Cited by 181Algal biology and biofuel productionMicrobial Metabolic Engineering and BioproductionLipid metabolism and biosynthesis

Targeted knockout of phospholipase A2 to increase lipid productivity in Chlamydomonas reinhardtii for biodiesel production

Shin, Ye Sol, Jeong, Jooyeon, Nguyen, Thu Ha Thi, Kim, Jaoon Young Hwan, Jin, EonSeon, Sim, Sang Jun·2019·Bioresource Technology

Abstract

Biofuel derived from microalgae have several advantages over other oleaginous crops, however, still needs to be improved with its cost aspect and can be achieved by developing of a strain with improved lipid productivity. In this study, the CRISPR-Cas9 system was incorporated to carry out a target-specific knockout of the phospholipase A 2 gene in Chlamydomonas reinhardtii. The targeted gene encodes a key enzyme in the Lands cycle. As a result, the mutants showed a characteristic of increased diacylglycerol pool, followed by a higher accumulation of triacylglycerol without being significantly compensated with the cell growth. As a result, the overall lipid productivities of phospholipase A 2 knockout mutants have increased by up to 64.25% (to 80.92 g L-1 d-1). This study can provide crucial information for the biodiesel industry.

Integrity signals

Registry

Crossref · 10.1016/j.biortech.2018.09.121

Cites

49 of 49 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Shin, Y. S., Jeong, J., Nguyen, T. H. T., Kim, J. Y. H., Jin, E., & Sim, S. J.
Affiliations
  • Korea University (KR)
  • Hanyang University (KR)
  • Marine Biodiversity Institute of Korea (KR)
Corresponding author
Sim, Sang Jun
Venue
Bioresource Technology
Publisher
Elsevier BV
Published
2019-01
Volume
271
Pages
368-374
ISSN
0960-8524 (ISSN-L)
OpenAlex
W2894197736
Cited by
179
References
48
Citations by year
2026: 17 · 2025: 33 · 2024: 28 · 2023: 20 · 2022: 30 · 2021: 14 · 2020: 20 · 2019: 16 · 2018: 1
FWCI
2.94
Type
journal article
Language
en
Open access
closed
Locations
  • Bioresource Technology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Research Foundation of Korea (NRF-2016R1A2A1A05005465)
  • Ministry of Trade, Industry and Energy (20172010202050)
Topics
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Keywords
phospholipase A2, gene knockout, CRISPR/Cas9, Chlamydomonas reinhardtii, lipid productivity, triglyceride accumulation, diacylglycerol, lipid metabolism, Lands cycle, microalgae biodiesel, oleaginous microalgae
MeSH terms
Lipids, Triglycerides, Chlamydomonas reinhardtii, Phospholipases A2, Biofuels

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review