Cited by 241Neuroscience and Neuropharmacology ResearchRegulation of Appetite and ObesityAmino Acid Enzymes and Metabolism

Distribution of vesicular glutamate transporter mRNA in rat hypothalamus

Ziegler, Dana R., Cullinan, William E., Herman, James P.·2002·Journal of Comparative Neurology

Abstract

Two isoforms of the vesicular glutamate transporter, VGLUT1 and VGLUT2, were recently cloned and biophysically characterized. Both VGLUT1 and VGLUT2 specifically transport glutamate into synaptic vesicles, making them definitive markers for neurons using glutamate as a neurotransmitter. The present study takes advantage of the specificity of the vesicular transporters to afford the first detailed map of putative glutamatergic neurons in the rat hypothalamus. In situ hybridization analysis was used to map hypothalamic distributions of VGLUT1 and VGLUT2 mRNAs. VGLUT2 is clearly the predominant vesicular transporter mRNA found in the hypothalamus; rich expression can be documented in regions regulating energy balance (ventromedial hypothalamus), neuroendocrine function (preoptic nuclei), autonomic tone (posterior hypothalamus), and behavioral/homeostatic integration (lateral hypothalamus, mammillary nuclei). Expression of VGLUT1 is decidedly more circumspect and is confined to relatively weak labeling in lateral hypothalamic regions, neuroendocrine nuclei, and the suprachiasmatic nucleus. Importantly, dual-label analysis revealed no incidence of colocalization of VGLUT1 or VGLUT2 mRNAs in glutamic acid decarboxylase (GAD) 65-positive neurons, indicating that GABA neurons do not express either transporter. Our data support a major role for hypothalamic glutamatergic neurons in regulation of all aspects of hypothalamic function.

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Crossref · 10.1002/cne.10257

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Authors
Ziegler, D. R., Cullinan, W. E., & Herman, J. P.
Affiliations
  • University of Kentucky (US)
  • Marquette University (US)
Corresponding author
Herman, James P.
Venue
Journal of Comparative Neurology
Publisher
Wiley
Published
2002-05-21
Volume
448
Issue
3
Pages
217-229
ISSN
  • 0021-9967 (ISSN-L)
  • 1096-9861
OpenAlex
W1977741133
Cited by
241
References
53
Citations by year
2026: 1 · 2025: 1 · 2024: 4 · 2023: 10 · 2022: 8 · 2021: 10 · 2020: 5 · 2019: 12 · 2018: 14 · 2017: 10 · 2016: 11 · 2015: 11 · 2014: 9 · 2013: 12 · 2012: 9
FWCI
5.25
Type
journal article
Language
en
Open access
closed
Locations
  • The Journal of Comparative Neurology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r29mh056577-05, 5f31mh011980-02, 5r03mh060819-03)
  • National Institute of Mental Health (MH 60819, MH49698, MH56577, MH 11980)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
Keywords
vesicular glutamate transporter, VGLUT1, VGLUT2, hypothalamus, glutamatergic neurons, glutamatergic neurotransmission, mRNA distribution, ventromedial hypothalamus, suprachiasmatic nucleus, lateral hypothalamus, mammillary nuclei, GABAergic neurons, energy balance, neuroendocrine function, autonomic regulation
MeSH terms
Animals, Carrier Proteins, Gene Expression Regulation, Glutamate Decarboxylase, Hypothalamus, Male, Synaptic Transmission, Neurons, RNA, Messenger, Synaptic Vesicles, Biomarkers, Rats, Sprague-Dawley, In Situ Hybridization, Glutamic Acid, Membrane Transport Proteins, Vesicular Transport Proteins, Vesicular Glutamate Transport Protein 1, Vesicular Glutamate Transport Protein 2, Rats

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