Cited by 211Pluripotent Stem Cells ResearchNeurogenesis and neuroplasticity mechanismsNeuroinflammation and Neurodegeneration Mechanisms

Human Stem Cell-Derived Spinal Cord Astrocytes with Defined Mature or Reactive Phenotypes

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Roybon, Laurent, Lamas, Nuno J., Garcia-Diaz, Alejandro, Yang, Eun Ju, Sattler, Rita, Jackson-Lewis, Vernice, Kim, Yoon A., Kachel, C. Alan +4 more·2013·Cell Reports

Abstract

Differentiation of astrocytes from human stem cells has significant potential for analysis of their role in normal brain function and disease, but existing protocols generate only immature astrocytes. Using early neuralization, we generated spinal cord astrocytes from mouse or human embryonic or induced pluripotent stem cells with high efficiency. Remarkably, short exposure to fibroblast growth factor 1 (FGF1) or FGF2 was sufficient to direct these astrocytes selectively toward a mature quiescent phenotype, as judged by both marker expression and functional analysis. In contrast, tumor necrosis factor alpha and interleukin-1β, but not FGFs, induced multiple elements of a reactive inflammatory phenotype but did not affect maturation. These phenotypically defined, scalable populations of spinal cord astrocytes will be important both for studying normal astrocyte function and for modeling human pathological processes in vitro.

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Crossref · 10.1016/j.celrep.2013.06.021

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  1. Reilly JF Maher PA Kumari VG Regulation of astrocyte GFAP expression by TGF-beta1 and FGF-2 Glia 1998 22 202 210 9537840

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Authors
Roybon, L., Lamas, N. J., Garcia-Diaz, A., Yang, E. J., Sattler, R., Jackson-Lewis, V., Kim, Y. A., Kachel, C. A., Rothstein, J. D., Przedborski, S., Wichterle, H., & Henderson, C. E.
Affiliations
  • Columbia University Irving Medical Center (US)
  • Johns Hopkins University (US)
Corresponding author
Henderson, Christopher E.
Venue
Cell Reports
Publisher
Elsevier BV
Published
2013-09
Volume
4
Issue
5
Pages
1035-1048
ISSN
2211-1247 (ISSN-L)
OpenAlex
W2009867508
Cited by
208
References
104
Citations by year
2026: 1 · 2025: 14 · 2024: 19 · 2023: 12 · 2022: 33 · 2021: 22 · 2020: 17 · 2019: 14 · 2018: 19 · 2017: 18 · 2016: 12 · 2015: 12 · 2014: 12 · 2013: 1
FWCI
5.53
Type
journal article
Language
en
Open access
Locations
  • Cell Reports (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
National Institute of Neurological Disorders and Stroke (R01NS042269)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
Keywords
spinal astrocytes, human stem cells, astrocyte maturation, reactive astrocytes, induced pluripotent stem cells, embryonic stem cells, fibroblast growth factor 1, fibroblast growth factor 2, TNF-α, IL-1β, astrocyte differentiation, in vitro disease model
MeSH terms
Animals, Astrocytes, Cell Differentiation, Cells, Cultured, Humans, Neurons, Phenotype, Spinal Cord, Pluripotent Stem Cells, Mice, Neural Stem Cells

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