The effects of acute aerobic and resistance exercise on mTOR signaling and autophagy markers in untrained human skeletal muscle
Open accessAbstract
Purpose Aerobic (AE) and resistance (RE) exercise elicit unique adaptations in skeletal muscle. The purpose here was to compare the post-exercise response of mTOR signaling and select autophagy markers in skeletal muscle to acute AE and RE.Methods In a randomized, cross-over design, six untrained men (27 ± 3 years) completed acute AE (40 min cycling, 70% HRmax) and RE (8 sets, 10 repetitions, 65% 1RM). Muscle biopsies were taken at baseline, and at 1 h and 4 h following each exercise. Western blot analyses were performed to examine total and phosphorylated protein levels. Upstream regulator analyses of skeletal muscle transcriptomics were performed to discern the predicted activation states of mTOR and FOXO3.Results Compared to AE, acute RE resulted in greater phosphorylation (P < 0.05) of mTOR Ser2448 at 4 h, S6K1 Thr389 at 1 h, and 4E- BP1 Thr37/46 during the post-exercise period. However, both AE and RE increased mTOR Ser2448 and S6K1 Thr389 phosphorylation at 4 h (P < 0.05). Upstream regulator analyses revealed the activation state of mTOR was increased for both AE (z score, 2.617) and RE (z score, 2.789). No changes in LC3BI protein were observed following AE or RE (P > 0.05), however, LC3BII protein was decreased after both AE and RE at 1 h and 4 h (P < 0.05). p62 protein content was also decreased at 4 h following AE and RE (P < 0.05).Conclusion Both acute AE and RE stimulate mTOR signaling and similarly impact select markers of autophagy. These findings indicate the early adaptive response of untrained human skeletal muscle to divergent exercise modes is not likely mediated through large differences in mTOR signaling or autophagy.
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- Authors
- Mazo, C. E., D’Lugos, A. C., Sweeney, K. R., Haus, J. M., Angadi, S. S., Carroll, C. C., & Dickinson, J. M.
- Affiliations
- University of Michigan (US)
- University of Florida Health (US)
- Arizona State University (US)
- University of Virginia (US)
- Purdue University West Lafayette (US)
- Central Washington University (US)
- Corresponding author
- Dickinson, Jared M.
- ORCID
- Venue
- European Journal of Applied Physiology
- Published
- 2021-07-01
- Volume
- 121
- Issue
- 10
- Pages
- 2913-2924
- ISSN
- 0020-9376 (ISSN-L)
- 1439-6319
- 1439-6327
- PMC
- PMC10150453
- OpenAlex
- W3175469519
- Cited by
- 36
- References
- 50
- Citations by year
- 2026: 5 · 2025: 11 · 2024: 9 · 2023: 6 · 2022: 5
- FWCI
- 2.61
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- European Journal of Applied Physiology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- ScholarWorks (Central Washington University) (submittedVersion)
- Funders
- Foundation for the National Institutes of Health (R01 DK109948, T32HL134621)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Keywords
- acute aerobic exercise, acute resistance exercise, mTOR signaling, autophagy, skeletal muscle, untrained men, exercise adaptation, LC3B, p62, FOXO3, exercise mode comparison
- MeSH terms
- Adaptation, Physiological, Adult, Autophagy, Humans, Male, Muscle Proteins, Exercise, Muscle, Skeletal, Resistance Training, TOR Serine-Threonine Kinases
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