Lesional Accumulation of CD163+ Macrophages/microglia in Rat Traumatic Brain Injury
Abstract
A robust neuroinflammation, contributing to the development of secondary injury, is a common histopathological feature of traumatic brain injury (TBI). Characterization of leukocytic subpopulations contributing to the early infiltration of the damaged tissue might aid in further understanding of lesion development. Reactive macrophages/microglia can exert protective or damaging effects in TBI. CD163 is considered a marker of M2 (alternatively activated) macrophages. Therefore we investigated the accumulation of CD163(+) macrophages/microglia in the brain of TBI rats. TBI was induced in rats using an open skull weight-drop contusion model and the accumulation of CD163(+) cells was analyzed by immunohistochemistry. In normal rat brains, CD163 was expressed by meningeal, choroid plexus and perivascular macrophages. Significant parenchymal CD163(+) cell accumulation was observed two days post TBI and continuously increased in the investigated survival time. The accumulated CD163(+) cells were mainly distributed to the lesional areas and exhibited macrophage phenotypes with amoeboid morphologic characteristics but not activated microglial phenotypes with hypertrophic morphology and thick processes. Double-labeling experiments showed that most CD163(+) cells co-expressed heme oxygenase-1 (HO-1). In addition, in vitro incubating of macrophage RAW264.7 cells or primary peritoneal macrophages with hemoglobin- haptoglobin (Hb-Hp) complex suppressed LPS-induced inflammatory macrophages…
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CD163 is expressed exclusively on perivascular macrophages in the brain under normal conditions, as well as peripheral cells of monocyte lineage.
“CD163 is a hemoglobin-haptoglobulin scavenger receptor that is considered to be expressed exclusively on perivascular macrophages in the brain under normal conditions, as well as peripheral cells of monocyte lineage [38, 56].”
From Loss of homeostatic microglial phenotype in CSF1R-related Leukoencephalopathy (2020) · cites this paper as [56]
Brain parenchymal CD163 positive macrophages correlate with blood-brain barrier damage in other white matter disorders.
“Brain parenchymal CD163 positive macrophages correlate with blood-brain barrier damage in other white matter disorders [5, 45, 56].”
From Loss of homeostatic microglial phenotype in CSF1R-related Leukoencephalopathy (2020) · cites this paper as [56]
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- Authors
- Zhang, Z., Zhang, Z.-Y., Wu, Y., & Schluesener, H. J.
- Affiliations
- Army Medical University (CN)
- University of Tübingen (DE)
- Corresponding author
- Zhang, Zhiren
- Venue
- Brain Research
- Publisher
- Elsevier BV
- Published
- 2012-06
- Volume
- 1461
- Pages
- 102-110
- ISSN
- 0006-8993 (ISSN-L)
- OpenAlex
- W2150660037
- Cited by
- 83
- References
- 50
- Citations by year
- 2026: 3 · 2025: 6 · 2024: 2 · 2023: 6 · 2022: 10 · 2021: 3 · 2020: 10 · 2019: 7 · 2018: 2 · 2017: 7 · 2016: 3 · 2015: 6 · 2014: 7 · 2013: 10 · 2012: 1
- FWCI
- 5.01
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Natural Science Foundation Project (2010BB5025)
- National Nature Science Foundation of China (81070954)
- Topics
- Health Sciences › Medicine › Neurology › Traumatic Brain Injury and Neurovascular Disturbances
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Health Sciences › Medicine › Epidemiology › Traumatic Brain Injury Research
- Keywords
- traumatic brain injury, CD163, M2 macrophages, microglia, neuroinflammation, heme oxygenase-1, hemoglobin-haptoglobin complex, anti-inflammatory macrophages, secondary injury, contusion injury model, RAW 264.7 macrophages, peritoneal macrophages
- MeSH terms
- CD163 Antigen, Animals, Brain Injuries, Cell Line, Macrophages, Male, Rats, Inbred Lew, Receptors, Cell Surface, Antigens, Differentiation, Myelomonocytic, Antigens, CD, Microglia, Mice, Rats
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