Cited by 60Yeasts and Rust Fungi StudiesRuminant Nutrition and Digestive PhysiologyPlant Pathogens and Fungal Diseases

Identification of the major yeasts isolated from high moisture corn and corn silages in the United States using genetic and biochemical methods

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Santos, M.C., Golt, C., Joerger, R.D., Mechor, G.D., Mourão, Gerson B., Kung, L.·2017·Journal of Dairy Science

Abstract

The objective of this study was to identify species of yeasts in samples of high moisture corn (HMC) and corn silage (CS) collected from farms throughout the United States. Samples were plated and colonies were isolated for identification using DNA analysis. Randomly selected colonies were also identified by fatty acid methyl esters (FAME) and by physiological substrate profiling (ID 32C). For CS, Candida ethanolica, Saccharomyces bulderi, Pichia anomala, Kazachstania unispora, and Saccharomyces cerevisiae were the predominant yeasts. Pichia anomala, Issatchenkia orientalis, S. cerevisiae, and Pichia fermentans were the prevalent species in HMC. The 3 identification methods were in agreement at the species level for 16.6% of the isolates and showed no agreement for 25.7%. Agreement in species identification between ID 32C and DNA analysis, FAME and ID 32C, and FAME and DNA analysis was 41.1, 14.4, and 2.2%, respectively. Pichia anomala and I. orientalis were able to grow on lactic acid, whereas S. cerevisiae metabolized sugars (galactose, sucrose, and glucose) but failed to use lactic acid. The yeast diversity in CS and HMC varied due to type of feed and location. Differences in species assignments were seen among methods, but identification using substrate profiling generally corresponded with that based on DNA analysis. These findings provide information about the species that may be expected in silages, and this knowledge may lead to interventions that control unwanted yeasts.

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Crossref · 10.3168/jds.2016-11450

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Authors
Santos, M. C., Golt, C., Joerger, R. D., Mechor, G. D., Mourão, G. B., & Kung, L.
Affiliations
  • University of Delaware (US)
  • Elanco (United States) (US)
Corresponding author
Kung, L.
Venue
Journal of Dairy Science
Published
2017-02
Volume
100
Issue
2
Pages
1151-1160
ISSN
0022-0302 (ISSN-L)
OpenAlex
W2554366902
Cited by
60
References
47
Citations by year
2026: 3 · 2025: 8 · 2024: 6 · 2023: 6 · 2022: 11 · 2021: 5 · 2020: 8 · 2019: 9 · 2018: 2 · 2017: 2
FWCI
2.25
Type
journal article
Language
en
Open access
Locations
  • Journal of Dairy Science (publishedVersion)
  • PubMed (publishedVersion)
Funders
Elanco Animal Health
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
  • Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Plant Pathogens and Fungal Diseases
Keywords
yeast identification, high-moisture corn, corn silage, Wickerhamomyces anomalus, Candida krusei, Saccharomyces cerevisiae, Candida ethanolica, Kazachstania unispora, Pichia fermentans, fatty acid methyl esters, substrate profiling, DNA-based identification, yeast diversity
MeSH terms
Animals, Zea mays, Saccharomycetales, Fermentation, Saccharomyces cerevisiae, Silage, United States, Yeasts

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