Involvement of hippocampal NMDA receptors in retrieval of spontaneous object recognition memory in rats
Abstract
The involvement of hippocampal N-methyl-d-aspartate (NMDA) receptors in the retrieval process of spontaneous object recognition memory was investigated. The spontaneous object recognition test consisted of three phases. In the sample phase, rats were exposed to two identical objects several (2-5) times in the arena. After the sample phase, various lengths of delay intervals (24h-6 weeks) were inserted (delay phase). In the test phase in which both the familiar and the novel objects were placed in the arena, rats' novel object exploration behavior under the hippocampal treatment of NMDA receptor antagonist, AP5, or vehicle was observed. With 5 exposure sessions in the sample phase (experiment 1), AP5 treatment in the test phase significantly decreased discrimination ratio when the delay was 3 weeks but not when it was one week. On the other hand, with 2 exposure sessions in the sample phase (experiment 2) in which even vehicle-injected control animals could not discriminate the novel object from the familiar one with a 3 week delay, AP5 treatment significantly decreased discrimination ratio when the delay was one week, but not when it was 24h. Additional experiment (experiment 3) showed that the hippocampal treatment of an α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor antagonist, NBQX, decreased discrimination ratio with all delay intervals tested (24h-3 weeks). Results suggest that hippocampal NMDA receptors play an important role in the retrieval of spontaneous object recognition memory especially when the memory trace weakens.
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Infusion of NBQX, a potent AMPA-R antagonist, impaired memory irrespective of the delays.
“infusion of 2,3-Dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide (NBQX), a potent AMPA-R antagonist, impaired the memory irrespective of the delays”
From The medial prefrontal cortex - hippocampus circuit that integrates information of object, place and time to construct episodic memory in rodents: Behavioral, anatomical and neurochemical properties (2020) · cites this paper as Iwamura et al., 2016
Pre-test infusions of AP5 into the hippocampus disrupted NOP performance only when the memory was weak.
“pre-test infusions of AP5 into the hippocampus disrupted NOP performance only when the memory was weak”
From The medial prefrontal cortex - hippocampus circuit that integrates information of object, place and time to construct episodic memory in rodents: Behavioral, anatomical and neurochemical properties (2020) · cites this paper as Iwamura et al., 2016
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- Authors
- Iwamura, E., Yamada, K., & Ichitani, Y.
- Affiliations
- University of Tsukuba (JP)
- Corresponding author
- Ichitani, Yukio
- ORCID
- Yamada, Kazuo
- Venue
- Behavioural Brain Research
- Publisher
- Elsevier BV
- Published
- 2016-07
- Volume
- 307
- Pages
- 92-99
- ISSN
- 0166-4328 (ISSN-L)
- OpenAlex
- W2320297780
- Cited by
- 20
- References
- 29
- Citations by year
- 2025: 3 · 2024: 1 · 2023: 3 · 2022: 3 · 2021: 2 · 2020: 2 · 2019: 4 · 2018: 1 · 2017: 1
- FWCI
- 1.28
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Behavioural Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science (26590174, 24530909)
- Topics
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory Processes and Influences
- Keywords
- memory retrieval, AP5, NBQX, AMPA receptor antagonism, retention interval, novel object discrimination, rats
- MeSH terms
- Analysis of Variance, Animals, Discrimination Learning, Hippocampus, Male, Quinoxalines, Mental Recall, Time Factors, Valine, Receptors, N-Methyl-D-Aspartate, Rats, Wistar, Excitatory Amino Acid Agents, Recognition, Psychology, Rats
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