 2013 30 2512 2522 10.1007/s11095-012-0958-3 23314933 · DOI
  24. [28]Walczak A.P. Kramer E. Hendriksen P.J. Helsdingen R. van der Zande M. Rietjens I.M. Bouwmeester H. In vitro gastrointestinal digestion increases the translocation of polystyrene nanoparticles in an in vitro intestinal co-culture model Nanotoxicology 2015 9 886 894 10.3109/17435390.2014.988664 25672814 · DOI
  25. [29]EFSA Scientific Committee Guidance on the risk assessment of the application of nanoscience and nanotechnologies in the food and feed chain EFSA J. 2011 9 2140 10.2903/j.efsa.2011.2140 PMC7009542 32625968 · DOI
  26. [30]Saquib Q. Faisal M. Al-Khedhairy A.A. Alatar A.A. Cellular and Molecular Toxicology of Nanoparticles Springer New York, NY, USA 2018 Volume 1048 10.1007/978-3-319-72041-8 · DOI
  27. [31]Karlsson H.L. The comet assay in nanotoxicology research Anal. Bioanal. Chem. 2010 398 651 666 10.1007/s00216-010-3977-0 20640410 · DOI
  28. [32]Xie H. Mason M.M. Wise J.P. Genotoxicity of metal nanoparticles Rev. Environ. Health 2011 26 251 268 10.1515/REVEH.2011.033 22435324 · DOI
  29. [33]Hernandez L.M. Yousefi N. Tufenkji N. Are there nanoplastics in your personal care products? Environ. Sci. Technol. Lett. 2017 4 280 285 10.1021/acs.estlett.7b00187 · DOI · Paper page
  30. [34]Singh N. Manshian B. Jenkins G.J. Griffiths S.M. Williams P.M. Maffeis T.G. Doak S.H. NanoGenotoxicology: The DNA damaging potential of engineered nanomaterials Biomaterials 2009 30 3891 3914 10.1016/j.biomaterials.2009.04.009 19427031 · DOI
  31. [35]Anderson A.G. Grose J. Pahl S. Thompson R.C. Wyles K.J. Microplastics in personal care products: Exploring perceptions of environmentalists, beauticians and students Marine Pollut. Bullet. 2016 113 454 460 10.1016/j.marpolbul.2016.10.048 27836135 · DOI
  32. [36]Menghini L. Leporini L. Vecchiotti G. Locatelli M. Carradori S. Ferrante C. Brunetti L. Crocus sativus L. stigmas and byproducts: Qualitative fingerprint, antioxidant potentials and enzyme inhibitory activities Food Res. Int. 2018 109 91 98 10.1016/j.foodres.2018.04.028 29803496 · DOI
  33. [37]Lahmass I. Lamkami T. Delporte C. Sikdar S. Van Antwerpen P. Saalaoui E. Megalizzi V. The waste of saffron crop, a cheap source of bioactive compounds J. Funct. Foods 2017 35 341 351 10.1016/j.jff.2017.05.057 · DOI
  34. [38]Cory A.H. Owen T.C. Barltrop J.A. Cory J.G. Use of an aqueous soluble tetrazolium/formazan assay for cell growth assays in culture Cancer Commun. 1991 3 207 212 10.3727/095535491820873191 1867954 · DOI
  35. [39]Fenech M. Cytokinesis-block micronucleus cytome assay Nat. Protoc. 2007 2 1084 1104 10.1038/nprot.2007.77 17546000 · DOI
  36. [40]OECD Guidelines for the Testing of Chemicals, Section 4: Health Effects. Test No. 487. Vitro Mammalian Cell Micronucleus Test Available online: www.oecd-ilibrary.org (accessed on 4 September 2019)
  37. [41]Andrady A.L. Neal M.A. Applications and Societal Benefits of Plastics Philos. Trans. R. Soc. Lond. Ser. B 2009 364 1977 1984 10.1098/rstb.2008.0304 19528050 PMC2873019 · DOI · Paper page
  38. [42]Jambeck J.R. Geyer R. Wilcox C. Siegler T.R. Perryman M. Andrady A. Narayan R. Law K.L. Marine pollution. Plastic waste inputs from land into the ocean Science 2015 347 768 771 10.1126/science.1260352 25678662 · DOI · Paper page
  39. [43]Wilcox C. Van Sebille E. Hardesty B.D. Threat of plastic pollution to seabirds is global, pervasive, and increasing Proc. Natl. Acad. Sci. USA 2015 112 11899 11904 10.1073/pnas.1502108112 26324886 PMC4586823 · DOI
  40. [44]Gregory M.R. Environmental implications of plastic debris in marine settings—Entanglement, ingestion, smothering, hangers-on, hitch-hiking and alien invasions Philos. Trans. R. Soc. Lond. Ser. B Biol. Sci. 2009 364 2013 2025 10.1098/rstb.2008.0265 19528053 PMC2873013 · DOI · Paper page
  41. [45]Mrosovsky N. Ryan G.D. James M.C. Leatherback turtles: The menace of plastic Mar. Pollut. Bull. 2009 58 287 289 10.1016/j.marpolbul.2008.10.018 19135688 · DOI
  42. [46]Laist D.W. Impacts of marine debris: Entanglement of marine life in marine debris including a comprehensive list of species with entanglement and ingestion records Marine Debris Springer New York, NY, USA 1997 99 139 10.1007/978-1-4613-8486-1_10 · DOI
  43. [47]Mishra P. Vinayagam S. Duraisamy K. Patil S.R. Godbole J. Mohan A. Chandrasekaran N. Distinctive impact of polystyrene nano-spherules as an emergent pollutant toward the environment Environ. Sci. Poll. Res. 2019 26 1537 1547 10.1007/s11356-018-3698-z 30430449 · DOI
  44. [48]Bukhari S.I. Manzoor M. Dhar M.K. A comprehensive review of the pharmacological potential of Crocus sativus and its bioactive apocarotenoids Biomed. Pharmacother. 2018 98 733 745 10.1016/j.biopha.2017.12.090 29306211 · DOI
  45. [49]Frankel E.N. Nutritional benefits of flavonoids Food Factors for Cancer Prevention Springer Tokyo, Japan 1997 613 616 10.1007/978-4-431-67017-9_121 · DOI
  46. [50]Xia T. Kovochich M. Brant J. Hotze M. Sempf J. Oberley T. Nel A.E. Comparison of the abilities of ambient and manufactured nanoparticles to induce cellular toxicity according to an oxidative stress paradigm Nano Lett. 2006 6 1794 1807 10.1021/nl061025k 16895376 · DOI
  47. [51]Rabinovitch M. Professional and non-professional phagocytes: An introduction Trends Cell Biol. 1995 5 85 87 10.1016/S0962-8924(00)88955-2 14732160 · DOI
  48. [52]Rodriguez-Seijo A. Lourenço J. Rocha-Santos T.A.P. Da Costa J. Duarte A.C. Vala H. Pereira R. Histopathological and molecular effects of microplastics in Eisenia andrei Bouché Environ. Pollut. 2017 220 495 503 10.1016/j.envpol.2016.09.092 27745914 · DOI
  49. [53]Pedà C. Caccamo L. Fossi M.C. Gai F. Andaloro F. Genovese L. Maricchiolo G. Intestinal alterations in European sea bass Dicentrarchus labrax (Linnaeus, 1758) exposed to microplastics: Preliminary results Environ. Pollut. 2016 212 251 256 10.1016/j.envpol.2016.01.083 26851981 · DOI · Paper page
  50. [54]Chua E.M. Shimeta J. Nugegoda D. Morrison P.D. Clarke B.O. Assimilation of polybrominated diphenyl ethers from microplastics by the marine amphipod, Allorchestes compressa Environ. Sci. Technol. 2014 48 8127 8134 10.1021/es405717z 24884099 · DOI · Paper page
  51. [55]Koelmans A.A. Besseling E. Foekema E.M. Leaching of plastic additives to marine organisms Environ. Pollut. 2014 187 49 54 10.1016/j.envpol.2013.12.013 24440692 · DOI
  52. [56]Nowack B. Ranville J.F. Diamond S. Gallego-Urrea J.A. Metcalfe C. Rose J. Horne N. Koelmans A.A. Klaine S.J. Potential scenarios for nanomaterial release and subsequent alteration in the environment Environ. Toxicol. Chem. 2012 31 50 59 10.1002/etc.726 22038832 · DOI

Cited by

Sorted by citations

Authors
Poma, A., Vecchiotti, G., Colafarina, S., Zarivi, O., Aloisi, M., Arrizza, L., Chichiriccò, G., & Di Carlo, P.
Affiliations
  • Department of Life, Health and Environmental Sciences, University of L’Aquila, I-67100 L’Aquila, Italy
  • Center for Microscopy, University of L’Aquila, I-67100 L’Aquila, Italy
  • Department of Psychological, Health & Territorial Sciences, University “G. d’Annunzio” of Chieti-Pescara, I-66100 Chieti, Italy
ORCID
Venue
Nanomaterials
Publisher
MDPI AG
Published
2019-09-11
Volume
9
Issue
9
Pages
1299
ISSN
2079-4991
DOI
10.3390/nano9091299
PMC
PMC6781270
Cited by
195
References
3
Language
en
Licence
creativecommons.org/licenses/by/4.0/ (vor)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review