Centella asiatica extract protects against amyloid β1–40-induced neurotoxicity in neuronal cells by activating the antioxidative defence system
Open accessAbstract
Centella asiatica (雷公根 léi gōng gēn) is a traditional medicinal herb with high antioxidant activity, which decreases amyloid-β (Aβ) deposition in the brain. At the same time, aggregated Aβ-induced oxidative stress is the trigger in the pathogenesis of Alzheimer's disease (AD). Here, we investigated the ability of C. asiatica ethanol extract (CAE) to protect PC12 and IMR32 cells from Aβ1–40-induced production of reactive oxygen species (ROS) and concomitant neurotoxicity. Aggregated Aβ1–40 treatment resulted in reduced cell viability, which can be reversed by cotreatment with 25, 50, and 100 μg/mL CAE. Moreover, CAE eliminated the Aβ1–40-mediated increase in ROS production. Thus, CAE-mediated protection against aggregated Aβ1–40-induced neurotoxicity is attributable to modulation of the antioxidative defense system in cells, including the activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, and levels of glutathione and glutathione disulfide by CAE. This emphasizes the potential therapeutic and preventive value of CAE in the treatment of AD.
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Administration of C. asiatica extracts into PC12 pheochromocytoma cells and human IMR32 neuroblastoma cells bearing Aβ-induced oxidative stress results in overall antioxidant activity via modulation of the antioxidant defence system and direct elimination of uncontrolled ROS production.
“Aggregated Aβ‐induced ROS generation and subsequent neurotoxicity are critical to the pathogenesis of AD; administration of C. asiatica extracts into PC12 pheochromocytoma cells and human IMR32 neuroblastoma cells bearing Aβ‐induced oxidative stress have resulted in overall antioxidant activity. The mechanism of this action is via modulation of the antioxidant defence system and direct elimination”
From Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review (2023) · cites this paper as [59]
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Experimental — accuracy not yet measuredCentella asiatica extract inhibits Aβ-mediated reactive oxygen species production in neural cells.
“In this sequence, Chen and coworkers investigated the anti-Alzheimer's activity of CA extract and revealed the ability of CA extract to inhibit Aβ-mediated reactive oxygen species production in neural cells [110].”
From Unlocking the Therapeutic Potential of Medicinal Plants for Alzheimer’s Disease: Preclinical to Clinical Trial Insights (2023) · cites this paper as [110]
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- Authors
- Chen, C.-L., Tsai, W.-H., Chen, C.-J., & Pan, T.-M.
- Affiliations
- National Taiwan University (TW)
- Corresponding author
- Pan, Tzu-Ming
- Venue
- Journal of Traditional and Complementary Medicine
- Publisher
- Elsevier BV
- Published
- 2016-10
- Volume
- 6
- Issue
- 4
- Pages
- 362-369
- ISSN
- 2225-4110 (ISSN-L)
- PMC
- PMC5067859
- OpenAlex
- W958517079
- Cited by
- 69
- References
- 30
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 13 · 2023: 9 · 2022: 5 · 2021: 8 · 2020: 8 · 2019: 4 · 2018: 4 · 2017: 7 · 2016: 2
- FWCI
- 8.50
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Licence
- Locations
- Journal of Traditional and Complementary Medicine (publishedVersion, cc-by-nc-nd)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Science Council (NSC 98-2622-B-002-005-CC2)
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Health Sciences › Medicine › Complementary and alternative medicine › Ginkgo biloba and Cashew Applications
- Keywords
- Centella asiatica, amyloid-β1-40, Alzheimer's disease, neurotoxicity, oxidative stress, reactive oxygen species, antioxidant defense, superoxide dismutase, catalase, glutathione peroxidase, glutathione, glutathione disulfide, glutathione reductase, PC12 cells, IMR-32 cells, ethanol extract, neuroprotection
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