Biodiesel production from heterotrophic microalgal oil
Abstract
The present study introduced an integrated method for the production of biodiesel from microalgal oil. Heterotrophic growth of Chlorella protothecoides resulted in the accumulation of high lipid content (55%) in cells. Large amount of microalgal oil was efficiently extracted from these heterotrophic cells by using n-hexane. Biodiesel comparable to conventional diesel was obtained from heterotrophic microalgal oil by acidic transesterification. The best process combination was 100% catalyst quantity (based on oil weight) with 56:1 molar ratio of methanol to oil at temperature of 30 degrees C, which reduced product specific gravity from an initial value of 0.912 to a final value of 0.8637 in about 4h of reaction time. The results suggested that the new process, which combined bioengineering and transesterification, was a feasible and effective method for the production of high quality biodiesel from microalgal oil.
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Cyanobacteria have received less attention than other feedstocks such as microalgae or crops for production of lipid-based biofuels.
“For the production of lipid-based biofuels, cyanobacteria have received less attention than other feedstocks such as microalgae (Miao and Wu 2006; Rodolfi et al. 2009) or crops.”
From Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering (2011) · cites this paper as Miao and Wu 2006
Information regarding the production of biodiesel from cyanobacteria or related parameters such as biochemical profile, growth rate and energy content of different species is scarce.
“but information regarding the production of biodiesel from these species or related parameters such as the biochemical profile, growth rate and energy content of the different species are scarce (Miao and Wu 2006).”
From Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering (2011) · cites this paper as Miao and Wu 2006
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- Authors
- Miao, X., & Wu, Q.
- Affiliations
- Ningde Normal University (CN)
- Tsinghua University (CN)
- Corresponding author
- Wu, Qingyu
- Venue
- Bioresource Technology
- Publisher
- Elsevier BV
- Published
- 2006-04
- Volume
- 97
- Issue
- 6
- Pages
- 841-846
- ISSN
- 0960-8524 (ISSN-L)
- OpenAlex
- W2151972396
- Cited by
- 1,358
- References
- 18
- Citations by year
- 2026: 14 · 2025: 19 · 2024: 31 · 2023: 47 · 2022: 60 · 2021: 62 · 2020: 53 · 2019: 81 · 2018: 76 · 2017: 84 · 2016: 77 · 2015: 112 · 2014: 130 · 2013: 126 · 2012: 112
- FWCI
- 11.80
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bioresource Technology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China
- Ministry of Science and Technology of the People's Republic of China
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Engineering › Biomedical Engineering › Biodiesel Production and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- biodiesel production, heterotrophic microalgae, Chlorella protothecoides, microalgal lipid accumulation, hexane extraction, transesterification, acid catalysts, methanol to oil molar ratio, biodiesel properties
- MeSH terms
- Methanol, Biotechnology, Catalysis, Chlorella, Gasoline, Oils, Temperature
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