Targeted knockout of phospholipase A2 to increase lipid productivity in Chlamydomonas reinhardtii for biodiesel production
Abstract
Biofuel derived from microalgae have several advantages over other oleaginous crops, however, still needs to be improved with its cost aspect and can be achieved by developing of a strain with improved lipid productivity. In this study, the CRISPR-Cas9 system was incorporated to carry out a target-specific knockout of the phospholipase A 2 gene in Chlamydomonas reinhardtii. The targeted gene encodes a key enzyme in the Lands cycle. As a result, the mutants showed a characteristic of increased diacylglycerol pool, followed by a higher accumulation of triacylglycerol without being significantly compensated with the cell growth. As a result, the overall lipid productivities of phospholipase A 2 knockout mutants have increased by up to 64.25% (to 80.92 g L-1 d-1). This study can provide crucial information for the biodiesel industry.
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Target-specific knockout of the phospholipase A2 gene in C. reinhardtii improved overall lipid production by up to 64.25%.
“Target-specific knockout of the phospholipase A2 gene in C. reinhardtii improved overall lipid production by up to 64.25% [185].”
From Microalgae biofuels: illuminating the path to a sustainable future amidst challenges and opportunities (2024) · cites this paper as [185]
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Self-citation
- Authors
- Shin, Y. S., Jeong, J., Nguyen, T. H. T., Kim, J. Y. H., Jin, E., & Sim, S. J.
- Affiliations
- Korea University (KR)
- Hanyang University (KR)
- Marine Biodiversity Institute of Korea (KR)
- Corresponding author
- Sim, Sang Jun
- Venue
- Bioresource Technology
- Publisher
- Elsevier BV
- Published
- 2019-01
- Volume
- 271
- Pages
- 368-374
- ISSN
- 0960-8524 (ISSN-L)
- OpenAlex
- W2894197736
- Cited by
- 179
- References
- 48
- Citations by year
- 2026: 17 · 2025: 33 · 2024: 28 · 2023: 20 · 2022: 30 · 2021: 14 · 2020: 20 · 2019: 16 · 2018: 1
- FWCI
- 2.94
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bioresource Technology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Research Foundation of Korea (NRF-2016R1A2A1A05005465)
- Ministry of Trade, Industry and Energy (20172010202050)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- phospholipase A2, gene knockout, CRISPR/Cas9, Chlamydomonas reinhardtii, lipid productivity, triglyceride accumulation, diacylglycerol, lipid metabolism, Lands cycle, microalgae biodiesel, oleaginous microalgae
- MeSH terms
- Lipids, Triglycerides, Chlamydomonas reinhardtii, Phospholipases A2, Biofuels
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