Human Stem Cell-Derived Spinal Cord Astrocytes with Defined Mature or Reactive Phenotypes
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Differentiation of astrocytes from human stem cells has significant potential for analysis of their role in normal brain function and disease, but existing protocols generate only immature astrocytes. Using early neuralization, we generated spinal cord astrocytes from mouse or human embryonic or induced pluripotent stem cells with high efficiency. Remarkably, short exposure to fibroblast growth factor 1 (FGF1) or FGF2 was sufficient to direct these astrocytes selectively toward a mature quiescent phenotype, as judged by both marker expression and functional analysis. In contrast, tumor necrosis factor alpha and interleukin-1β, but not FGFs, induced multiple elements of a reactive inflammatory phenotype but did not affect maturation. These phenotypically defined, scalable populations of spinal cord astrocytes will be important both for studying normal astrocyte function and for modeling human pathological processes in vitro.
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GFAP is expressed by progenitor cells and its expression depends on developmental stages.
“GFAP is also expressed by progenitor cells15 and its expression depends on developmental stages16,17.”
From Reactive astrocyte nomenclature, definitions, and future directions (2021) · cites this paper as [17]
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Hagman, S., Mäkinen, A., Ylä-Outinen, L., Huhtala, H., Elovaara, I., & Narkilahti, S. (2019) · Journal of Neuroimmunology · doi:10.1016/j.jneuroim.2018.07.010 · Cited by 31 · see citing works
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Assessing Gq-GPCR–induced human astrocyte reactivity using bioengineered neural organoids
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Modeling Cell-Cell Interactions in Parkinson’s Disease Using Human Stem Cell-Based Models
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New In Vitro Models to Study Amyotrophic Lateral Sclerosis
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The Importance of Non-neuronal Cell Types in hiPSC-Based Disease Modeling and Drug Screening
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Dystrophin deficiency leads to dysfunctional glutamate clearance in iPSC derived astrocytes
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Tissue-Engineered Models of the Human Brain: State-of-the-Art Analysis and Challenges
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Self-citationModeling Neurological Disorders with Human Pluripotent Stem Cell-Derived Astrocytes
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Stem Cell Neurodevelopmental Solutions for Restorative Treatments of the Human Trunk and Spine
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Mapping Astrocyte Transcriptional Signatures in Response to Neuroactive Compounds
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- Authors
- Roybon, L., Lamas, N. J., Garcia-Diaz, A., Yang, E. J., Sattler, R., Jackson-Lewis, V., Kim, Y. A., Kachel, C. A., Rothstein, J. D., Przedborski, S., Wichterle, H., & Henderson, C. E.
- Affiliations
- Columbia University Irving Medical Center (US)
- Johns Hopkins University (US)
- Corresponding author
- Henderson, Christopher E.
- ORCID
- Venue
- Cell Reports
- Publisher
- Elsevier BV
- Published
- 2013-09
- Volume
- 4
- Issue
- 5
- Pages
- 1035-1048
- ISSN
- 2211-1247 (ISSN-L)
- PMC
- PMC4229657
- OpenAlex
- W2009867508
- Cited by
- 208
- References
- 104
- Citations by year
- 2026: 1 · 2025: 14 · 2024: 19 · 2023: 12 · 2022: 33 · 2021: 22 · 2020: 17 · 2019: 14 · 2018: 19 · 2017: 18 · 2016: 12 · 2015: 12 · 2014: 12 · 2013: 1
- FWCI
- 5.53
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Cell Reports (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Institute of Neurological Disorders and Stroke (R01NS042269)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- spinal astrocytes, human stem cells, astrocyte maturation, reactive astrocytes, induced pluripotent stem cells, embryonic stem cells, fibroblast growth factor 1, fibroblast growth factor 2, TNF-α, IL-1β, astrocyte differentiation, in vitro disease model
- MeSH terms
- Animals, Astrocytes, Cell Differentiation, Cells, Cultured, Humans, Neurons, Phenotype, Spinal Cord, Pluripotent Stem Cells, Mice, Neural Stem Cells
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