Cited by 39Sirtuins and Resveratrol in MedicineMuscle Physiology and DisordersMetabolism, Diabetes, and Cancer

Resveratrol improves muscle regeneration in obese mice through enhancing mitochondrial biogenesis

Niu, Wenjing, Wang, Haibo, Wang, Bo, Mao, Xueying, Du, Min·2021·The Journal of Nutritional Biochemistry

Abstract

Obesity is increasing rapidly worldwide and is accompanied by many complications, including impaired muscle regeneration. Obesity is known to inhibit AMP-activated protein kinase (AMPK) activity, which impedes mitochondrial biogenesis, myogenic differentiation and muscle regeneration. Resveratrol has an effective anti-obesity effect, but its effect on regeneration of muscle in obese mice remains to be tested. We hypothesized that resveratrol activates AMPK and mitochondrial biogenesis to improve muscle regeneration. Male C57BL/6J mice were fed a control diet or a 60% high-fat diet with or without resveratrol supplementation for 8 weeks and, then, the tibialis anterior muscle was subjected to cardiotoxin-induced muscle injury. Muscle tissue was collected at 3 and 7 d after injury. We found that resveratrol enhanced both proliferation and differentiation of satellite cells following injury in obese mice. Markers of mitochondrial biogenesis were upregulated in resveratrol-treated mice. In C2C12 myogenic cells, resveratrol activated AMPK and stimulated the expression of peroxisome proliferator-activated receptor gamma coactivator 1-alpha, which were associated with enhanced myogenic differentiation. Such effects of resveratrol were abolished by AMPKα1 ablation, showing the mediatory roles of AMPK. In summary, dietary resveratrol activates AMPK/ proliferator-activated receptor gamma coactivator 1-alpha axis to facilitate mitochondrial biogenesis and muscle regeneration impaired due to obesity.

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Crossref · 10.1016/j.jnutbio.2021.108804

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Authors
Niu, W., Wang, H., Wang, B., Mao, X., & Du, M.
Affiliations
  • China Agricultural University (CN)
  • Southwest Minzu University (CN)
  • State Key Laboratory of Animal Nutrition (CN)
  • Washington State University (US)
Corresponding author
Du, Min
Venue
The Journal of Nutritional Biochemistry
Publisher
Elsevier BV
Published
2021-12
Volume
98
Pages
108804
ISSN
0955-2863 (ISSN-L)
OpenAlex
W3175833626
Cited by
39
References
54
Citations by year
2026: 5 · 2025: 8 · 2024: 9 · 2023: 9 · 2022: 8
FWCI
3.60
Type
journal article
Language
en
Open access
closed
Locations
  • The Journal of Nutritional Biochemistry (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Geriatrics and Gerontology › Sirtuins and Resveratrol in Medicine
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
Keywords
resveratrol, muscle regeneration, obesity, mitochondrial biogenesis, AMPK, PGC-1α, satellite cells, myogenesis, cardiotoxin-induced muscle injury, high-fat diet, AMPKα1, tibialis anterior
MeSH terms
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha, Resveratrol, Animals, Antioxidants, Organelle Biogenesis, Cell Differentiation, Male, Mice, Inbred C57BL, Mice, Obese, Mitochondria, Muscle, Obesity, Regeneration, Muscle, Skeletal, Muscle Development, Mice, AMP-Activated Protein Kinases, Diet, High-Fat

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