Cortical cholinergic function and deficits in visual attentional performance in rats following 192 IgG–saporin-induced lesions of the medial prefrontal cortex
Abstract
Lesions of the basal forebrain (BF) cortical cholinergic system impair performance on a rodent five-choice visual attentional task. This study examines the effects on the same task of selective depletion of acetylcholine from the prefrontal cortex (PFC) using 192 IgG-saporin, the cholinergic immunotoxin. Rats were trained to detect brief visual stimuli, either presented unpredictably both temporally and spatially to increase attentional load, or under less demanding conditions where stimuli were temporally and spatially predictable. Following training, 192 IgG-saporin (50 ng or 100 ng/infusion) or its vehicle was infused bilaterally into the ventromedial PFC. The 100 ng lesion group exhibited post-operatively a transient increase in perseveration, specifically when the visual stimuli were temporally unpredictable. A vigilance decrement, as well as a reinstatement of perseverative responding occurred in both lesion groups under conditions of enhanced attentional load, specifically with high target frequency sustained over many trials. Lesioned subjects were also more impulsive with increased anticipatory errors. Systemic administration of the muscarinic receptor antagonist scopolamine further dissociated the groups with attentional accuracy in the 100 ng group decreasing relative to shams. These findings are consistent with an important modulatory influence of PFC function by BF cholinergic neurons, particularly during increased attentional demand.
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Lesions of the anterior cingulate cortex disrupt performance on the 5-Choice Serial Reaction Time Task (5-CSRTT).
“Lesions of the anterior cingulate cortex disrupt performance on the 5-Choice Serial Reaction Time Task (5-CSRTT) (Dalley et al., 2004)”
From The cingulum bundle: Anatomy, function, and dysfunction (2018) · cites this paper as Dalley et al., 2004
The Introduction (page unavailable) reports that lesions of the entire medial prefrontal cortex disrupt 5-CSRT task performance, but it does not establish that lesions specifically of the anterior cingulate cortex do so. It also reports performance changes after lesions that spare the anterior cingulate cortex. The paper’s own results concern cholinergic lesions of the medial prefrontal cortex, not isolated anterior cingulate lesions. (LLM, conf=0.86)
Experimental — accuracy not yet measuredCholinergic activity is intrinsic to 5-CSRTT performance.
“while related studies show how cholinergic activity is intrinsic to 5-CSRTT performance (Dalley et al., 2004).”
From The cingulum bundle: Anatomy, function, and dysfunction (2018) · cites this paper as Dalley et al., 2004
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- Authors
- Dalley, J. W.
- Affiliations
- University of Cambridge (GB)
- Corresponding author
- Dalley, J. W.
- ORCID
- Dalley, J. W.
- Venue
- Cerebral Cortex
- Publisher
- Oxford University Press (OUP)
- Published
- 2004-03-28
- Volume
- 14
- Issue
- 8
- Pages
- 922-932
- ISSN
- 1047-3211 (ISSN-L)
- 1460-2199
- OpenAlex
- W2171934036
- Cited by
- 191
- References
- 3
- Citations by year
- 2026: 4 · 2025: 6 · 2024: 9 · 2023: 3 · 2022: 7 · 2021: 6 · 2020: 6 · 2019: 8 · 2018: 11 · 2017: 7 · 2016: 10 · 2015: 9 · 2014: 13 · 2013: 10 · 2012: 10
- FWCI
- 4.34
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cerebral Cortex (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
- Life Sciences › Neuroscience › Cognitive Neuroscience › Neural and Behavioral Psychology Studies
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- medial prefrontal cortex, 192 IgG-saporin, acetylcholine depletion, visual attention, 5-choice serial reaction time task, perseveration, vigilance decrement, impulsivity, scopolamine, muscarinic receptors, basal forebrain cholinergic neurons, attentional load, rats
- MeSH terms
- Saporins, Acetylcholine, Animals, Antibodies, Monoclonal, Immunotoxins, Arousal, Attention Deficit Disorder with Hyperactivity, Male, N-Glycosyl Hydrolases, Tissue Distribution, Vision Disorders, Prefrontal Cortex, Rats, Ribosome Inactivating Proteins, Type 1
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