A long-term study on female mice fed on a genetically modified soybean: effects on liver ageing
Open accessAbstract
Liver represents a suitable model for monitoring the effects of a diet, due to its key role in controlling the whole metabolism. Although no direct evidence has been reported so far that genetically modified (GM) food may affect health, previous studies on hepatocytes from young female mice fed on GM soybean demonstrated nuclear modifications involving transcription and splicing pathways. In this study, the effects of this diet were studied on liver of old female mice in order to elucidate possible interference with ageing. The morpho-functional characteristics of the liver of 24-month-old mice, fed from weaning on control or GM soybean, were investigated by combining a proteomic approach with ultrastructural, morphometrical and immunoelectron microscopical analyses. Several proteins belonging to hepatocyte metabolism, stress response, calcium signalling and mitochondria were differentially expressed in GM-fed mice, indicating a more marked expression of senescence markers in comparison to controls. Moreover, hepatocytes of GM-fed mice showed mitochondrial and nuclear modifications indicative of reduced metabolic rate. This study demonstrates that GM soybean intake can influence some liver features during ageing and, although the mechanisms remain unknown, underlines the importance to investigate the long-term consequences of GM-diets and the potential synergistic effects with ageing, xenobiotics and/or stress conditions.
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- Authors
- Malatesta, M., Boraldi, F., Annovi, G., Baldelli, B., Battistelli, S., Biggiogera, M., & Quaglino, D.
- Affiliations
- University of Verona (IT)
- University of Modena and Reggio Emilia (IT)
- University of Urbino (IT)
- University of Pavia (IT)
- Corresponding author
- Malatesta, Manuela
- Venue
- Histochemistry and Cell Biology
- Published
- 2008-07-22
- Volume
- 130
- Issue
- 5
- Pages
- 967-977
- ISSN
- 0948-6143 (ISSN-L)
- 1432-119X
- OpenAlex
- W2167443257
- Cited by
- 88
- References
- 71
- Citations by year
- 2024: 2 · 2023: 2 · 2022: 12 · 2021: 3 · 2020: 2 · 2019: 3 · 2018: 6 · 2017: 2 · 2016: 8 · 2015: 8 · 2014: 8 · 2013: 10 · 2012: 7
- FWCI
- 6.05
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- Locations
- Histochemistry and Cell Biology (publishedVersion)
- PubMed (publishedVersion)
- IRIS UNIMORE (University of Modena and Reggio Emilia) (submittedVersion, cc-by)
- CINECA IRIS Institutional Research information system (University of Urbino) (submittedVersion)
- RUNG (submittedVersion, cc-by-nc-nd)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
- Keywords
- transgenic soybean, hepatocytes, senescence markers, mitochondrial dysfunction, calcium signaling, stress response, hepatocyte metabolism, ultrastructure, proteomics, female mice, long-term dietary effects
- MeSH terms
- Age Factors, Aging, Animals, Animal Feed, Animal Nutritional Physiological Phenomena, Female, Immunohistochemistry, Liver, Proteins, Glycine max, Microscopy, Immunoelectron, Plants, Genetically Modified, Food, Genetically Modified, Proteomics, Cell Size, Mice, Cell Nucleus Shape
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