Microbiome dynamics during ensiling of corn with and without Lactobacillus plantarum inoculant

Keshri, Jitendra; Chen, Yaira; Pinto, Riky; Kroupitski, Yulia; Weinberg, Zwi G.; Sela, Shlomo

doi:10.1007/s00253-018-8903-y

Cited by 210Ruminant Nutrition and Digestive PhysiologyAnimal Nutrition and PhysiologyMycotoxins in Agriculture and Food

Microbiome dynamics during ensiling of corn with and without Lactobacillus plantarum inoculant

Keshri, Jitendra, Chen, Yaira, Pinto, Riky, Kroupitski, Yulia, Weinberg, Zwi G., Sela, Shlomo·2018·Applied Microbiology and Biotechnology

DOI 10.1007/s00253-018-8903-y

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Abstract

Microbial population dynamics associated with corn silage, with and without Lactobacillus plantarum treatment, was studied. Whole crop corn was ensiled using laboratory silos and sampled at different times, up to 3 months. The dominant bacteria, before ensiling, were Acinetobacter (38.5%) and Klebsiella (16.3%), while the dominant fungi were Meyerozyma (53.5%) and Candida (27.7%). During ensiling, the microbial population shifted considerably, and Lactobacillus (> 94%) and Candida (> 74%) became the most dominant microbial genera in both treated and untreated silages. Yet, lactic acid content was higher in the treated silage, while the microbial diversity was lower than in the untreated silage. Upon aerobic exposure, spoilage occurred more rapidly in the treated silage, possibly due to the higher abundance of lactic acid-assimilating fungi, such as Candida. Our study is the first to describe microbial population dynamics during whole-crop corn ensiling and the results indicate that microbial diversity may be an indicator of aerobic stability.

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Our results after 1 day of incubation are in line with the diversity reported at time 0 by Gharechahi et al. and to data after 1 and 2 days from Keshri et al.
“Our results after 1 day of incubation are in line with the diversity reported at time 0 by Gharechahi et al. [35] and to data after 1 and 2 days from Keshri et al. [39].”
From Dynamic succession of microbiota during ensiling of whole plant corn following inoculation with Lactobacillus buchneri and Lactobacillus hilgardii alone or in combination. (2019) · cites this paper as [39]
Keshri et al. observed similar phases of colonization and bacterial richness evolution in corn silage.
“Keshri et al. [39] observed similar phases of colonization and of bacterial richness evolution.”
From Dynamic succession of microbiota during ensiling of whole plant corn following inoculation with Lactobacillus buchneri and Lactobacillus hilgardii alone or in combination. (2019) · cites this paper as [39]
Keshri et al. compared a non-inoculated control to silage inoculated with a L. plantarum strain, but had the same interactions between Leuconostocaceae and Lactobacillaceae in relation with time of fermentation.
“They compared a non-inoculated control to silage, inoculated with a L. plantarum strain, but had the same interactions between Leuconostocaceae and Lactobacillaceae in relation with time of fermentation.”
From Dynamic succession of microbiota during ensiling of whole plant corn following inoculation with Lactobacillus buchneri and Lactobacillus hilgardii alone or in combination. (2019) · cites this paper as [39]

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Authors: Keshri, J., Chen, Y., Pinto, R., Kroupitski, Y., Weinberg, Z. G., & Sela, S.
Affiliations: Agricultural Research Organization (IL)
Corresponding author: Sela, Shlomo
ORCID: Keshri, Jitendra
Weinberg, Zwi G.
Sela, Shlomo
Venue: Applied Microbiology and Biotechnology
Publisher: Springer Science and Business Media LLC
Published: 2018-03-13
Volume: 102
Issue: 9
Pages: 4025-4037
ISSN: 0175-7598 (ISSN-L)
1432-0614
DOI: 10.1007/s00253-018-8903-y
OpenAlex: W2794163481
Cited by: 210
References: 47
Citations by year: 2026: 14 · 2025: 20 · 2024: 23 · 2023: 36 · 2022: 46 · 2021: 37 · 2020: 22 · 2019: 10 · 2018: 2
FWCI: 29.99
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Applied Microbiology and Biotechnology (publishedVersion)
PubMed (publishedVersion)
Funders: United States - Israel Binational Agricultural Research and Development Fund (IS-4704-14)
EU COST Action FA1202 (CGAFA1202)
Topics: Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
Life Sciences › Agricultural and Biological Sciences › Plant Science › Mycotoxins in Agriculture and Food
Keywords: corn silage, silage inoculation, microbial population dynamics, microbial diversity, lactic acid, aerobic stability, silage spoilage, Candida, Acinetobacter, Klebsiella, Meyerozyma
MeSH terms: Aerobiosis, Agriculture, Zea mays, Fermentation, Silage, Lactiplantibacillus plantarum, Lactobacillus plantarum, Microbiota

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Microbiome dynamics during ensiling of corn with and without Lactobacillus plantarum inoculant

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