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Polyphenol contents and antioxidant activity of lyophilized aqueous extract of propolis from Erzurum, Turkey

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Gülçin, İlhami, Bursal, Ercan, Şehitoğlu, M. Hilal, Bilsel, Mine, Gören, Ahmet C.·2010·Food and Chemical Toxicology
DOI 10.1016/j.fct.2010.05.053
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Abstract

Propolis, an extremely complex resinous material, exhibits valuable pharmacological and biological properties attributed to the presence of polyphenols. In this study, we determined the antioxidant properties of lyophilized aqueous extract of propolis (LAEP) from Erzurum province of Turkey and correlated the values with total levels of polyphenolic compounds. In order to estimate the capacity of LAEP to act as antioxidants, we studied its 1,1-diphenyl-2-picryl-hydrazyl radicals (DPPH()), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radicals (ABTS()(+)), N,N-dimethyl-p-phenylenediamine radicals (DMPD()(+)), superoxide anion radicals (O(2)(-)) and hydrogen peroxide (H(2)O(2)) scavenging activity, total antioxidant activity, ferric ions (Fe(3+)) and cupric ions (Cu(2+)) reducing ability, ferrous ions (Fe(2+)) chelating activity. LAEP inhibited 93.2% lipid peroxidation of a linoleic acid emulsion at 30 microg/mL concentration. On the other hand, BHA, BHT, alpha-tocopherol and trolox displayed 83.3%, 82.1%, 68.1% and 81.3% inhibition of peroxidation at the same concentration, respectively. Quantitative amounts of caffeic acid, ferulic acid, syringic acid, ellagic acid, quercetin, alpha-tocopherol, pyrogallol, p-hydroxybenzoic acid, vanillin, p-coumaric acid, gallic acid and ascorbic acid were detected by high performance liquid chromatography and tandem mass spectrometry (LC-MS/MS). This study will bring an innovation for further studies with regard to the antioxidant properties of LAEP.

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Metadata

Authors
Gülçin, İ., Bursal, E., Şehitoğlu, M. H., Bilsel, M., & Gören, A. C.
Venue
Food and Chemical Toxicology
Publisher
Elsevier BV
Published
2010-08
Volume
48
Issue
8-9
Pages
2227-2238
ISSN
0278-6915 (ISSN-L)
DOI
10.1016/j.fct.2010.05.053
OpenAlex
W2001726712
Cited by
440
References
6
FWCI
33.41
Type
journal article
Language
en
Open access
green
Licence
  • www.elsevier.com/tdm/userlicense/1.0/ (tdm)
  • www.elsevier.com/legal/tdmrep-license (tdm)

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Gülçin, İ., Bursal, E., Şehitoğlu, M. H., Bilsel, M., & Gören, A. C. (2010). Polyphenol contents and antioxidant activity of lyophilized aqueous extract of propolis from Erzurum, Turkey. Food and Chemical Toxicology. https://doi.org/10.1016/j.fct.2010.05.053

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