Centella asiatica Attenuates Mitochondrial Dysfunction and Oxidative Stress in A β‐Exposed Hippocampal Neurons
Open accessAbstract
Centella asiatica has been used for centuries to enhance memory. We have previously shown that a water extract of Centella asiatica (CAW) protects against the deleterious effects of amyloid‐β (Aβ) in neuroblastoma cells and attenuates Aβ‐induced cognitive deficits in mice. Yet, the neuroprotective mechanism of CAW has yet to be thoroughly explored in neurons from these animals. This study investigates the effects of CAW on neuronal metabolism and oxidative stress in isolated Aβ‐expressing neurons. Hippocampal neurons from amyloid precursor protein overexpressing Tg2576 mice and wild‐type (WT) littermates were treated with CAW. In both genotypes, CAW increased the expression of antioxidant response genes which attenuated the Aβ‐induced elevations in reactive oxygen species (ROS) and lipid peroxidation in Tg2576 neurons. CAW also improved mitochondrial function in both genotypes and increased the expression of electron transport chain enzymes and mitochondrial labeling, suggesting an increase in mitochondrial content. These data show that CAW protects against mitochondrial dysfunction and oxidative stress in Aβ‐exposed hippocampal neurons which could contribute to the beneficial effects of the extract observed in vivo. Since CAW also improved mitochondrial function in the absence of Aβ, these results suggest a broader utility for other conditions where neuronal mitochondrial dysfunction occurs.
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CAW increases NRF2-regulated gene transcripts and reduces Aβ-associated ROS in vitro.
“CAW increases NRF2-regulated gene transcripts, reduces Aβ-associated ROS [27]”
From Centella Asiatica Improves Memory and Promotes Antioxidative Signaling in 5XFAD Mice (2019) · cites this paper as [27]
Previous studies show CAW treatment is associated with elevated levels of NRF2-targeted ARE gene transcripts in Tg2576 primary neurons and hippocampus and cortex of young and aged WT mice.
“Previous studies in our laboratory show that CAW treatment is associated with elevated levels of NRF2-targeted ARE gene transcripts in Tg2576 primary neurons [27], as well as the hippocampus and cortex of young and aged WT mice [46].”
From Centella Asiatica Improves Memory and Promotes Antioxidative Signaling in 5XFAD Mice (2019) · cites this paper as [27]
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- Authors
- Gray, N. E., Zweig, J. A., Matthews, D. G., Caruso, M., Quinn, J. F., & Soumyanath, A.
- Affiliations
- Oregon Health & Science University (US)
- Corresponding author
- Gray, Nora E.
- Venue
- Oxidative Medicine and Cellular Longevity
- Publisher
- Wiley
- Published
- 2017-01
- Volume
- 2017
- Issue
- 1
- Pages
- 7023091-7023091
- ISSN
- 1942-0994 (ISSN-L)
- 1942-0900
- PMC
- PMC5572603
- OpenAlex
- W2744011535
- Cited by
- 60
- References
- 46
- Citations by year
- 2026: 6 · 2025: 8 · 2024: 10 · 2023: 5 · 2022: 7 · 2021: 8 · 2020: 5 · 2019: 6 · 2018: 4 · 2017: 1
- FWCI
- 6.50
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Oxidative Medicine and Cellular Longevity (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Center for Complementary and Integrative Health (K99AT008831, R01AT008099)
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Keywords
- Centella asiatica, amyloid-beta, hippocampal neurons, mitochondrial dysfunction, oxidative stress, reactive oxygen species, lipid peroxidation, electron transport chain, Tg2576 mice, neuroprotection, memory enhancement
- MeSH terms
- Animals, Hippocampus, Humans, Mitochondria, Neurons, Plant Extracts, Triterpenes, Lipid Peroxidation, Amyloid beta-Peptides, Reactive Oxygen Species, Oxidative Stress, Centella, Mice
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