Vicia villosa lectin-positive neurones in human cerebral cortex. Loss in Alzheimer-type dementia
Abstract
Frontal and temporal sections of 4 normal human brains and 3 brains from patients with a diagnosis of Alzheimer-type dementia (ATD) were stained with Vicia villosa lectin conjugated to biotin to localize glycoproteins containing terminal N-acetylgalactosamine. These lectin binding sites were localized at the surface of a population of non-pyramidal interneurones. Examination of the co-existence of calbindin D28K or parvalbumin (PVA) with the lectin-binding material revealed that in the human cerebral cortex the lectin labelled only the PVA-immunoreactive neurones. Cell counts of the number of lectin-staining cells revealed that these cells are lost in ATD. The few remaining lectin-staining cells in ATD are shrunken and showed reduced expression of the lectin-staining material.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Studies using VVA and WFA lectin staining of PNN CS-GAGs showed up to 70% reductions in abundance of PNN CS glycan attachments in postmortem AD brain tissue.
“Studies using Vicia villosa agglutinin (VVA) and Wisteria floribunda agglutinin (WFA) lectin staining of PNN CS-GAGs showed up to 70% reductions in the abundance of PNN CS glycan attachments in postmortem AD brain tissue (Kobayashi et al. 1989; Baig et al. 2005).”
From Perineuronal net deglycosylation associates with tauopathy‐induced gliosis and neurodegeneration (2024) · cites this paper as Kobayashi et al. 1989
Cites
Amyloid β Protein Gene: cDNA, mRNA Distribution, and Genetic Linkage Near the Alzheimer Locus
Tanzi, R. E., Gusella, J. F., Watkins, P. C., Bruns, G. A. P., St George-Hyslop, P., Van Keuren, M. L., Patterson, D., Pagan, S., Kurnit, D. M., & Neve, R. L. (1987) · Science · doi:10.1126/science.2949367 · Cited by 1,611 · see citing works
Ubiquitin Is a Component of Paired Helical Filaments in Alzheimer's Disease
Mori, H., Kondo, J., & Ihara, Y. (1987) · Science · doi:10.1126/science.3029875 · Cited by 843 · see citing works
Some morphometric aspects of the brain in senile dementia of the alzheimer type
Terry, R. D., Peck, A., DeTeresa, R., Schechter, R., & Horoupian, D. S. (1981) · Annals of Neurology · doi:10.1002/ana.410100209 · Cited by 801 · see citing works
Parvalbumin in Most γ-Aminobutyric Acid-Containing Neurons of the Rat Cerebral Cortex
Celio, M. R. (1986) · Science · doi:10.1126/science.3945815 · Cited by 740 · see citing works
Bird, E. D., & Iversen, L. L. (1974) · Brain · doi:10.1093/brain/97.1.457 · Cited by 529 · see citing works
Conservation of brain amyloid proteins in aged mammals and humans with Alzheimer's disease.
Selkoe, D. J., Bell, D., Podlisny, M., Price, D. L., & Cork, L. (1987) · Science · doi:10.1126/science.3544219 · Cited by 471 · see citing works
Cortical neuronal counts in normal elderly controls and demented patients
Mountjoy, C. Q., Roth, M., Evans, N. J. R., & Evans, H. M. (1983) · Neurobiology of Aging · doi:10.1016/0197-4580(83)90048-9 · Cited by 291 · see citing works
Self-citationKosaka, T., & Heizmann, C. W. (1989) · Brain Research · doi:10.1016/0006-8993(89)90048-6 · Cited by 153 · see citing works
Nakagawa, F., Schulte, B. A., & Spicer, S. S. (1986) · The Journal of Comparative Neurology · doi:10.1002/cne.902430210 · Cited by 140 · see citing works
Loss of parvalbumin-immunoreactive neurones from cortex in Alzheimer-type dementia
Arai, H., Emson, P. C., Mountjoy, C. Q., Carassco, L. H., & Heizmann, C. W. (1987) · Brain Research · doi:10.1016/0006-8993(87)90974-7 · Cited by 118 · see citing works
Self-citationLoss of calbindin-28K immunoreactive neurones from the cortex in Alzheimer-type dementia
Ichimiya, Y., Emson, P. C., Mountjoy, C. Q., Lawson, D. E. M., & Heizmann, C. W. (1988) · Brain Research · doi:10.1016/0006-8993(88)90210-7 · Cited by 115 · see citing works
Self-citationMolecular determinants of GABAergic local-circuit neurons in the visual cortex
Naegele, J. R., & Barnstable, C. J. (1989) · Trends in Neurosciences · doi:10.1016/0166-2236(89)90153-7 · Cited by 100 · see citing works
Nakagawa, F., Schulte, B. A., Wu, J. Y., & Spicer, S. S. (1986) · Journal of Neurocytology · doi:10.1007/bf01611440 · Cited by 67 · see citing works
Cerebral cortical calbindin D28K and parvalbumin neurones in Down's syndrome
Kobayashi, K., Emson, P. C., Mountjoy, C. Q., Thornton, S. N., Lawson, D. E. M., & Mann, D. M. A. (1990) · Neuroscience Letters · doi:10.1016/0304-3940(90)90487-t · Cited by 57 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Calcium-binding proteins: selective markers of nerve cells
Andressen, C., Bl�mcke, I., & Celio, M. R. (1993) · Cell and Tissue Research · doi:10.1007/bf00318606 · Cited by 701 · see citing works
Galtrey, C. M., & Fawcett, J. W. (2007) · Brain Research Reviews · doi:10.1016/j.brainresrev.2006.09.006 · Cited by 575 · see citing works
DeFelipe, J. (1997) · Journal of Chemical Neuroanatomy · doi:10.1016/s0891-0618(97)10013-8 · Cited by 550 · see citing works
Perineuronal nets: past and present
Celio, M. R., Spreafico, R., De Biasi, S., & Vitellaro-Zuccarello, L. (1998) · Trends in Neurosciences · doi:10.1016/s0166-2236(98)01298-3 · Cited by 497 · see citing works
GABAergic Neurons and Their Role in Cortical Plasticity in Primates
Jones, E. G. (1993) · Cerebral Cortex · doi:10.1093/cercor/3.5.361-a · Cited by 489 · see citing works
Perineuronal nets — a specialized form of extracellular matrix in the adult nervous system
Celio, M. R., & Blümcke, I. (1994) · Brain Research Reviews · doi:10.1016/0165-0173(94)90006-x · Cited by 386 · see citing works
Microglia facilitate loss of perineuronal nets in the Alzheimer's disease brain
Crapser, J. D., Spangenberg, E. E., Barahona, R. A., Arreola, M. A., Hohsfield, L. A., & Green, K. N. (2020) · EBioMedicine · doi:10.1016/j.ebiom.2020.102919 · Cited by 280 · see citing works
Targeting the neural extracellular matrix in neurological disorders
Soleman, S., Filippov, M. A., Dityatev, A., & Fawcett, J. W. (2013) · Neuroscience · doi:10.1016/j.neuroscience.2013.08.050 · Cited by 244 · see citing works
Brückner, G., Hausen, D., Härtig, W., Drlicek, M., Arendt, T., & Brauer, K. (1999) · Neuroscience · doi:10.1016/s0306-4522(99)00071-8 · Cited by 196 · see citing works
Microglia as hackers of the matrix: sculpting synapses and the extracellular space
Crapser, J. D., Arreola, M. A., Tsourmas, K. I., & Green, K. N. (2021) · Cellular & Molecular Immunology · doi:10.1038/s41423-021-00751-3 · Cited by 178 · see citing works
Morawski, M., Brückner, G., Jäger, C., Seeger, G., Matthews, R. T., & Arendt, T. (2012) · Brain Pathology · doi:10.1111/j.1750-3639.2011.00557.x · Cited by 157 · see citing works
An Extracellular Perspective on CNS Maturation: Perineuronal Nets and the Control of Plasticity
Carulli, D., & Verhaagen, J. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22052434 · Cited by 123 · see citing works
Ariza, J., Rogers, H., Hashemi, E., Noctor, S. C., & Martínez-Cerdeño, V. (2018) · Cerebral Cortex · doi:10.1093/cercor/bhw349 · Cited by 101 · see citing works
Extracellular matrix proteomics in schizophrenia and Alzheimer’s disease
Sethi, M. K., & Zaia, J. (2016) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-016-9900-6 · Cited by 109 · see citing works
Loss of perineuronal net N-acetylgalactosamine in Alzheimer’s disease
Baig, S., Wilcock, G. K., & Love, S. (2005) · Acta Neuropathologica · doi:10.1007/s00401-005-1060-2 · Cited by 112 · see citing works
Morris, N. P., & Henderson, Z. (2000) · European Journal of Neuroscience · doi:10.1046/j.1460-9568.2000.00970.x · Cited by 98 · see citing works
Perineuronal nets and schizophrenia: The importance of neuronal coatings
Bitanihirwe, B. K. Y., & Woo, T.-U. W. (2014) · Neuroscience & Biobehavioral Reviews · doi:10.1016/j.neubiorev.2014.03.018 · Cited by 95 · see citing works
Lendvai, D., Morawski, M., Négyessy, L., Gáti, G., Jäger, C., Baksa, G., Glasz, T., Attems, J., Tanila, H., Arendt, T., et al. (2013) · Acta Neuropathologica · doi:10.1007/s00401-012-1042-0 · Cited by 93 · see citing works
Cattaud, V., Bezzina, C., Rey, C. C., Lejards, C., Dahan, L., & Verret, L. (2018) · Neurobiology of Aging · doi:10.1016/j.neurobiolaging.2018.08.024 · Cited by 88 · see citing works
Phillips, R. G., Meier, T. J., Giuli, L. C., McLaughlin, J. R., Ho, D. Y., & Spolsky, R. M. (1999) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1999.0731200.x · Cited by 87 · see citing works
ECM in brain aging and dementia
Morawski, M., Filippov, M., Tzinia, A., Tsilibary, E., & Vargova, L. (2014) · Progress in Brain Research · doi:10.1016/b978-0-444-63486-3.00010-4 · Cited by 84 · see citing works
Angelov, D. N., Walther, M. R., Streppel, M., Guntinas‐Lichius, O., Neiss, W. F., Probstmeier, R., & Pesheva, P. (1998) · Journal of Neuroscience · doi:10.1523/jneurosci.18-16-06218.1998 · Cited by 77 · see citing works
Yang, S., Hilton, S., Alves, J. N., Saksida, L. M., Bussey, T., Matthews, R. T., Kitagawa, H., Spillantini, M. G., Kwok, J. C. F., & Fawcett, J. W. (2017) · Neurobiology of Aging · doi:10.1016/j.neurobiolaging.2017.08.002 · Cited by 61 · see citing works
The “Loss” of Perineuronal Nets in Alzheimer's Disease: Missing or Hiding in Plain Sight?
Scarlett, J. M., Hu, S. J., & Alonge, K. M. (2022) · Frontiers in Integrative Neuroscience · doi:10.3389/fnint.2022.896400 · Cited by 61 · see citing works
Shapiro, L. S., Halpern, M., & Ee, P. (1995) · Journal of Morphology · doi:10.1002/jmor.1052240307 · Cited by 59 · see citing works
Decoding perineuronal net glycan sulfation patterns in the Alzheimer's disease brain
Logsdon, A. F., Francis, K. L., Richardson, N. E., HU, S. J. W., Faber, C. L., Phan, B. A., Nguyen, V., Setthavongsack, N., Banks, W. A., Woltjer, R. L., et al. (2021) · Alzheimer s & Dementia · doi:10.1002/alz.12451 · Cited by 55 · see citing works
Perineuronal and perisynaptic extracellular matrix in the human spinal cord
Jäger, C., Lendvai, D., Seeger, G., Brückner, G., Matthews, R. T., Arendt, T., Alpár, A., & Morawski, M. (2013) · Neuroscience · doi:10.1016/j.neuroscience.2013.02.014 · Cited by 48 · see citing works
Schüppel, K., Brauer, K., Härtig, W., Grosche, J., Earley, B., Leonard, B. E., & Brückner, G. (2002) · Brain Research · doi:10.1016/s0006-8993(02)03569-2 · Cited by 47 · see citing works
Härtig, W. (2001) · Neurobiology of Aging · doi:10.1016/s0197-4580(00)00179-2 · Cited by 46 · see citing works
Neural ECM in regeneration and rehabilitation
Yang, S., Kwok, J. C. F., & Fawcett, J. W. (2014) · Progress in brain research · doi:10.1016/b978-0-444-63486-3.00008-6 · Cited by 37 · see citing works
Santos‐Silva, T., Colodete, D. A. E., Lisboa, J. R., Freitas, I. D. S., Lopes, C. F. B., Hadera, V., Lima, T. S. A., Souza, A. J., & Gomes, F. V. (2024) · Basic & Clinical Pharmacology & Toxicology · doi:10.1111/bcpt.13994 · Cited by 36 · see citing works
Regulation of the E/I-balance by the neural matrisome
Mueller-Buehl, C., Wegrzyn, D., Bauch, J., & Faissner, A. (2023) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2023.1102334 · Cited by 32 · see citing works
GABA Neurons and Their Role in Activity-Dependent Plasticity of Adult Primate Visual Cortex
Jones, E. G., Hendry, S. H. C., DeFelipe, J., & Benson, D. L. (1994) · Cerebral cortex · doi:10.1007/978-1-4757-9628-5_3 · Cited by 32 · see citing works
GABAergic complex basket formations in the human neocortex
Blázquez‐Llorca, L., García‐Marín, V., & DeFelipe, J. (2010) · The Journal of Comparative Neurology · doi:10.1002/cne.22496 · Cited by 30 · see citing works
Li, X., Wu, X., Lu, T., Kuang, C., Si, Y., Zheng, W., Li, Z., & Xue, Y.-X. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25063412 · Cited by 25 · see citing works
Organization of neural inputs to the suprachiasmatic nucleus in the rat
Moga, M. M., & Moore, R. Y. (1997) · The Journal of Comparative Neurology · doi:10.1002/(sici)1096-9861(19971222)389:3 · Cited by 308 · see citing works
Lectin histochemistry in the aged dog brain
Kiatipattanasakul, W., Nakayama, H., Nakamura, S., & Doi, K. (1998) · Acta Neuropathologica · doi:10.1007/s004010050796 · Cited by 17 · see citing works
Perineuronal net deglycosylation associates with tauopathy‐induced gliosis and neurodegeneration
Logsdon, A. F., Foresi, B., Hu, S. J., Quah, E., Meuret, C. J., Le, J. P., Hendrickson, A. S., Redford, I. K., Kumar, A., Phan, B. A., et al. (2024) · Journal of Neurochemistry · doi:10.1111/jnc.16067 · Cited by 16 · see citing works
Zhang, W., Liu, T., Li, J., Singh, J., Chan, A., Islam, A., Petrache, A. L., Peng, Y., Harvey, K., & Ali, A. B. (2024) · British Journal of Pharmacology · doi:10.1111/bph.16441 · Cited by 14 · see citing works
Rahmani, R., Rambarack, N., Singh, J., Constanti, A., & Ali, A. B. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms241914917 · Cited by 11 · see citing works
A review of Vicieae lectins studies: End of the book or a story in the writing?
Cavada, B. S., Pinto‐Junior, V. R., Oliveira, M. V., Osterne, V. J. S., Lóssio, C. F., & Nascimento, K. S. (2021) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2021.04.062 · Cited by 7 · see citing works
Hendrickson, A. S., Francis, K. L., Kumar, A., Le, J. P., Scarlett, J. M., Keene, C. D., Tovar, D. A., & Alonge, K. M. (2024) · Frontiers in Neuroscience · doi:10.3389/fnins.2024.1396101 · Cited by 6 · see citing works
Perineuronal Nets: Old Structures, New Functions
Tewari, B. P., Chaunsali, L., & Sontheimer, H. W. (2026) · The Neuroscientist · doi:10.1177/10738584261421180 · Cited by 3 · see citing works
Huang, Y.-F., Liu, C., Liu, Z., Zhuang, X., Chen, J.-H., Zhang, Z.-N., Shen, C., & Hu, Y. (2026) · Brain · doi:10.1093/brain/awag058 · Cited by 2 · see citing works
Terstege, D. J., & Epp, J. R. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.01.15.633249 · Cited by 2 · see citing works
Role of extracellular space in neurodegenerative diseases
Estaún‐Panzano, J. (2024) · doi:10.70675/98d1d96eza025z406ezba93z33e0820c3cf3
- Authors
- Kobayashi, K., Emson, P. C., & Mountjoy, C. Q.
- Affiliations
- University of Cambridge (GB)
- Corresponding author
- Emson, P.C.
- ORCID
- Kobayashi, K.
- Venue
- Brain Research
- Publisher
- Elsevier BV
- Published
- 1989-09
- Volume
- 498
- Issue
- 1
- Pages
- 170-174
- ISSN
- 0006-8993 (ISSN-L)
- OpenAlex
- W2092639383
- Cited by
- 48
- References
- 14
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 6 · 2023: 2 · 2022: 1 · 2021: 4 · 2020: 2 · 2018: 2 · 2017: 1 · 2016: 2 · 2014: 3 · 2013: 2 · 2012: 1
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
- Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
- Keywords
- Vicia villosa lectin, N-acetylgalactosamine, parvalbumin, parvalbumin interneurons, Alzheimer-type dementia, cerebral cortex, lectin binding sites, calbindin-D28k, neuronal loss, glycoproteins
- MeSH terms
- Acetylgalactosamine, Aged, Aged, 80 and over, Alzheimer Disease, Cell Count, Cerebral Cortex, Galactosamine, Glycoproteins, Humans, Lectins, Plant Lectins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
