A paper strip based non-invasive glucose biosensor for salivary analysis
Abstract
In our present study, we developed an optical biosensor for direct determination of salivary glucose by using immobilized glucose oxidase enzyme on filter paper strip (specific activity 1.4 U/strip) and then reacting it with synthetic glucose samples in presence of co-immobilized color pH indicator. The filter paper changed color based on concentration of glucose in reaction media and hence, by scanning this color change (using RGB profiling) through an office scanner and open source image processing software (GIMP) the concentration of glucose in the reaction medium could be deduced. Once the biosensor was standardized, the synthetic glucose sample was replaced with human saliva from donors. The individual's blood glucose level at the time of obtaining saliva was also measured using an Accuchek(™) active glucometer (Roche Inc.). In this preliminary study, a correlation of nearly 0.64 was found between glucose levels in saliva and blood of healthy individuals and in diabetic patients it was nearly in the order of 0.95, thereby validating the importance of salivary analysis. The RGB profiling method obtained a detection range of 9-1350 mg/dL glucose at a response time of 45 s and LOD of 22.2 mg/dL.
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Because saliva can be readily collected, it has been used in connection to in vitro diagnostic biosensors on strips or portable device platforms.
“Because saliva can be readily collected123-125, it has been used in connection to in vitro diagnostic biosensors on strips or portable device platforms,126-134.”
From Wearable biosensors for healthcare monitoring (2019) · cites this paper as [126]
Soni et al. demonstrated the correlation between blood and saliva glucose concentrations to be R = 0.64 in healthy subjects, whereas diabetic subjects show a much closer relationship with R = 0.95.
“Soni et al.126 have demonstrated the correlation between blood and saliva glucose concentrations to be R = 0.64 in healthy subjects, whereas diabetic subjects show a much closer relationship with R = 0.95.”
From Wearable biosensors for healthcare monitoring (2019) · cites this paper as [126]
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- Authors
- Soni, A., & Jha, S. K.
- Affiliations
- Indian Institute of Technology Delhi (IN)
- Corresponding author
- Jha, Sandeep Kumar
- ORCID
- Jha, Sandeep Kumar
- Venue
- Biosensors and Bioelectronics
- Publisher
- Elsevier BV
- Published
- 2015-05
- Volume
- 67
- Pages
- 763-768
- ISSN
- 0956-5663 (ISSN-L)
- OpenAlex
- W2079524492
- Cited by
- 173
- References
- 12
- Citations by year
- 2026: 9 · 2025: 12 · 2024: 15 · 2023: 14 · 2022: 25 · 2021: 21 · 2020: 15 · 2019: 17 · 2018: 15 · 2017: 13 · 2016: 15 · 2015: 2
- FWCI
- 5.19
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biosensors and Bioelectronics (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Indian Institute of Technology Delhi
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Biosensors and Analytical Detection
- Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrochemical sensors and biosensors
- Physical Sciences › Chemical Engineering › Bioengineering › Analytical Chemistry and Sensors
- Keywords
- salivary glucose, optical biosensors, glucose oxidase immobilization, filter paper strips, image processing, diabetes mellitus, non-invasive glucose monitoring
- MeSH terms
- Blood Glucose, Diabetes Mellitus, Enzymes, Immobilized, Glucose, Glucose Oxidase, Humans, Paper, Saliva, Biosensing Techniques
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