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IL-17 Exerts Anti-Apoptotic Effect via miR-155-5p Downregulation in Experimental Autoimmune Encephalomyelitis

Ksiazek-Winiarek, Dominika, Szpakowski, Piotr, Turniak, Malgorzata, Szemraj, Janusz, Glabinski, Andrzej·2017·Journal of Molecular Neuroscience
DOI 10.1007/s12031-017-0981-2PMC PMC5696500
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Fig. 1 The mice were used in two independent experiments. In the first, one healthy animal and immunized mice were subjected to stereotactic brain microinjection of IL-17 or PBS (phosphate-buffered saline) (n = 10 per group). In the second experiment, immunized mice were sacrificed in different stages of disease (n = 10 per group)
Fig. 1 The mice were used in two independent experiments. In the first, one healthy animal and immunized mice were subjected to stereotactic brain microinjection of IL-17 or PBS (phosphate-buffered saline) (n = 10 per group). In the second experiment, immunized mice were sacrificed in different stages of disease (n = 10 per group)CC BY · PubMed Central
Fig. 2 Scatterplot of relative expression of 84 microRNAs from Mouse Inflammatory Response and Autoimmunity microRNA PCR array, analyzed in brain homogenates of mice in preclinical phase of EAE, after stereotactic microinjections of IL-17 or PBS (control) (see the “Materials and Methods” section). Gray lines in the scatterplot indicate + 2.0 and – 2.0 fold change, which were used as cutoff values for selection of candidate miRNAs (black circles represent upregulated microRNAs, black triangles represent downregulated microRNAs)
Fig. 2 Scatterplot of relative expression of 84 microRNAs from Mouse Inflammatory Response and Autoimmunity microRNA PCR array, analyzed in brain homogenates of mice in preclinical phase of EAE, after stereotactic microinjections of IL-17 or PBS (control) (see the “Materials and Methods” section). Gray lines in the scatterplot indicate + 2.0 and – 2.0 fold change, which were used as cutoff values for selection of candidate miRNAs (black circles represent upregulated microRNAs, black triangles represent downregulated microRNAs)CC BY · PubMed Central
Fig. 3 Relative expression level of microRNAs during the course of EAE. RNA isolated from the brain of mice at various clinical stages were used. Results from all disease phases (preclinical, relapse, remission) were refered to results obtained from healthy mice (control) and presented as a fold change (2−∆∆CT)
Fig. 3 Relative expression level of microRNAs during the course of EAE. RNA isolated from the brain of mice at various clinical stages were used. Results from all disease phases (preclinical, relapse, remission) were refered to results obtained from healthy mice (control) and presented as a fold change (2−∆∆CT)CC BY · PubMed Central
Fig. 4 microRNA expression level after stereotactic brain microinjections of IL-17 in healthy mice and animals in preclinical phase of EAE. RNAs isolated from brain homogenates were used (see the “Materials and Methods” section). Results obtained for animals after intracerebral injection of IL-17 were referred to the results from animals after PBS microinjections (control), and presented as a fold change (2−∆∆CT)
Fig. 4 microRNA expression level after stereotactic brain microinjections of IL-17 in healthy mice and animals in preclinical phase of EAE. RNAs isolated from brain homogenates were used (see the “Materials and Methods” section). Results obtained for animals after intracerebral injection of IL-17 were referred to the results from animals after PBS microinjections (control), and presented as a fold change (2−∆∆CT)CC BY · PubMed Central
Fig. 5 Computational analysis of miR-155-5p target mRNAs and altered pathway—neurotrophin signaling pathway. Targets analyzed in our study are marked in gray squares (graph based on the KEGG pathway mmu04722 (Kanehisa and Goto 2000; Kanehisa et al. 2016))
Fig. 5 Computational analysis of miR-155-5p target mRNAs and altered pathway—neurotrophin signaling pathway. Targets analyzed in our study are marked in gray squares (graph based on the KEGG pathway mmu04722 (Kanehisa and Goto 2000; Kanehisa et al. 2016))CC BY · PubMed Central
Fig. 6 mRNA expression level after stereotactic brain microinjections of IL-17 to mice in preclinical phase of EAE. RNAs isolated from brain homogenates were used (see the “Materials and Methods” section). Results obtained for animals after intracerebral injection of IL-17 were referred to the results obtained for animals after PBS microinjections (control), and presented as a fold change (2−∆∆CT) ***p < 0.001
Fig. 6 mRNA expression level after stereotactic brain microinjections of IL-17 to mice in preclinical phase of EAE. RNAs isolated from brain homogenates were used (see the “Materials and Methods” section). Results obtained for animals after intracerebral injection of IL-17 were referred to the results obtained for animals after PBS microinjections (control), and presented as a fold change (2−∆∆CT) ***p < 0.001CC BY · PubMed Central
Fig. 7 Analysis of correlation between miR-155-5p and mRNA for: BDNF (a), HRAS (b), and BCL2 (c). Analysis was performed for results obtained from mice in preclinical phase of disease, after intracerebral injections of IL-17 or PBS (control). r Pearson’s correlation coefficient, R Spearman’s correlation coefficient
Fig. 7 Analysis of correlation between miR-155-5p and mRNA for: BDNF (a), HRAS (b), and BCL2 (c). Analysis was performed for results obtained from mice in preclinical phase of disease, after intracerebral injections of IL-17 or PBS (control). r Pearson’s correlation coefficient, R Spearman’s correlation coefficientCC BY · PubMed Central
Fig. 8 The protein level studied in the homogenates of brains from preclinical EAE mice after intracerebral injection of IL-17 or PBS (control)—BDNF (a); HRas (b); Bcl-2 (c) expression. Data are presented as mean ± SD; *p < 0.05
Fig. 8 The protein level studied in the homogenates of brains from preclinical EAE mice after intracerebral injection of IL-17 or PBS (control)—BDNF (a); HRas (b); Bcl-2 (c) expression. Data are presented as mean ± SD; *p < 0.05CC BY · PubMed Central

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Metadata

Authors
Ksiazek-Winiarek, D., Szpakowski, P., Turniak, M., Szemraj, J., & Glabinski, A.
Venue
Journal of Molecular Neuroscience
Publisher
Springer Science and Business Media LLC
Published
2017-10-23
Volume
63
Issue
3-4
Pages
320-332
ISSN
  • 0895-8696
  • 1559-1166
DOI
10.1007/s12031-017-0981-2
PMC
PMC5696500
Cited by
26
References
4
Language
en
Licence
creativecommons.org/licenses/by/4.0 (unspecified)
Funders
  • Narodowe Centrum Nauki (DEC-2012/05/N/NZ4/02347)
  • Uniwersytet Medyczny w Lodzi (503/5-062-01/503-51-002)

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