Autophagy is not required to sustain exercise and PRKAA1/AMPK activity but is important to prevent mitochondrial damage during physical activity
Open accessAbstract
Physical activity has been recently documented to play a fundamental physiological role in the regulation of autophagy in several tissues. It has also been reported that autophagy is required for exercise itself and for training-induced adaptations in glucose homeostasis. These autophagy-mediated metabolic improvements are thought to be largely dependent on the activation of the metabolic sensor PRKAA1/AMPK. However, it is unknown whether these important benefits stem from systemic adaptations or are due solely to alterations in skeletal muscle metabolism. To address this we utilized inducible, muscle-specific, atg7 knockout mice that we have recently generated. Our findings indicate that acute inhibition of autophagy in skeletal muscle just prior to exercise does not have an impact on physical performance, PRKAA1 activation, or glucose homeostasis. However, we reveal that autophagy is critical for the preservation of mitochondrial function during damaging muscle contraction. This effect appears to be gender specific affecting primarily females. We also establish that basal oxidative stress plays a crucial role in mitochondrial maintenance during normal physical activity. Therefore, autophagy is an adaptive response to exercise that ensures effective mitochondrial quality control during damaging physical activity.
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Acute deletion of ATG7 specifically in skeletal muscle does not affect exercise performance, suggesting that autophagy is not required to sustain muscle contraction during exercise training.
“The acute deletion of ATG7, specifically in skeletal muscle, does not affect exercise performance suggesting that autophagy is not required to sustain muscle contraction during exercise training [222, 223].”
From The connection between the dynamic remodeling of the mitochondrial network and the regulation of muscle mass (2021) · cites this paper as [222]
Mitophagy activation removes dysfunctional mitochondria that have been altered by exercise-dependent ROS production.
“Moreover, mitophagy activation removes dysfunctional mitochondria that have been altered by exercise-dependent ROS production [222].”
From The connection between the dynamic remodeling of the mitochondrial network and the regulation of muscle mass (2021) · cites this paper as [222]
Activation of autophagy during exercise is important for maintaining muscle energy homeostasis.
“However, the activation of autophagy during exercise is important for maintaining muscle energy homeostasis.”
From The connection between the dynamic remodeling of the mitochondrial network and the regulation of muscle mass (2021) · cites this paper as [222]
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- Authors
- Lo Verso, F., Carnio, S., Vainshtein, A., & Sandri, M.
- Affiliations
- Veneto Institute of Molecular Medicine (IT)
- York University (CA)
- University of Padua (IT)
- Corresponding author
- Sandri, Marco
- Venue
- Autophagy
- Publisher
- Informa UK Limited
- Published
- 2014-10-30
- Volume
- 10
- Issue
- 11
- Pages
- 1883-1894
- ISSN
- 1554-8627 (ISSN-L)
- 1554-8635
- PMC
- PMC4502666
- OpenAlex
- W1994408958
- Cited by
- 146
- References
- 38
- Citations by year
- 2026: 5 · 2025: 5 · 2024: 12 · 2023: 13 · 2022: 6 · 2021: 21 · 2020: 11 · 2019: 12 · 2018: 20 · 2017: 13 · 2016: 16 · 2015: 12
- FWCI
- 10.32
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Autophagy (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Figshare (submittedVersion, cc-by)
- Padua Research Archive (University of Padova) (submittedVersion)
- Figshare (cc-by)
- Funders
- Fondation Leducq
- Fondazione Telethon
- Consiglio Nazionale delle Ricerche
- Topics
- Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Keywords
- physical activity, skeletal muscle, mitochondrial damage, mitochondrial quality control, oxidative stress, muscle contraction, glucose homeostasis, sex differences, physical performance
- MeSH terms
- Autophagy-Related Protein 7, Animals, Antioxidants, Autophagy, Female, Glucose, Homeostasis, Male, Microtubule-Associated Proteins, Mitochondria, Muscle Contraction, Physical Conditioning, Animal, Mice, Knockout, Oxidative Stress, Mice, AMP-Activated Protein Kinases
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