Enzymatic in situ saccharification of cellulose in aqueous-ionic liquid media
Abstract
The enzymatic saccharification of a model cellulosic substrate, Avicel PH-101, using an ionic liquid (IL), 1-ethyl-3-methylimidazolium diethylphosphate, was explored. After mixing the IL solution of cellulose with different volumes of 10 mM citrate buffer (pH 5.0), cellulase was directly added to the aqueous-IL mixture at 40 degrees C. When the volume of IL to water was greater than 3:2, little cellulase activity was observed. However, decreasing the volume ratio markedly enhanced enzymatic activity: an IL to water ratio of 1:4 (v/v) resulted in over 70% of the starting amount of cellulose (10 mg/ml) being converted to glucose and cellobiose.
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Hardwoods and softwoods may require prohibitive quantities of energy to grind to fine powders.
“In contrast, hardwoods and softwoods may require prohibitive quantities of energy to grind to fine powders.183,186”
From Deconstruction of lignocellulosic biomass with ionic liquids (2013) · cites this paper as [186]
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- Authors
- Kamiya, N., Matsushita, Y., Hanaki, M., Nakashima, K., Narita, M., Goto, M., & Takahashi, H.
- Affiliations
- Kyushu University (JP)
- Toyota Central Research and Development Laboratories (Japan) (JP)
- Corresponding author
- Kamiya, Noriho
- Venue
- Biotechnology Letters
- Published
- 2008-02-05
- Volume
- 30
- Issue
- 6
- Pages
- 1037-1040
- ISSN
- 0141-5492 (ISSN-L)
- 1573-6776
- OpenAlex
- W2043267151
- Cited by
- 207
- References
- 12
- Citations by year
- 2025: 3 · 2024: 3 · 2023: 4 · 2022: 6 · 2021: 6 · 2020: 8 · 2019: 8 · 2018: 10 · 2017: 17 · 2016: 22 · 2015: 14 · 2014: 13 · 2013: 20 · 2012: 21
- FWCI
- 11.51
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Biotechnology Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Physical Sciences › Chemical Engineering › Catalysis › Ionic liquids properties and applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
- Keywords
- enzymatic saccharification, cellulose, ionic liquids, cellulase, Avicel PH 101, glucose production, cellobiose, citrate buffer
- MeSH terms
- Cellobiose, Cellulase, Cellulose, Enzyme Activation, Glucose, Imidazoles, Ionic Liquids
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