Production of biomass and nutraceutical compounds by Spirulina platensis under different temperature and nitrogen regimes
Abstract
The cyanobacterium Spirulina platensis has been used by humans because of its nutritional and possibly medicinal effects. Our study evaluated the influence of temperature and nitrogen concentration in the medium on the production of biomass by this cyanobacterium and the biomass composition in protein, lipid and phenolic compounds. We found that at 35 degrees C there was a negative effect on biomass production but a positive effect on the production of protein, lipids and phenolics, the highest levels of these compounds being obtained in Zarrouk's medium containing 1.875 or 2.500 g l(-1) sodium nitrate. Higher biomass densities and productivity were obtained at 30 degrees C than at 35 degrees C, but nitrogen concentration appeared to have no effect on the amount of protein, lipid or phenolics, indicating that at 30 degrees C the concentration of sodium nitrate in Zarrouk's medium (2.50 g l(-1)) can be reduced without loss of productivity, an important cost-saving factor in large-scale cultivation.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Spirulina has gained worldwide popularity as a food supplement.
“and has gained worldwide popularity as a food supplement (Colla et al., 2007).”
From Microalgae Metabolites: A Rich Source for Food and Medicine (2019) · cites this paper as Colla et al., 2007
Cites
A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189 · see citing works
Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents
Singleton, V. L., & Rossi, J. A. (1965) · American Journal of Enology and Viticulture · doi:10.5344/ajev.1965.16.3.144 · Cited by 24,852 · see citing works
Spirulina Platensis Arthrospira
(1997) · doi:10.1201/9781482272970 · Cited by 704 · see citing works
Anupama, & Ravindra, P. (2000) · Biotechnology Advances · doi:10.1016/s0734-9750(00)00045-8 · Cited by 632 · see citing works
Piorreck, M., Baasch, K. L.-K., & Pohl, P. (1984) · Phytochemistry · doi:10.1016/s0031-9422(00)80304-0 · Cited by 538 · see citing works
Current knowledge on potential health benefits of Spirulina
Belay, A., Ota, Y., Miyakawa, K., & Shimamatsu, H. (1993) · Journal of Applied Phycology · doi:10.1007/bf00004024 · Cited by 465 · see citing works
Antioxidant activity of different fractions of Spirulina platensis protean extract
Piñero Estrada, J. (2001) · Il Farmaco · doi:10.1016/s0014-827x(01)01084-9 · Cited by 447 · see citing works
Antioxidant activity of the microalga Spirulina maxima
Miranda, M. S., Cintra, R. M. G. C., Barros, S. B. D. M., & Mancini-Filho, J. (1998) · Brazilian Journal of Medical and Biological Research · doi:10.1590/s0100-879x1998000800007 · Cited by 390 · see citing works
Novel biomarkers of the metabolism of caffeic acid derivatives in vivo
Rechner, A. R., Spencer, J. P. E., Kuhnle, G., Hahn, U., & Rice-Evans, C. A. (2001) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(01)00506-8 · Cited by 255 · see citing works
Production of spirulina biomass: Effects of environmental factors and population density
Vonshak, A., Abeliovich, A., Boussiba, S., Arad, S. M., & RICHMOND, A. E. (1982) · Biomass · doi:10.1016/0144-4565(82)90028-2 · Cited by 237 · see citing works
Olguín, E. J., Galicia, S., Angulo‐Guerrero, O., & Hernández, E. (2001) · Bioresource Technology · doi:10.1016/s0960-8524(00)00142-5 · Cited by 163 · see citing works
modelling of Spirulina Platensis growth in fresh water using response surface methodology
Vieira Costa, J. A., Colla, L. M., Filho, P. D., Kabke, K., & Weber, A. (2002) · World Journal of Microbiology and Biotechnology · doi:10.1023/a:1016822717583 · Cited by 162 · see citing works
Self-citationDuval, B., & Shetty, K. (2001) · Journal of Food Biochemistry · doi:10.1111/j.1745-4514.2001.tb00746.x · Cited by 155 · see citing works
Torzillo, G., Bernardini, P., & Masojídek, J. (1998) · Journal of Phycology · doi:10.1046/j.1529-8817.1998.340504.x · Cited by 137 · see citing works
Modelling of growth conditions for cyanobacterium Spirulina platensis in microcosms
Costa, J. A. V., Linde, G. A., Atala, D. I. P., Mibielli, G. M., & Krüger, R. T. (2000) · World Journal of Microbiology and Biotechnology · doi:10.1023/a:1008992826344 · Cited by 104 · see citing works
Self-citationQiang, H., Guterman, H., & RICHMOND, A. E. (1996) · Journal of Phycology · doi:10.1111/j.0022-3646.1996.01066.x · Cited by 90 · see citing works
Vieira Costa, J. A., Colla, L. M., & Filho, P. D. (2003) · Zeitschrift für Naturforschung C · doi:10.1515/znc-2003-1-214 · Cited by 68 · see citing works
Self-citationProduction and partial purification of γ-linolenic acid and some pigments fromSpirulina platensis
Cohen, Z., Reungjitchachawali, M., Siangdung, W., & Tanticharoen, M. (1993) · Journal of Applied Phycology · doi:10.1007/bf02182428 · Cited by 66 · see citing works
Optimization of γ-linolenic acid (GLA) production inSpirulina platensis
Tanticharoen, M., Reungjitchachawali, M., Boonag, B., Vonktaveesuk, P., Vonshak, A., & Cohen, Z. (1994) · Journal of Applied Phycology · doi:10.1007/bf02181942 · Cited by 55 · see citing works
Computer control of carbon–nitrogen ratio in Spirulina platensis
Walach, M. R., Bazin, M. J., PIRT, S. J., et al. (1987) · Biotechnology and Bioengineering · doi:10.1002/bit.260290417 · Cited by 32 · see citing works
Influence of ammonium chloride on growth and fatty acid production by spirulina platensis
Manabe, E., Hirano, M., Takano, H., Ishikawa-Doi, N., Sode, K., & Matsunaga, T. (1992) · Applied Biochemistry and Biotechnology · doi:10.1007/bf02920551 · Cited by 13 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Microalgae Metabolites: A Rich Source for Food and Medicine
Sathasivam, R., Radhakrishnan, R., Hashem, A., & Abd_Allah, E. F. (2019) · Saudi Journal of Biological Sciences · doi:10.1016/j.sjbs.2017.11.003 · Cited by 872 · see citing works
Exploring the potential of microalgae for new biotechnology applications and beyond: a review
Rizwan, M., Mujtaba, G., Memon, S. A., Lee, K., & Rashid, N. (2018) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2018.04.034 · Cited by 578 · see citing works
Markou, G., & Georgakakis, D. (2011) · Applied Energy · doi:10.1016/j.apenergy.2010.12.042 · Cited by 577 · see citing works
Microalgal Biostimulants and Biofertilisers in Crop Productions
Ronga, D., Biazzi, E., Parati, K., Carminati, D., Carminati, E., & Tava, A. (2019) · Agronomy · doi:10.3390/agronomy9040192 · Cited by 536 · see citing works
Microalgae for “Healthy” Foods-Possibilities and Challenges
Chacón‐Lee, T. L., & González‐Mariño, G. E. (2010) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/j.1541-4337.2010.00132.x · Cited by 428 · see citing works
Thermochemical Conversion of Microalgal Biomass for Biofuel Production
Raheem, A., Wan Azlina, W. A. K. G., Taufiq Yap, Y. H., Danquah, M. K., & Harun, R. (2015) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2015.04.186 · Cited by 349 · see citing works
Potential application of microalga <i>Spirulina platensis</i> as a protein source
Lupatini, A. L., Colla, L. M., Canan, C., & Colla, E. (2017) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.7987 · Cited by 291 · see citing works
Self-citationFactors Affecting Algae Biofilm Growth and Lipid Production: A Review
Schnurr, P. J., & Allen, D. G. (2015) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2015.07.090 · Cited by 276 · see citing works
Microalgae Encapsulation Systems for Food, Pharmaceutical and Cosmetics Applications
Vieira, M. V., Pastrana, L. M., & Fuciños, P. (2020) · Marine Drugs · doi:10.3390/md18120644 · Cited by 205 · see citing works
Health promoting functional lipids from microalgae pool: A review
Katiyar, R., & Arora, A. (2020) · Algal Research · doi:10.1016/j.algal.2020.101800 · Cited by 185 · see citing works
Microalgal Cultivation for Value-Added Products: A Critical Enviro-Economical Assessment
Kothari, R., Pandey, A., Ahmad, S., Kumar, A., Pathak, V. V., & Tyagi, V. V. (2017) · 3 Biotech · doi:10.1007/s13205-017-0812-8 · Cited by 116 · see citing works
Markou, G., Chatzipavlidis, I., & Georgakakis, D. (2012) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-012-1076-4 · Cited by 114 · see citing works
Sánchez-Bayo, A., Morales, V., Rodríguez, R., Vicente, G., & Bautista, L. F. (2020) · Molecules · doi:10.3390/molecules25122834 · Cited by 110 · see citing works
Extraction and Characterization of Polyhydroxyalkanoates from Marine Green Alga and Cyanobacteria
Roja, K., Ruben Sudhakar, D., Anto, S., & Mathimani, T. (2019) · Biocatalysis and Agricultural Biotechnology · doi:10.1016/j.bcab.2019.101358 · Cited by 108 · see citing works
Antioxidant activities of Phycocyanin: A bioactive compound from Spirulina platensis
Gabr, G. A., El-Sayed, S. M., & Hikal, M. S. (2020) · Journal of Pharmaceutical Research International · doi:10.9734/jpri/2020/v32i230407 · Cited by 109 · see citing works
Rodrigues, M. S., Ferreira, L. S., Converti, A., Sato, S., & Carvalho, J. C. M. (2010) · Bioresource Technology · doi:10.1016/j.biortech.2010.01.054 · Cited by 85 · see citing works
Çelekli, A., & Yavuzatmaca, M. (2009) · Bioresource Technology · doi:10.1016/j.biortech.2008.09.042 · Cited by 80 · see citing works
Challenges in Functional Food Products with the Incorporation of Some Microalgae
Çelekli, A., Özbal, B., & Bozkurt, H. (2024) · Foods · doi:10.3390/foods13050725 · Cited by 80 · see citing works
Pão Sem Glúten Enriquecido Com a Microalga Spirulina platensis
Silva FIGUEIRA, F. D., Moraes CRIZEL, T. D., Rubira SILVA, C., & las Mercedes SALAS-MELLADO, M. D. (2011) · Brazilian Journal of Food Technology · doi:10.4260/bjft2011140400037 · Cited by 71 · see citing works
Impact of Different Storage Methods on Bioactive Compounds in Arthrospira platensis Biomass
Papalia, T., Sidari, R., & Panuccio, M. R. (2019) · Molecules · doi:10.3390/molecules24152810 · Cited by 69 · see citing works
Abdel-Latif, H. M. R., El-Ashram, S., Yilmaz, S., Naiel, M. A. E., Abdul Kari, Z., Hamid, N. K. A., Dawood, M. A. O., Nowosad, J., & Kucharczyk, D. (2022) · Aquaculture Reports · doi:10.1016/j.aqrep.2022.101135 · Cited by 62 · see citing works
Deschoenmaeker, F., Bayon-Vicente, G., Sachdeva, N., Depraetere, O., Cabrera Pino, J. C., Leroy, B., Muylaert, K., & Wattiez, R. (2017) · Bioresource Technology · doi:10.1016/j.biortech.2017.03.145 · Cited by 44 · see citing works
Casazza, A. A., Ferrari, P. F., Aliakbarian, B., Converti, A., & Perego, P. (2015) · Algal Research · doi:10.1016/j.algal.2015.09.012 · Cited by 35 · see citing works
Gün, D., Çelekli, A., Bozkurt, H., & Kaya, S. (2022) · Journal of Applied Phycology · doi:10.1007/s10811-022-02702-5 · Cited by 18 · see citing works
Nikolova, K., Gentscheva, G., Gyurova, D., Pavlova, V., Dincheva, I., Velikova, M., Gerasimova, A., Makedonski, L., & Gergov, G. (2024) · Life · doi:10.3390/life14020174 · Cited by 7 · see citing works
Dubey, S., Singhania, R. R., Ramanujam, P. K., Chen, C., Dong, C., & Patel, A. K. (2025) · Physiologia Plantarum · doi:10.1111/ppl.70186 · Cited by 3 · see citing works
- Authors
- Colla, L. M., Oliveira Reinehr, C., Reichert, C., & Costa, J. A. V.
- Affiliations
- Universidade Federal do Rio Grande (BR)
- Corresponding author
- Costa, Jorge Alberto Vieira
- Venue
- Bioresource Technology
- Publisher
- Elsevier BV
- Published
- 2007-05
- Volume
- 98
- Issue
- 7
- Pages
- 1489-1493
- ISSN
- 0960-8524 (ISSN-L)
- OpenAlex
- W2097037264
- Cited by
- 331
- References
- 21
- Citations by year
- 2026: 8 · 2025: 16 · 2024: 14 · 2023: 22 · 2022: 28 · 2021: 15 · 2020: 28 · 2019: 18 · 2018: 14 · 2017: 24 · 2016: 16 · 2015: 24 · 2014: 21 · 2013: 21 · 2012: 12
- FWCI
- 6.96
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bioresource Technology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Keywords
- Spirulina platensis, cyanobacteria cultivation, temperature effects, nitrogen content, sodium nitrate, biomass production, protein content, lipid content, phenolic compounds, nutraceuticals, cultivation cost reduction
- MeSH terms
- Acids, Carbocyclic, Analysis of Variance, Bacterial Proteins, Lipids, Nitrogen, Temperature, Biomass, Bioreactors, Dietary Supplements, Spirulina
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review