Training in the fasted state facilitates re-activation of eEF2 activity during recovery from endurance exercise
Open accessAbstract
Nutrition is an important co-factor in exercise-induced training adaptations in muscle. We compared the effect of 6 weeks endurance training (3 days/week, 1-2 h at 75% VO(2peak)) in either the fasted state (F; n = 10) or in the high carbohydrate state (CHO, n = 10), on Ca(2+)-dependent intramyocellular signalling in young male volunteers. Subjects in CHO received a carbohydrate-rich breakfast before each training session, as well as ingested carbohydrates during exercise. Before (pretest) and after (posttest) the training period, subjects performed a 2 h constant-load exercise bout (~70% of pretest VO(2peak)) while ingesting carbohydrates (1 g/kg h(-1)). A muscle biopsy was taken from m. vastus lateralis immediately before and after the test, and after 4 h of recovery. Compared with pretest, in the posttest basal eukaryotic elongation factor 2 (eEF2) phosphorylation was elevated in CHO (P < 0.05), but not in F. In the pretest, exercise increased the degree of eEF2 phosphorylation about twofold (P < 0.05), and values returned to baseline within the 4 h recovery period in each group. However, in the posttest dephosphorylation of eEF2 was negated after recovery in CHO, but not in F. Independent of the dietary condition training enhanced the basal phosphorylation status of Phospholamban at Thr(17), 5'-AMP-activated protein kinase α (AMPKα), and Acetyl CoA carboxylase β (ACCβ), and abolished the exercise-induced increase of AMPKα and ACCβ (P < 0.05). In conclusion, training in the fasted state, compared with identical training with ample carbohydrate intake, facilitates post-exercise dephosphorylation of eEF2. This may contribute to rapid re-activation of muscle protein translation following endurance exercise.
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Van Proeyen et al. found no differences in AMPK in subjects training in the fasted vs. fed state, but did observe differences in post-exercise eukaryotic elongation factor 2 phosphorylation.
“Van Proeyen et al. [36] found no differences in AMPK in subjects training in the fasted vs. fed state, but did observe differences in post-exercise eukaryotic elongation factor 2 phosphorylation (elevated after carbohydrate feeding but not after fasting).”
From Periodized Nutrition for Athletes (2017) · cites this paper as [36]
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- Authors
- Van Proeyen, K., De Bock, K., & Hespel, P.
- Affiliations
- KU Leuven (BE)
- Corresponding author
- Hespel, P.
- ORCID
- Hespel, P.
- Venue
- European Journal of Applied Physiology
- Published
- 2010-12-04
- Volume
- 111
- Issue
- 7
- Pages
- 1297-1305
- ISSN
- 0020-9376 (ISSN-L)
- 1439-6319
- 1439-6327
- OpenAlex
- W1964075121
- Cited by
- 24
- References
- 63
- Citations by year
- 2026: 1 · 2024: 2 · 2022: 1 · 2021: 2 · 2019: 3 · 2018: 1 · 2017: 1 · 2016: 3 · 2015: 3 · 2014: 2 · 2013: 2 · 2012: 1
- FWCI
- 0.70
- Type
- journal article
- Language
- en
- Open access
- green
- lirias.kuleuven.be
- Licence
- other-oa
- www.springer.com/tdm (tdm)
- Locations
- European Journal of Applied Physiology (publishedVersion)
- PubMed (publishedVersion)
- Lirias (submittedVersion, other-oa)
- Funders
- KU Leuven
- Vlaamse regering
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Sports Performance and Training
- Keywords
- fasting state, endurance training, eEF2 phosphorylation, post-exercise recovery, carbohydrate intake, AMPK, acetyl-CoA carboxylase, phospholamban, calcium signaling, vastus lateralis, sports nutrition
- MeSH terms
- Adult, Dietary Carbohydrates, Enzyme Activation, Exercise Test, Fasting, Humans, Male, Phosphorylation, Physical Education and Training, Physical Endurance, Exercise, Recovery of Function, Peptide Elongation Factor 2, Elongation Factor 2 Kinase, Young Adult
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