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Cited by 65Advancements in Transdermal Drug DeliveryAdvanced Drug Delivery SystemsWound Healing and Treatments

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Chitosan-based microneedle arrays for dermal delivery of Centella asiatica

Ryall, Cameron, Chen, Shuo, Duarah, Sanjukta, Wen, Jingyuan·2022·International Journal of Pharmaceutics
DOI 10.1016/j.ijpharm.2022.122221
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Abstract

Centella asiatica, a medicinal herb used for wound healing, has a limited effect when delivered as an ointment. Centella asiatica's active component asiatic acid (AA) increases extracellular matrix development and reduces inflammation but cannot penetrate the stratum corneum to access deeper skin layers. To bypass the stratum corneum, we formulated two types of AA-loaded microneedle arrays. We fabricated, characterised and optimised a dissolving array made from chitosan and PVA and a hydrogel array made from chitosan and PVP. Both needles were strong and long enough to pierce the epidermis without breaking. Both were biocompatible with keratinocytes and fibroblasts (>75% viability at 100% concentration) and showed a sustained drug release over 48 h. The hydrogel microneedle released more AA (52.2%) than the dissolving formulation (26.4%); thus, we evaluated them in an excisional rat model. The hydrogel microneedle arrays significantly increased the rate of wound closure compared to the control. This research has shown that the chitosan-PVA hydrogel microneedles could penetrate the epidermis, effectively release AA, and increase the wound closure rate. This AA-loaded delivery system shows promise as a natural treatment for wound healing and may be applied to other bioactive compounds with similar physiochemical properties in the future.

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Metadata

Authors
Ryall, C., Chen, S., Duarah, S., & Wen, J.
Affiliations
University of Auckland (NZ)
Corresponding author
  • Chen, Shuo
  • Wen, Jingyuan
ORCID
Venue
International Journal of Pharmaceutics
Publisher
Elsevier BV
Published
2022-11
Volume
627
Pages
122221
ISSN
0378-5173 (ISSN-L)
DOI
10.1016/j.ijpharm.2022.122221
OpenAlex
W4297091786
Cited by
65
References
2
Citations by year
2026: 18 · 2025: 13 · 2024: 24 · 2023: 10
FWCI
4.87
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • International Journal of Pharmaceutics (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • University of Auckland
  • University of Auckland Faculty of Medical and Health Sciences
Topics
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advancements in Transdermal Drug Delivery
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
  • Health Sciences › Medicine › Surgery › Wound Healing and Treatments
Keywords
Centella asiatica, asiatic acid, dermal drug delivery, wound healing, stratum corneum penetration, dissolving microneedles, sustained drug release, biocompatibility, wound closure
MeSH terms
Administration, Cutaneous, Animals, Microinjections, Needles, Ointments, Plant Extracts, Skin, Triterpenes, Drug Delivery Systems, Hydrogels, Centella, Chitosan, Rats

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