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Sorting mechanisms of Tau and MAP2 in neurons: Suppressed axonal transit of MAP2 and locally regulated microtubule binding
Open accessAbstract
Tau is abundant in the axon, whereas MAP2 is found in the cell body and dendrites. To understand their differential localization, we performed transfection studies on primary cultured neurons using tagged tau, MAP2, MAP2C, and their chimeric/deletion mutants. We found that MAP2 was prevented from entering the axon by its N-terminal projection domain and that microtubule binding of tau was stronger in the axon than in the cell body and dendrites, whereas that of MAP2/MAP2C was tighter in the cell body and dendrites than in the axon. These binding properties were determined by their microtubule-binding domains and were suggested to be regulated by phosphorylation, at least in the case of tau. Thus, the suppressed axonal transit of MAP2 and locally regulated microtubule binding may play important roles for their sorting in neurons.
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“Kanai et al. (6) also reported that the projection domain (PD) of MAP2, which exists only in MAP2A and B, prevents MAP2 from entering the axon, resulting in the somatodendritic localization of HMW MAP2”
From Active transport by cytoplasmic dynein sustains dendritic MAP2 localization in developing neurons (2026) · cites this paper as [6]
In the citing paper
“Tau is highly expressed in neurons in the mammalian brain, and normally localizes predominantly to axons as an important regulator of axonal transport [124–126].”
From Molecular and cellular mechanisms underlying the pathogenesis of Alzheimer’s disease (2020) · cites this paper as [125]
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Metadata
- Authors
- Kanai, Y., & Hirokawa, N.
- Affiliations
- The University of Tokyo (JP)
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 1995-02
- Volume
- 14
- Issue
- 2
- Pages
- 421-432
- ISSN
- 0896-6273 (ISSN-L)
- OpenAlex
- W2039754449
- Cited by
- 144
- References
- 70
- Citations by year
- 2026: 4 · 2025: 2 · 2024: 3 · 2023: 7 · 2022: 2 · 2021: 4 · 2020: 5 · 2019: 7 · 2018: 4 · 2017: 5 · 2016: 4 · 2015: 7 · 2014: 6 · 2013: 4 · 2012: 6
- FWCI
- 5.86
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Neuron (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Alzheimer's disease research and treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- tau protein, MAP2, MAP2c, axonal transport, microtubule binding, projection domain, tau phosphorylation, neuronal cytoskeleton, chimeric mutants
- MeSH terms
- Amino Acid Sequence, Animals, Axons, Cells, Cultured, Dendrites, Embryo, Mammalian, Fluorescent Antibody Technique, Microtubule-Associated Proteins, Microtubules, Molecular Sequence Data, Neurons, Protein Binding, Sheep, Spinal Cord, Transfection, Proto-Oncogene Proteins c-myc, tau Proteins, Sequence Homology, Amino Acid, In Situ Hybridization, Mice
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