Cited by 202Alzheimer's disease research and treatmentsDementia and Cognitive Impairment ResearchNeuroinflammation and Neurodegeneration Mechanisms

Amyloid β Protein Starting Pyroglutamate at Position 3 Is a Major Component of the Amyloid Deposits in the Alzheimer's Disease Brain

Harigaya, Yasuo, Saido, Takaomi C., Eckman, Christopher B., Prada, Cristian-Mihail, Shoji, Mikio, Younkin, Steven G.·2000·Biochemical and Biophysical Research Communications

Abstract

The amyloid beta protein (Abeta) deposited in the Alzheimer's disease (AD) brain is heterogeneous at both its amino and carboxyl termini. Recent studies of the genetic forms of AD indicate that the aggregation and deposition of Abeta42 may be a common initiating event in all forms of AD. Here, we analyzed the amino termini of the Abeta species deposited in the AD brain, focusing specifically on species with amino-terminal pyroglutamate at position 3 (Abeta3(pE)). Immunocytochemical analysis of AD brains with an antibody specific for Abeta3(pE) confirmed that these species deposit in blood vessels and senile plaques. Using specific sandwich ELISAs, we determined the amounts of Abeta3(pE)-40 and Abeta3(pE)-42(43) in AD brain compared with other forms. This analysis showed that Abeta3(pE)-40 is closely correlated with the extent of Abeta deposition in blood vessels, whereas Abeta3(pE)-42(43) is not. In addition, Abeta3(pE)-42(43) is an important component of the Abeta deposited in senile plaques of the AD brain, constituting approximately 25% of the total Abeta42(43). In vitro comparison of Abeta1-42 and Abeta3(pE)-42 showed that Abeta3(pE)-42 is highly prone to oligomerization. These findings suggest that Abeta3(pE)-42 may be particularly important in AD pathogenesis.

Integrity signals

Registry

Crossref · 10.1006/bbrc.2000.3490

Cites

30 of 30 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Harigaya, Y., Saido, T. C., Eckman, C. B., Prada, C.-M., Shoji, M., & Younkin, S. G.
Affiliations
  • Mayo Clinic (US)
  • RIKEN Center for Brain Science (JP)
  • Gunma University (JP)
Corresponding author
Harigaya, Yasuo
Venue
Biochemical and Biophysical Research Communications
Publisher
Elsevier BV
Published
2000-09
Volume
276
Issue
2
Pages
422-427
ISSN
0006-291X (ISSN-L)
OpenAlex
W2027693753
Cited by
202
References
30
Citations by year
2026: 4 · 2025: 4 · 2024: 7 · 2023: 11 · 2022: 5 · 2021: 15 · 2020: 10 · 2019: 11 · 2018: 7 · 2017: 15 · 2016: 8 · 2015: 7 · 2014: 11 · 2013: 9 · 2012: 14
FWCI
0.61
Type
journal article
Language
en
Open access
closed
Locations
  • Biochemical and Biophysical Research Communications (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Univers Foundation
  • Ministry of Education, Culture, Sports, Science and Technology (10832002, 10832003)
  • Ministry of Health, Labour and Welfare
  • MSD Life Science Foundation, Public Interest Incorporated Foundation
  • Uehara Memorial Foundation
Topics
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
  • Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
Keywords
amyloid-beta, Alzheimer's disease, amyloid deposits, amyloid plaques, amyloid-beta 42, Aβ40, oligomerization, amyloid deposition, Alzheimer's disease pathogenesis
MeSH terms
Aged, Aged, 80 and over, Alzheimer Disease, Brain, Female, Humans, Male, Peptide Fragments, Pyrrolidonecarboxylic Acid, Amyloid beta-Peptides, Plaque, Amyloid

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review