In Vivo Interrogation of Spinal Mechanosensory Circuits
Open accessAbstract
Spinal dorsal horn circuits receive, process, and transmit somatosensory information. To understand how specific components of these circuits contribute to behavior, it is critical to be able to directly modulate their activity in unanesthetized in vivo conditions. Here, we develop experimental tools that enable optogenetic control of spinal circuitry in freely moving mice using commonly available materials. We use these tools to examine mechanosensory processing in the spinal cord and observe that optogenetic activation of somatostatin-positive interneurons facilitates both mechanosensory and itch-related behavior, while reversible chemogenetic inhibition of these neurons suppresses mechanosensation. These results extend recent findings regarding the processing of mechanosensory information in the spinal cord and indicate the potential for activity-induced release of the somatostatin neuropeptide to affect processing of itch. The spinal implant approach we describe here is likely to enable a wide range of studies to elucidate spinal circuits underlying pain, touch, itch, and movement.
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The Reln dorsal excitatory family comprised Excit-8, Excit-9, Excit-10, and Excit-11 and expressed markers indicative of a role in chemical itch sensation.
“The Reln dorsal, excitatory family was comprised of Excit-8, Excit-9, Excit-10, and Excit-11 and expressed markers indicative of a role in chemical itch sensation58–62.”
From A harmonized atlas of mouse spinal cord cell types and their spatial organization (2021) · cites this paper as [59]
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Central Nervous System Pain Pathways
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Next generation behavioral sequencing for advancing pain quantification
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Inflammatory pain neural plasticity
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Probing the coding logic of thermosensation using spinal cord calcium imaging
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Counter-stimuli Inhibit GRPR Neurons via GABAergic Signaling in the Spinal CordPreprint
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- Authors
- Christensen, A. J., Iyer, S. M., François, A., Vyas, S., Ramakrishnan, C., Vesuna, S., Deisseroth, K., Scherrer, G., & Delp, S. L.
- Affiliations
- Stanford University (US)
- Stanford Medicine (US)
- Howard Hughes Medical Institute (US)
- Corresponding author
- Scherrer, Grégory
- Delp, Scott L.
- ORCID
- Venue
- Cell Reports
- Publisher
- Elsevier BV
- Published
- 2016-11
- Volume
- 17
- Issue
- 6
- Pages
- 1699-1710
- ISSN
- 2211-1247 (ISSN-L)
- PMC
- PMC5507199
- OpenAlex
- W2547032004
- Cited by
- 75
- References
- 46
- Citations by year
- 2026: 2 · 2025: 6 · 2024: 4 · 2023: 4 · 2022: 11 · 2021: 4 · 2020: 12 · 2019: 15 · 2018: 11 · 2017: 6
- FWCI
- 8.59
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Cell Reports (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- HAL (submittedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- NIH (R01-NS080954, DA031777)
- Stanford Bio-X NeuroVentures
- Texas Instruments Stanford Graduate Fellowship
- Howard Hughes Medical Institute International Student Research Fellowship
- Siebel Scholars Foundation
- Rita Allen Foundation
- American Pain Society
- Topics
- Health Sciences › Medicine › Physiology › Pain Mechanisms and Treatments
- Life Sciences › Neuroscience › Sensory Systems › Ion Channels and Receptors
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- dorsal horn, mechanosensing, somatostatin interneurons, optogenetic stimulation, chemogenetic inhibition, pruritus, spinal implants, freely moving mice, somatosensory processing, spinal cord circuits
- MeSH terms
- Animals, Female, Histamine, Interneurons, Light, Mice, Inbred C57BL, Pruritus, Somatostatin, Spinal Cord, Proto-Oncogene Proteins c-fos, Mechanotransduction, Cellular, Optical Fibers, Optogenetics
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