Protective effects of tyndallized Lactobacillus�acidophilus IDCC 3302 against UVB‑induced photodamage to epidermal keratinocytes cells
Open accessAbstract
Photoaging is a consequence of chronic exposure to ultraviolet (UV) radiation and results in skin damage. In this study, whether tyndallizate of the probiotic bacterium Lactobacillus acidophilus IDCC 3302 (ACT 3302) can protect against UVB‑induced photodamage to the skin was investigated. For this, HaCaT keratinocytes were used as a model for skin photoaging. HaCaT cells were treated with ACT 3302 prior to UVB exposure and skin hydration factors and matrix metalloproteinase (MMP)‑1, MMP‑2, and MMP‑9 levels in the culture supernatant were evaluated by ELISA. The protective effects of ACT 3302 against UVB‑induced oxidative stress in HaCaT cells was also assessed by measuring superoxide dismutase and catalase activity and detecting the expression of pro‑inflammatory cytokine‑encoding genes and mitogen‑activated protein kinase (MAPK) signaling components by reverse transcription‑quantitative polymerase chain reaction and western blotting, respectively. UVB exposure increased MMP expression and MAPK activation; these changes were attenuated by pretreatment with ACT 3302. Treatment with ACT 3302 prior to UVB exposure also attenuated inflammation. These results demonstrate that tyndallized ACT 3302 can mitigate photodamage to the skin induced by UVB radiation through the suppression of MMPs and could therefore be used clinically to prevent wrinkle formation.
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The protective mechanism is consistent with previous studies showing that heat-killed Lactobacillus strains exert anti-photoaging effects through modulation of the MAPK/MMP-1 pathway.
“The protective mechanism is consistent with previous studies showing that heat-killed Lactobacillus strains exert anti-photoaging effects through modulation of the MAPK/MMP-1 pathway [22].”
From Anti-Photoaging and Anti-Melanogenic Effects of a Novel <i>Lactiplantibacillus plantarum</i> RKBP Isolated from Giant <i>Centella asiatica</i> (2026) · cites this paper as [22]
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- Authors
- Im, A., Lee, B., Kang, D., & Chae, S.
- Affiliations
- Korea Institute of Oriental Medicine (KR)
- Ildong (South Korea) (KR)
- Venue
- International Journal of Molecular Medicine
- Publisher
- Spandidos Publications
- Published
- 2019-04-10
- Volume
- 43
- Issue
- 6
- Pages
- 2499-2506
- ISSN
- 1107-3756 (ISSN-L)
- 1791-244X
- OpenAlex
- W2938219671
- Cited by
- 32
- References
- 43
- Citations by year
- 2026: 2 · 2025: 6 · 2024: 6 · 2023: 6 · 2022: 6 · 2021: 5 · 2020: 1
- FWCI
- 3.05
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- International Journal of Molecular Medicine (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Korea Institute of Oriental Medicine (K17300)
- Topics
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
- Keywords
- Lactobacillus acidophilus, photoaging, epidermal keratinocytes, HaCaT keratinocytes, matrix metalloproteinases, oxidative stress, MAPK signaling, inflammatory cytokines, skin hydration, wrinkle prevention, probiotics
- MeSH terms
- Cell Line, Cell Survival, Humans, Lactobacillus acidophilus, Ultraviolet Rays, Skin Aging, Keratinocytes, Oxidative Stress, Probiotics, Protective Factors
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